Recombinant Human Cathepsin L Protein

SDS-Page: Recombinant Human Cathepsin L Protein [NBP1-99623]

Recombinant Human Cathepsin L Protein [NBP1-99623] - Measurement of Recombinant Human Cathepsin L Protein activity by Cathepsin L Assay Kit (Fluorometric) (NBP2-54843)

Product Discontinued

Recombinant Human Cathepsin L Protein Summary

• Description: Recombinant bioactive protein for aa114-333 of Human Cathepsin L with N-terminal His tag. Molecular weight 24.2kDa.
  
  Store at -80C, stable for at least 6 months as supplied. Use as provided or dilute with 50% glycerol to 0.1 mg/ml. Aliquot immediately and store at -80C. Avoid freeze/thaw cycles.
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab81780)
• Details of Functionality: Recombinant Human Cathepsin L Protein has been activated through auto-catalytic activation of Procathepsin L at low pH. The enzyme is fully active as seen from its ability to cleave a fluorogenic substrate Z-Phe-Arg-AFC (NBP2-54843).
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CTSL
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity:> 2,000 mU/mg (1 U = digestion of 1 umole of Z-Phe-Arg-AFC substrate (K142-100) per minute at 37C in 50 mM Sodium Acetate, 100 mM NaCl, pH 5.5).
  
  This product is useful in inhibitor screening, kinetic studies and cleavage of substrates in vitro or in tissue culture.
• Theoretical MW: 24.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 25 mM Sodium Acetate (pH 5.5), 50 mM NaCl, 50% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Cathepsin L Protein

• Cathepsin L
• cathepsin L1
• CATL
• CTSL
• CTSL1
• CTSLEC 3.4.22.15
• EC 3.4.22
• FLJ31037
• Major excreted protein
• MEP

Background

Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CTSL