![Western Blot: CCDC109A Antibody [NBP2-76948] - Western blot analysis of MCU on different lysates using anti-MCU antibody at 1/500 dilution.Positive control:Lane1: A549 Lane3: mouse spleen tissueLane2: HL-60 Lane4: mouse brain tissue](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Western-Blot-NBP2-76948-img0001.jpg "Western Blot: CCDC109A Antibody [NBP2-76948] - Western blot analysis of MCU on different lysates using anti-MCU antibody at 1/500 dilution.Positive control:Lane1: A549 Lane3: mouse spleen tissueLane2: HL-60 Lane4: mouse brain tissue")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant protein within Human CCDC109A aa 51-225 / 351. (SwissProt: Q8NE86 Human; SwissProt: Q3UMR5 Mouse) |
| Localization | Mitochondrion. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MCU |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.2% BSA, 50% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CCDC109A Antibody (NBP2-76948)Find related products by research area.
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| Gene Symbol | MCU |
![Immunocytochemistry/Immunofluorescence: CCDC109A Antibody [NBP2-76948] - ICC staining MCU in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76948-img0004.jpg "Immunocytochemistry/Immunofluorescence: CCDC109A Antibody [NBP2-76948] - ICC staining MCU in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry: CCDC109A Antibody [NBP2-76948] - Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-MCU antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Immunohistochemistry-NBP2-76948-img0009.jpg "Immunohistochemistry: CCDC109A Antibody [NBP2-76948] - Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-MCU antibody. Counter stained with hematoxylin.")
![Flow Cytometry: CCDC109A Antibody [NBP2-76948] - Flow cytometric analysis of A549 cells with MCU antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Flow-Cytometry-NBP2-76948-img0010.jpg "Flow Cytometry: CCDC109A Antibody [NBP2-76948] - Flow cytometric analysis of A549 cells with MCU antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.")
![Immunocytochemistry/Immunofluorescence: CCDC109A Antibody [NBP2-76948] - ICC staining MCU in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76948-img0002.jpg "Immunocytochemistry/Immunofluorescence: CCDC109A Antibody [NBP2-76948] - ICC staining MCU in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: CCDC109A Antibody [NBP2-76948] - ICC staining MCU in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76948-img0003.jpg "Immunocytochemistry/Immunofluorescence: CCDC109A Antibody [NBP2-76948] - ICC staining MCU in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry: CCDC109A Antibody [NBP2-76948] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-MCU antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Immunohistochemistry-NBP2-76948-img0005.jpg "Immunohistochemistry: CCDC109A Antibody [NBP2-76948] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-MCU antibody. Counter stained with hematoxylin.")
![Immunohistochemistry: CCDC109A Antibody [NBP2-76948] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MCU antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Immunohistochemistry-NBP2-76948-img0006.jpg "Immunohistochemistry: CCDC109A Antibody [NBP2-76948] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MCU antibody. Counter stained with hematoxylin.")
![Immunohistochemistry: CCDC109A Antibody [NBP2-76948] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MCU antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Immunohistochemistry-NBP2-76948-img0007.jpg "Immunohistochemistry: CCDC109A Antibody [NBP2-76948] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MCU antibody. Counter stained with hematoxylin.")
![Immunohistochemistry: CCDC109A Antibody [NBP2-76948] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MCU antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CCDC109A-Antibody-Immunohistochemistry-NBP2-76948-img0008.jpg "Immunohistochemistry: CCDC109A Antibody [NBP2-76948] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MCU antibody. Counter stained with hematoxylin.")
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Intracellular Staining by Flow Cytometry Reg1A Antibody (431202) [Unconjugated]](https://images.novusbio.com/images2/Reg1A_MAB4937_Flow_Cytometry_8512.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")