Calponin 1 Antibody (CNN1/4227R)

Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/4227R) [NBP3-07589] - Formalin-fixed, paraffin-embedded human leiomyosarcoma stained with Calponin 1 Rabbit Recombinant Monoclonal Antibody (CNN1/4227R).

Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/4227R) [NBP3-07589] - Formalin-fixed, paraffin-embedded human leiomyosarcoma stained with Calponin 1 Rabbit Recombinant Monoclonal Antibody (CNN1/4227R).

• Reactivity: Hu
• Applications: IHC
• Clone: CNN1/4227R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Calponin 1 Antibody (CNN1/4227R) Summary

• Description: 200ug/ml of antibody purified by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08462)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide corresponding to residues on the C-terminus of human Calponin 1 was used as an immunogen. (Uniprot: P51911)
• Localization: Cytoplasmic
• Marker: Smooth Muscle Marker
• Specificity: Calponin a 33 kDa polypeptide, is a cytoskeleton-associated actin-binding protein that also interacts tropomyosin and calmodulin. Calponin antibody has been found to be useful as a marker for myoepithelial and basal lamina in differentiating microinvasive from in situ ductal carcinomas of the breast. Calponin antibody may also have applications in malignant myoepithelium and pleomorphic adenoma of salivary gland as well as a useful marker for fine needle aspirates of papillary breast lesions. The antibody has been found in tissue extracts from smooth muscle, but not in fibroblast extracts.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CNN1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Calponin 1 Antibody (CNN1/4227R)

• Basic calponin
• Calponin 1
• calponin 1, basic, smooth muscle
• Calponin H1
• Calponin H1, smooth muscle
• calponins, basic
• CNN1
• Sm-Calp
• SMCC
• SMCCcalponin-1

Background

Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction (1). Calponin binds filamentous actin (F-actin) through two distinct sites ABS1 and ABS2, with an affinity in the low micromolar range (2) Immunoreactivity for calponin, along with alpha-smooth muscle actin and smooth muscle myosin heavy chains, confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adenocarcinomas (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CNN1
• Uniprot:
  • P51911(Human)