Cathepsin L Antibody (33/2)

Western Blot: Cathepsin L Antibody (33/2) [NB100-1775] - Whole cell extract of Mv 1 Lu cultured mink lung was separated on SDS-PAGE and blotted with Monoclonal Anti-Cathepsin L. The antibody was developed with Goat Anti-Mouse IgG (Fab), AlkPhos Conjugate and NBT/BCIP substrate. Lane A: Antibody dilution 1:400. Lane B: Negative control (only secondary antibody).

• Reactivity: Hu, Mu, Rt, Md
• Applications: WB, ELISA, ICC/IF, IHC, MA
• Clone: 33/2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Cathepsin L Antibody (33/2) Summary

• Immunogen: Purified full length native protein (Human lung cancer line EPLC 32 M1).
• Epitope: Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule).
• Localization: Lysosomes
• Specificity: Reacts specifically with native and denatured forms of human cathepsin L and procathepsin L. Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule). The antibody may be used for the suppression of the malignant growth of myeloma cells. Cross-reactivity is observed with rat cathepsin L (strong), mouse procathepsin L (weak) and mink cathepsin and procathepsin L. Does not cross-react with human cathepsin types B, D, H and S, procathepsins B and D, rat cathepsin B, and mouse procathepsins B and D.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CTSL
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Microarray
  • Western Blot 1:200
• Application Notes: The antibody may be used for the suppression of the malignant growth of myeloma cells. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
• Control:

  Cathepsin L Overexpression Lysate

Reactivity Notes

Cross-reacts with Mouse procathepsin L (weak), Rat cathepsin L (strong), and Mink cathepsin and procathepsin L. Does not cross-react with human cathepsin types B, D, H and S, procathepsins B and D, rat cathepsin B, and mouse procathepsins B and D. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for Cathepsin L Antibody (33/2)

• Cathepsin L
• cathepsin L1
• CATL
• CTSL
• CTSL1
• CTSLEC 3.4.22.15
• EC 3.4.22
• FLJ31037
• Major excreted protein
• MEP

Background

Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumour invasion and metastasis. Normally located in lysosomes of almost all mammalian cells, they can be secreted from the cell under certain conditions. Cathepsin L is responsible for most of the intralysosomal breakdown of normal cells. Cathepsin L is secreted by numerous transformed cells in its inactive proform, and mRNA expression level seems to correlate with their metastatic potential.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin L Antibody (NB100-1775)(6)

Publications using NB100-1775

• Hossain MI, Marcus JM, Lee JH et al. Restoration of CTSD (cathepsin D) and lysosomal function in stroke is neuroprotective Autophagy 2020-05-25 [PMID: 32450052] (WB, Mouse)
• Capoccia BJ, Jin RU, Kong YY et al. The ubiquitin ligase Mindbomb 1 coordinates gastrointestinal secretory cell maturation J Clin Invest 2013-04-01 [PMID: 23478405] (ICC/IF, Human, Mouse)
• Garcia V, Kohen P, Maldonado C et al. Transient expression of progesterone receptor and cathepsin-l in human granulosa cells during the periovulatory period Fertility and Sterility 2012-03-12 [PMID: 22281037] (WB, Human)
• Zheng X, Chu F, Chou PM et al. Cathepsin L inhibition suppresses drug resistance in vitro and in vivo: a putative mechanism. Am J Physiol Cell Physiol 2009-01-01 [PMID: 18971393]
• Zheng, X et al. Senescence-initiated Reversal of Drug Resistance. Cancer Res 64: 1773-1780. 2004-03-01 [PMID: 14996739]
• Weber E et al. Monoclonal antibodies against cathepsin L procathepsin L of different species. Hybridoma 16:159-66. 1997-01-01 [PMID: 9145318]

Bioinformatics

• Gene Symbol: CTSL
• Entrez:
  • 1514(Human)
  • 13039(Mouse)
  • 25697(Rat)
• Uniprot:
  • P07711()