cAMP Antibody (250532) [Unconjugated]

cAMP was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-cAMP Monoclonal Antibody (Catalog # MAB2146) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: All-Multi
• Applications: ICC/IF
• Clone: 250532
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

cAMP Antibody (250532) [Unconjugated] Summary

• Immunogen: Thryoglobulin-coupled cAMP
• Specificity: Detects cAMP. In a competition binding assay, this antibody does not cross-react with ATP, CTP, GTP, AMP, GMP, cGMP, or cUMP.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for cAMP Antibody (250532) [Unconjugated]

• cAMP
• Cyclic AMP

Background

cAMP is a cyclic nucleotide generated from ATP by the activity of adenylate cyclase. It is a ubiquitous cytoplasmic second messenger important in many signal transduction pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for cAMP Antibody (MAB2146)(3)

Publications using MAB2146

• Hai-Ying Shen, Sadie B. Baer, Raey Gesese, John M. Cook, Landen Weltha, Shayla Q. Coffman et al. Adenosine-A2A Receptor Signaling Plays a Crucial Role in Sudden Unexpected Death in Epilepsy Frontiers in Pharmacology 2022-06-09 [PMID: 35754505] (Immunohistochemistry, Transgenic Mouse)
• Gao Feng microRNA-208a in an early stage myocardial infarction rat model and the effect on cAMP-PKA signaling pathway Mol Med Rep, 2016-06-14;0(0):. 2016-06-14 [PMID: 27314868] (Western Blot, Human)
• Fu W, Ruangkittisakul A, MacTavish D, Shi JY, Ballanyi K, Jhamandas JH Amyloid beta (Abeta) peptide directly activates amylin-3 receptor subtype by triggering multiple intracellular signaling pathways. J. Biol. Chem., 2012-04-12;287(22):18820-30. 2012-04-12 [PMID: 22500019] (ICC, Western Blot, Human)