Calbindin D-28K Antibody (JF05-01)

Western Blot: Calbindin D-28K Antibody (JF05-01) [NBP2-67200] - Analysis of Calbindin on different lysates using anti-Calbindin antibody at 1/1,000 dilution. Positive control: Lane 1: Rat brain Lane 2: Mouse kidney

Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (JF05-01) [NBP2-67200] - Staining Calbindin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (JF05-01) [NBP2-67200] - Analysis of paraffin-embedded rat brain tissue using anti-Calbindin antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (JF05-01) [NBP2-67200] - Staining Calbindin in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (JF05-01) [NBP2-67200] - Analysis of paraffin-embedded human kidney tissue using anti-Calbindin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (JF05-01) [NBP2-67200] - Analysis of paraffin-embedded mouse brain tissue using anti-Calbindin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (JF05-01) [NBP2-67200] - Analysis of paraffin-embedded mouse kidney tissue using anti-Calbindin antibody. Counter stained with hematoxylin.

Product Discontinued

Calbindin D-28K Antibody (JF05-01) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Calbindin D-28K aa 51-100 / 261. (SwissProt: P05937 Human; SwissProt: P12658 Mouse; SwissProt: P07171 Rat)
• Localization: Cytosol, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CALB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Calbindin D-28K Antibody (JF05-01)

• CAB27
• CALB
• calbindin 1, (28kD)
• calbindin 1, 28kDa
• Calbindin D28
• calbindin
• D-28K
• RTVL-H protein
• Vitamin D-dependent calcium-binding protein, avian-type

Background

Calbindin D28k is a member of a large family of intracellular calcium-binding proteins, containing EF-hand calcium binding motifs, and related to calmodulin and troponin-C. The biological roles of Calbindin D28k include calcium regulation and calcium-dependent signalling in neurons and during development. Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. Calbindin antibody is often used as a marker for cerebellum Purkinje cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Calbindin D-28K Antibody (NBP2-67200). (Showing 1 - 1 of 1 FAQ).

1. I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?
   • This link will take you to all of our alpha7 nicotinic cholingergic receptor antibodies currently available for purchase. Please see the following link for our Calbinin D28K antibodies reactive against mouse and human. We currently do not have any Parvalbumin antibodies that have been tested in mouse. I would like to introduce you to our Innovators Reward Program. Try our primary antibodies in an untested species or application, without the financial risk of failure. The pricing and availability of our products depends on your country.

Bioinformatics

• Gene Symbol: CALB1