CD161 Antibody (14F1F11) - Azide and BSA Free

Western Blot: CD161 Antibody (14F1F11) - Azide Free [NBP2-31359] - Analysis of a partial CD161 recombinant protein using CD161 antibody at 1 ug/ml.

Immunohistochemistry-Paraffin: CD161 Antibody (14F1F11) - Azide Free [NBP2-31359] - Analysis of CD161 in human kidney using CD161 antibody at 5 ug/ml.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 14F1F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

CD161 Antibody (14F1F11) - Azide and BSA Free Summary

• Immunogen: Amino acids 68-225 of human CD161 were used as the immunogen for this antibody.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLRB1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:2000
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 - 5ug/ml

Reactivity Notes

The antibody has not been tested in species samples other than human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CD161 Antibody (14F1F11) - Azide and BSA Free

• CD161 antigen
• CD161
• CLEC5B
• CLEC5BC-type lectin domain family 5 member B
• HNKR-P1a
• killer cell lectin-like receptor subfamily B member 1
• killer cell lectin-like receptor subfamily B, member 1
• KLRB1
• Ly59
• Natural killer cell surface protein P1A
• NKR
• NKRP1
• NKR-P1
• NKRP1A
• NKR-P1AMGC138614
• NKRP1ANKR

Background

CD161 is a C-Type Lectin Receptor, also known as NKR-P1A, previously found on NKT cells and subsets of T-cells. Phenotyping of cells that are functionally producing IL-17 has shown the one constant marker is CD161. While it had been shown previously that human CD4+ cells that produce both IL-17 and IFNg give rise to Th17/Th1 cells, indicating common etiology of these two T cell subsets, a clear marker for cells producing IL-17 is CD161. In fact, all cells characterized as the following subsets CD4+TCRab+, CD8+TCRab+, CD4-CD8-TCRab+, and CD4-CD8-TCRgd+ bearing CD161 also produce IL-17 but cells of these same phenotypes and functional marker which do not bear CD161 also do not produce IL-17. Induction of CD161 expression by transduction with RORC also induces IL-1R and IL-23R as well as the ability to produce IL-17. The ability to produce IL-17 and the induction of CD161 shows a strong dependency for RORC. Antibody to CD161 provides a probe for identifying IL-17 producing cells and offers a finer delineation of additional subsets derived from Th17 and their functional characteristics through cytokine mRNA expression or production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLRB1