![ELISA: cAMP Antibody (RM466)[NBP3-18822]Competitive ELISA of cAMP using anti-cAMP (RM466). The 96-well plate was coated with 1 ug/mL of Goat anti-rabbit IgG (50 µl/well). 0.05 µg/ml of anti-cAMP(RM466) (50 µl/well) was added and incubated. After wash and block, different concentrations of cAMP (25 µl/well) were added along with 25 µl/well of 1/5,000 diluted HRP conjugated cAMP. (71-1358-00) TMB was used to develop the color after incubation and wash.](http://images.novusbio.com/fullsize/nbp3-18822_rabbit-camp-mab-rm466-16112023130187.png "ELISA: cAMP Antibody (RM466)[NBP3-18822]Competitive ELISA of cAMP using anti-cAMP (RM466). The 96-well plate was coated with 1 ug/mL of Goat anti-rabbit IgG (50 µl/well). 0.05 µg/ml of anti-cAMP(RM466) (50 µl/well) was added and incubated. After wash and block, different concentrations of cAMP (25 µl/well) were added along with 25 µl/well of 1/5,000 diluted HRP conjugated cAMP. (71-1358-00) TMB was used to develop the color after incubation and wash.")
| Reactivity | All-NASpecies Glossary |
| Applications | ELISA |
| Clone | RM466 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | KLH-conjugated cAMP |
| Specificity | This antibody reacts to cAMP. No cross reactivity with other cyclic nucleotide or nucleoside phosphate. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. |
| Buffer | 50% Glycerol/PBS, 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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There is nothing beta than PKC Alpha cAMP-dependent protein kinase (PKA) is a Ser/Thr protein kinase that is highly conserved between species. Three distinct catalytic (C) subunits have been identified, designated C-alpha, C-beta and C-gamma, where C-alpha and C-beta are most closely rel... Read full blog post. |
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CXCR4 Studies on Neural and Stem Cells The CXCR4 (C-X-C chemokine receptor type 4) protein is one member of the G-protein coupled receptor (GPCR1) family. As a multipass membrane protein that is found in several tissues, it is the receptor for the C-X-C chemokine CXCL12/SDF-1. The CXCR4 li... Read full blog post. |
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AKAP95/AKAP8 Orchestrates and Synchronizes Cellular Events A-kinase anchor proteins (AKAPs), such as AKAP95/AKAP8, are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA). AKAPs orchestrate and synchronize cellular events by tethering the cAMP-dependent PKA and oth... Read full blog post. |
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Real-time Cell Analysis Throws New Light on GPCR Function The G-protein coupled receptor (GPCR) family constitutes one of the largest protein families in the mammalian kingdom, with over 800 proteins so far discovered. Our GPCR antibody catalog covers all 6 classes, with antibodies raised against Metabotropi... Read full blog post. |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")