Catalase Activity Assay Kit (Colorimetric)

Catalase Activity Assay Kit (Colorimetric) [NBP3-24476] - Standard Reference Curve

Catalase Activity Assay Kit (Colorimetric) [NBP3-24476] - Assay Example

• Reactivity: All-NA
• Applications: Func
• Suitable Sample Type: Serum, plasma, cell samples, cell culture supernatant and tissue
• Standard Curve Range: 1.12 - 150 U/mL
• Sensitivity: 1.12 U/mL

Catalase Activity Assay Kit (Colorimetric) Summary

• Description: Detection instrument: Microplate reader (400 - 410 nm, optimum wavelength: 405 nm)
  Assay time: 40 min
• Standard Curve Range: 1.12 - 150 U/mL
• Sensitivity: 1.12 U/mL
• Inter-Assay: %CV < 7.7
• Intra-Assay: %CV < 3.9
• Spike Recovery: Average recovery rate: 100%
• Kit Type: Activity Assay Kit (Colorimetric)
• Gene: CAT

Applications/Dilutions

• Dilutions:
  • Functional

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Catalase Activity Assay Kit (Colorimetric)

• Cas1
• CAT
• Catalase
• Cs-1
• EC 1.11.1.6
• MGC138422
• MGC138424

Background

Human catalase is an heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen; it is implicated in ethanol metabolism, inflammation, apoptosis, aging and cancer. The 1.5A resolution human enzyme structure, both with and without bound NADPH, establishes the conserved features of mammalian catalase fold and assembly, implicates Tyr370 as the tyrosine radical, suggests the structural basis for redox-sensitive binding of cognate mRNA via the catalase NADPH binding site, and identifies an unexpectedly substantial number of water-mediated domain contacts (1). Because catalase has a low affinity for H2O2, it has been suggested that glutathione peroxidase clears most H2O2 within the erthrocyte and that catalase is of little import. It has been hypothesized that erythrocyte catalase might function to protect heterologous somatic cells against challenge by high levels of exogenous H2O2 (e.g., in areas of inflamation) (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CAT