Caspase-12 Assay Kit (Fluorometric)

Product Discontinued

Caspase-12 Assay Kit (Fluorometric) Summary

• Description: Simple and convenient fluorometric assay to measure Caspase-12 activity within 1-2 hrs.
  
  Detection method: Fluorescence microscopy, Flow cytometry and fluorescence plate reader (Ex/Em = 400/505 nm)Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65664)
• Kit Type: Assay Kit (Fluorometric)
• Gene: CASP12

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Caspase-12 Assay Kit (Fluorometric)

• Apoptosis Related Cysteine Protease
• CASP 12
• CASP12
• CASP12P1
• Caspase 12 pseudogene 1
• Caspase12
• Caspase-12
• UNQ9415

Background

Caspase family of proteases are the central mediators of apoptosis in mammalian cells. The Caspase-12 Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (WLmax = 400 nm); upon cleavage of the substrate, free AFC emits a yellow-green fluorescence (WLmax = 505 nm), which can be quantified using a fluorometer or a fluorescence microplate reader.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CASP12