Cathepsin L Antibody (193702)

• Reactivity: I
• Applications: WB, IP
• Clone: 193702
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cathepsin L Antibody (193702) Summary

• Immunogen: S. frugiperda ovarian cell line Sf 21-derived Insect Cathepsin L
• Specificity: Detects pro and mature forms of Insect Cathepsin L in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Cathepsin L is observed and no cross-reactivity with recombinant mouse Cathepsin L is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CTSL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin L Antibody (193702)

• Cathepsin L
• cathepsin L1
• CATL
• CTSL
• CTSL1
• CTSLEC 3.4.22.15
• EC 3.4.22
• FLJ31037
• Major excreted protein
• MEP

Background

The pro form of insect cell Cathepsin L is stable at neutral pH, but autoactivates in acidic buffers under reducing conditions. The activated enzyme is similar in substrate specificity to mammalian Cathepsin L, and is very active as a protease, as judged by its ability to degrade azocasein. Cystatins A, B, C, E/M, F, SA, and SN are potent inhibitors of the insect cell Cathepsin L. Like other cysteine proteases related to papain, insect cell Cathepsin L is also inhibited by E-64 and reagents which react with thiols.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin L Antibody (MAB22591)(16)

Publications using MAB22591

• Meigen Yu, Hui Ye, Ruth B. De-Paula, Carl Grant Mangleburg, Timothy Wu, Tom V. Lee et al. Functional screening of lysosomal storage disorder genes identifies modifiers of alpha-synuclein neurotoxicity PLOS Genetics 5/18/2023 [PMID: 37200393]
• Caitlin E. O’Brien, Susan H. Younger, Lily Yeh Jan, Yuh Nung Jan The GARP complex prevents sterol accumulation at the trans-Golgi network during dendrite remodeling Journal of Cell Biology 1/2/2023 [PMID: 36239632]
• L Wang, G Lin, Z Zuo, Y Li, SK Byeon, A Pandey, HJ Bellen Neuronal activity induces glucosylceramide that is secreted via exosomes for lysosomal degradation in glia Oncogene, 2022-07-13;8(28):eabn3326. 2022-07-13 [PMID: 35857503] (IHC, Drosophila melanogaster)
• X Tao, Y Zhu, Z Diaz-Perez, SH Yu, JR Foley, TM Stewart, RA Casero, R Steet, RG Zhai Phenylbutyrate modulates polyamine acetylase and ameliorates Snyder-Robinson syndrome in a Drosophila model and patient cells JCI Insight, 2022-07-08;7(13):. 2022-07-08 [PMID: 35801587] (IHC, Western Blot, Drosophila melanogaster)
• JR Florian, SJ DeMatte, DM Sweeder, RW Ordway, F Kawasaki Genetic analysis of the Drosophila ESCRT-III complex protein, VPS24, reveals a novel function in lysosome homeostasis PLoS ONE, 2021-05-06;16(5):e0251184. 5/6/2021 [PMID: 33956855]
• Torsten W.B. Götz, Dmytro Puchkov, Veronika Lysiuk, Janine Lützkendorf, Alexander G. Nikonenko, Christine Quentin et al. Rab2 regulates presynaptic precursor vesicle biogenesis at the trans-Golgi Journal of Cell Biology 5/3/2021 [PMID: 33822845]
• Torsten W.B. Götz, Dmytro Puchkov, Veronika Lysiuk, Janine Lützkendorf, Alexander G. Nikonenko, Christine Quentin et al. Rab2 regulates presynaptic precursor vesicle biogenesis at the trans-Golgi Journal of Cell Biology 2021-05-03 [PMID: 33822845] (Immunohistochemistry, Drosophila melanogaster)
• CG Mangleburg, T Wu, HK Yalamanchi, C Guo, YC Hsieh, DM Duong, EB Dammer, PL De Jager, NT Seyfried, Z Liu, JM Shulman Integrated analysis of the aging brain transcriptome and proteome in tauopathy Mol Neurodegener, 2020-09-29;15(1):56. 2020-09-29 [PMID: 32993812] (Western Blot, Drosophila)
• Hui Ye, Shamsideen A Ojelade, David Li-Kroeger, Zhongyuan Zuo, Liping Wang, Yarong Li et al. Retromer subunit, VPS29, regulates synaptic transmission and is required for endolysosomal function in the aging brain eLife 4/14/2020 [PMID: 32286230]
• AA Mondragon, A Yalonetska, AJ Ortega, Y Zhang, O Naranjo, J Elguero, WS Chung, K McCall Lysosomal Machinery Drives Extracellular Acidification to Direct Non-apoptotic Cell Death Cell Rep, 2019-04-02;27(1):11-19.e3. 2019-04-02 [PMID: 30943394] (IHC)
• M ?entürk, G Lin, Z Zuo, D Mao, E Watson, AG Mikos, HJ Bellen Ubiquilins regulate autophagic flux through mTOR signalling and lysosomal acidification Nat. Cell Biol., 2019-02-25;21(3):384-396. 2019-02-25 [PMID: 30804504] (Western Blot, Insect)
• D Mao, G Lin, B Tepe, Z Zuo, KL Tan, M Senturk, S Zhang, BR Arenkiel, M Sardiello, HJ Bellen VAMP associated proteins are required for autophagic and lysosomal degradation by promoting a PtdIns4P-mediated endosomal pathway Autophagy, 2019-02-20;0(0):. 2019-02-20 [PMID: 30741620] (IHC, Human)
• C Li, JM Brazill, S Liu, C Bello, Y Zhu, M Morimoto, L Cascio, R Pauly, Z Diaz-Perez, MCV Malicdan, H Wang, L Boccuto, CE Schwartz, WA Gahl, CF Boerkoel, RG Zhai Spermine synthase deficiency causes lysosomal dysfunction and oxidative stress in models of Snyder-Robinson syndrome Nat Commun, 2017-11-02;8(1):1257. 2017-11-02 [PMID: 29097652] (Western Blot, Drosophila)
• Medina Hasanagic, Eline van Meel, Shan Luan, Rajeev Aurora, Stuart Kornfeld, Joel C. Eissenberg The lysosomal enzyme receptor protein (LERP) is not essential, but is implicated in lysosomal function in Drosophila melanogaster Biology Open 10/15/2015 [PMID: 26405051]
• Luan S, Ilvarsonn A, Eissenberg J The unique GGA clathrin adaptor of Drosophila melanogaster is not essential. PLoS ONE, 2012-09-20;7(9):e45163. 2012-09-20 [PMID: 23028818] (Western Blot, Drosophila)
• Johnson GD, Jiang W Characterization of cathepsin L secreted by Sf21 insect cells. Arch. Biochem. Biophys., 2005-10-21;444(1):7-14. 2005-10-21 [PMID: 16274658] (Immunoprecipitation, Western Blot, Insect)

Bioinformatics

• Gene Symbol: CTSL1