Recombinant Mouse CCL2/MCP1 His Protein

SDS-Page: Recombinant Mouse CCL2/MCP1 Protein [NBP2-22772]

• Reactivity: Mu
• Applications: Func, PAGE
• Concentration: 0.25 mg/ml

Recombinant Mouse CCL2/MCP1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 24-148 of Mouse His-tag CCL2/MCP1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQPDAVNAPL TCCYSFTSKM IPMSRLESYK RITSSRCPKE AVVFVTKLKR EVCADPKKEW VQTYIKNLDR NQMRSEPTTL FKTASALRSS APLNVKLTRK SEANASTTFS TTTSSTSVGV TSVTVN

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CCL2
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • In vivo assay
  • SDS-Page
• Application Notes: Use in In vivo reported in scientific literature (PMID:33510218)
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Mouse CCL2/MCP1 His Protein

• C-C motif chemokine 2
• chemokine (C-C motif) ligand 2
• GDCF-2
• HC11Monocyte secretory protein JE
• MCAFMonocyte chemotactic and activating factor
• MCP1 Monocyte chemotactic protein 1
• MCP-1 Small-inducible cytokine A2
• MGC9434
• Monocyte chemoattractant protein 1
• monocyte chemoattractant protein-1
• SCYA2HSMCR30
• small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je)
• small inducible cytokine subfamily A (Cys-Cys), member 2
• SMC-CF

Background

Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). CCL2 recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. Recombinant mouse CCL2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for CCL2/MCP1 Recombinant Protein (NBP2-22772)(1)

Publication using NBP2-22772

• Uddin MI, Kilburn TC, Jamal SZ, et al. A novel method for visualizing and tracking endogenous mRNA in a specific cell population in pathological neovascularization Scientific reports 2021-01-28 [PMID: 33510218] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: CCL2