CD163 Antibody (EDHu-1) - BSA Free

Images

 
CD163 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human CD163 Monoclonal Antibody (Novus Catalog # NB110-40686) at 1:50 at 37 °Celsius for 8 minutes. Before incubation ...read more
CD163 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD163 Monoclonal Antibody (Novus Catalog # NB110-40686) at 20 µg/mL at 37 ° Celsius for 8 minutes. Before ...read more
CD163 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CD163 Monoclonal Antibody (Novus Catalog # NB110-40686) at 1:50 at 37 °Celsius for 8 minutes. Before incubation ...read more
Non-repaired and repaired VML injured muscles present sustained putative macrophage infiltration. Representative histologic images labeled with CD163 (pan macrophage marker, green) and wheat germ agglutinin (WGA, red) ...read more
A surface stain was performed on hPBMCs with CD163 (EDHu-1) antibody NB110-40686AF700 and a matched isotype control NBP2-27287AF700. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room ...read more
Staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1.
A surface stain was performed on human peripheral blood monocytes with CD163 (EDHu-1) antibody NB110-40686APC and a matched isotype control NBP2-27287APC. Cells were incubated in an antibody dilution of 1 ug/mL for 20 ...read more
FFPE human tonsil, stained with anti-CD163, pH 9 antigen retrieval. IHC-P Image submitted by a verified customer review.
A surface stain was performed on hPBMCs with CD163 (EDHu-1) antibody NB110-40686PE and a matched isotype control NBP2-27287PE. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room ...read more
Human tonsil cryosection with Mouse anti Human CD163 antibody, clone, red in A and Mouse anti Human CD21 antibody, green in B. C is the merged image with nuclei counterstained blue using DAPI.
Frozen human skin sections. CD163 (green) in control human skin. Sections were fixed with 4% PFA PBS, permeabilized wtih 0.5% Triton TBS Ca/Azide, and blocked with a 5% BSA 0.05% T20 TBS Ca/Azide solution. Antibody was ...read more
Staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1.
Immunohistochemistry-Frozen: CD163 Antibody (EDHu-1) [NB110-40686] - Staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1.
Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in a formalin fixed paraffin embedded section of canine lymph node. NB110-40686 was diluted to 0.25ug per mL ...read more
Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in an FFPE section of human tonsil. NB110-40686 was diluted to 250 ng/mL and left on tissue sections for ...read more
Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in an FFPE section of equine spleen. NB110-40686 was diluted to 250ng per mL and left on tissue sections for ...read more
Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in an FFPE section of feline lymph node. NB110-40686 was diluted to 250ng per mL and left on tissue sections ...read more
Immunocytochemistry/ Immunofluorescence: CD163 Antibody (EDHu-1) [NB110-40686] - Non-repaired & repaired VML injured muscles present sustained putative macrophage infiltration. Representative histologic images labeled ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, mIF
Clone
EDHu-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

CD163 Antibody (EDHu-1) - BSA Free Summary

Immunogen
Leucocytes harvested from the pleural cavity of patients with idiopathic spontaneous pneumothorax
Marker
Histiocytic Marker
Specificity
NB110-40686 recognizes the human CD163 cell surface antigen, a 130-140 kDa glycoprotein expressed by tissue macrophages. CD163 is not expressed by resting peripheral blood leucocytes, but expression may be induced on monocytes by culture in dexamethasone.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD163
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Flow (Cell Surface) 1:10 - 1:1000
  • Flow Cytometry 1:10 - 1:1000
  • Immunoassay
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Multiplex Immunofluorescence
  • Western Blot 1:100 - 1:2000
Application Notes
Clone EDHu-1 is reported to inhibit the binding of haptoglobin:haemoglobin complexes to CD163, see Madsen, M. et al. for details. Removal of sodium azide is recommended prior to use in functional studies.
Theoretical MW
125.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CD163 Overexpression Lysate
Reviewed Applications
Read 8 Reviews rated 4.8
using
NB110-40686 in the following applications:

Publications
Read Publications using
NB110-40686 in the following applications:

Reactivity Notes

Predicted cross-reactivities: Sheep, Rhesus Monkey, Guinea Pig, Bovine, Cynomolgus monkey, Porcine

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CD163 Antibody (EDHu-1) - BSA Free

  • CD_antigen: CD163
  • CD163 molecule
  • CD163
  • GHI/61
  • HbSR
  • Hemoglobin scavenger receptor
  • M130
  • macrophage-associated antigen
  • MM130
  • RM3/1
  • SCARI1
  • scavenger receptor cysteine-rich type 1 protein M130
  • sCD163
  • Soluble CD163

Background

CD163 (Cluster of Differentiation 163), also known by several other names including M130, p155, RM3/1, Ki-M8, Ber-MAC3, SM4, and GHI/61, is a type I transmembrane glycoprotein that is a member of the scavenger receptor cysteine-rich (SRCR) super family class B (1-3). CD163 is expressed specifically on the monocyte/macrophage lineage and has a theoretical molecular weight of 130-160 kDa in reducing conditions and 110 kDa in non-reducing conditions (2 - 6). CD163 is synthesized as 1076 amino acid (aa) protein consisting of a large extracellular domain (ECD) with nine SRCR domains, proline-serine-threonine rich (PST) linker domain, a transmembrane segment, and a cytoplasmic tail which has varying lengths depending on the isoform due to alternative splicing, with the short 49 aa form being the most common (1-3, 6).

One of the primary functions of CD163 is uptake of haptoglobin-hemoglobin (Hp-Hb) complexes from the liver, spleen, and bone marrow, ultimately triggering an anti-inflammatory response (3, 5, 7). CD163 also functions as an erythroblast adhesion receptor and promotes cell maturation and survival (3, 5, 7). Furthermore, CD163 functions in immune sensing of bacteria and as a receptor for tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) (3, 5, 7). As mentioned above, CD163 is expressed on cells in the monocyte/macrophage lineage and, in general, anti-inflammatory signals including glucocorticoids, interleukin (IL)-6, and IL-10 stimulate CD163 synthesis and expression while, conversely, pro-inflammatory signals such as interferon-gamma (INF-gamma), TNF-alpha, and lipopolysaccharide (LPS) downregulate CD163 (3, 5). In addition to membrane-bound form of CD163, the protein can be cleaved by metalloproteinases (MMP) and induced by LPS or phorbol myristate acetate (PMA) to release a soluble form (sCD163) into the plasma (7). Increased levels of sCD163 in the plasma and an increased number of CD163-expressing macrophages at the site of inflammation are associated with a variety of pathologies (3, 5-7). CD163/sCD163 is often increased and a suitable clinical marker for inflammatory diseases including rheumatoid arthritis (RA), Gaucher disease, chronic kidney disease, diabetes, and Crohn's disease (3, 5-7).

Alternative names for CD163 includes GHI/61, HbSR, Hemoglobin scavenger receptor, M130, macrophage-associated antigen, MM130, RM3/1, SCARI1, scavenger receptor cysteine-rich type 1 protein M130, sCD163, and soluble CD163.

References

1. Law, S. K., Micklem, K. J., Shaw, J. M., Zhang, X. P., Dong, Y., Willis, A. C., & Mason, D. Y. (1993). A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily. European journal of immunology. https://doi.org/10.1002/eji.1830230940

2. Onofre, G., Kolackova, M., Jankovicova, K., & Krejsek, J. (2009). Scavenger receptor CD163 and its biological functions. Acta medica (Hradec Kralove).

3. Van Gorp, H., Delputte, P. L., & Nauwynck, H. J. (2010). Scavenger receptor CD163, a Jack-of-all-trades and potential target for cell-directed therapy. Molecular immunology. https://doi.org/10.1016/j.molimm.2010.02.008

4. Sulahian, T. H., Hogger, P., Wahner, A. E., Wardwell, K., Goulding, N. J., Sorg, C., Droste, A., Stehling, M., Wallace, P. K., Morganelli, P. M., & Guyre, P. M. (2000). Human monocytes express CD163, which is upregulated by IL-10 and identical to p155. Cytokine. https://doi.org/10.1006/cyto.2000.0720

5. Etzerodt, A., & Moestrup, S. K. (2013). CD163 and inflammation: biological, diagnostic, and therapeutic aspects. Antioxidants & redox signaling. https://doi.org/10.1089/ars.2012.4834

6. Skytthe, M. K., Graversen, J. H., & Moestrup, S. K. (2020). Targeting of CD163+ Macrophages in Inflammatory and Malignant Diseases. International journal of molecular sciences, 21(15), 5497. https://doi.org/10.3390/ijms21155497

7. Moller H. J. (2012). Soluble CD163. Scandinavian journal of clinical and laboratory investigation. https://doi.org/10.3109/00365513.2011.626868

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD163 Antibody (NB110-40686)(25)

We have publications tested in 6 confirmed species: Human, Bovine, Macaque, Monkey, Porcine, Rhesus Macaque.

We have publications tested in 8 applications: B/N, ELISA, FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P.


Filter By Application
B/N
(1)
ELISA
(1)
FLOW
(3)
ICC/IF
(3)
IF/IHC
(6)
IHC
(1)
IHC-Fr
(1)
IHC-P
(5)
All Applications
Filter By Species
Human
(6)
Bovine
(3)
Macaque
(1)
Monkey
(1)
Porcine
(3)
Rhesus Macaque
(2)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB110-40686 Applications Species
Gupta T, Antanaviciute A, Hyun-Jung Lee C, Ottakandathil Babu R et Al. Tracking in situ checkpoint inhibitor-bound target T cells in patients with checkpoint-induced colitis Cancer Cell 2024-05-14 [PMID: 38744246]
Koenig MR, Mitzey AM, Morgan TK et al. Infection of the maternal-fetal interface and vertical transmission following low-dose inoculation of pregnant rhesus macaques (Macaca mulatta) with an African-lineage Zika virus PloS one 2023-05-04 [PMID: 37141276] (IHC, Macaque)

Details:
Macaca mulatta, Rhesus Macagues
IHC Macaque
Scheepstra KWF, Mizee MR, van Scheppingen J et al. Microglia transcriptional profiling in major depressive disorder shows inhibition of cortical grey matter microglia Biological psychiatry 2023-04-28 [PMID: 37121366] (IHC-P) IHC-P
Olwenyi O, Johnson S, Bidokhti M et al. Chronic Morphine Exposure distorts Gut-Brain Interrelationships in SHIV Infected Rhesus Macaques Research Square 2022-03-02 (ICC/IF, Rhesus Macaque) ICC/IF Rhesus Macaque
Maldonado LAG, Nascimento CR, Rodrigues Fernandes NA et al. Influence of tumor cell-derived TGF-beta on macrophage phenotype and macrophage-mediated tumor cell invasion The international journal of biochemistry & cell biology 2022-12-01 [PMID: 36343916] (IHC-P, Human) IHC-P Human
Badr Y, Noreldin AE, Elewa YHA et al. Cellular infiltration, cytokines, and histopathology of skin lesions associated with different clinical forms and stages of naturally occurring lumpy skin disease in cattle Comparative immunology, microbiology and infectious diseases 2022-10-07 [PMID: 36270050] (IHC-P, Bovine) IHC-P Bovine
Ding M, Dang W, Liu H et al. Combinational Deletions of MGF360-9L and MGF505-7R Attenuated Highly Virulent African Swine Fever Virus and Conferred Protection against Homologous Challenge Journal of virology 2022-07-27 [PMID: 35867564] (ICC/IF, Bovine) ICC/IF Bovine
Monguio-Tortajada M, Prat-Vidal C, Martinez-Falguera D et al. Acellular cardiac scaffolds enriched with MSC-derived extracellular vesicles limit ventricular remodelling and exert local and systemic immunomodulation in a myocardial infarction porcine model Theranostics 2022-06-06 [PMID: 35832072] (FLOW, IHC-Fr, Porcine) FLOW, IHC-Fr Porcine
Fujita M, Matsumoto T, Hayashi S et al. Paracrine effect of the stromal vascular fraction containing M2 macrophages on human chondrocytes through the Smad2/3 signaling pathway Journal of cellular physiology 2022-06-29 [PMID: 35766589]
Jiang S, Chan CN, Rovira-ClavE X et al. Combined protein and nucleic acid imaging reveals virus-dependent B cell and macrophage immunosuppression of tissue microenvironments Immunity 2022-04-12 [PMID: 35447093]
Show All 25 Publications.

Reviews for CD163 Antibody (NB110-40686) (8) 4.88

Average Rating: 4.8
(Based on 8 reviews)
We have 8 reviews tested in 6 species: Human, Rat, Cat, Dog, Horse, Rabbit.

Reviews using NB110-40686:
Filter by Applications
IHC-P
(5)
ELISA
(1)
IHC-Fr
(1)
Immunofluorescence - paraffin
(1)
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Human
(3)
Rat
(1)
Cat
(1)
Dog
(1)
Horse
(1)
Rabbit
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD163 NB110-40686
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5
reviewed by:
Verified Customer
IHC-P Human 04/23/2024
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedTonsil
SpeciesHuman
Lot161497

Comments

CommentsSection underwent head induced epitope retrieval for 20min in a vegetable steamer using Target Retrieval Solution.
Immunohistochemistry-Paraffin CD163 NB110-40686
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5
reviewed by:
Verified Customer
IHC-P Horse 04/23/2024
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSpleen
SpeciesHorse
Lot161497

Comments

Comments***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.*** Section underwent head induced epitope retrieval for 20min in a vegetable steamer using Target Retrieval Solution.
Immunohistochemistry-Paraffin CD163 NB110-40686
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5
reviewed by:
Verified Customer
IHC-P Cat 04/23/2024
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedLymph node
SpeciesCat
Lot161497

Comments

Comments***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.*** Section underwent head induced epitope retrieval for 20min in a vegetable steamer using Target Retrieval Solution
Immunohistochemistry-Paraffin CD163 NB110-40686
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5
reviewed by:
Verified Customer
IHC-P Dog 12/07/2023
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedLymph node
SpeciesDog
Lot161497

Comments

Comments***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.*** Section underwent 20m of heat induced epitope retrieval in a vegetable steamer using a citrate buffer. No signal was detected in sections that did not undergo HIER.
ELISA CD163 NB110-40686
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4
reviewed by:
Verified Customer
ELISA Rat 04/28/2021
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Summary

ApplicationELISA
Sample TestedRat inner ear
SpeciesRat
Lot149022B

Comments

Comments***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***
Immunohistochemistry-Frozen CD163 NB110-40686
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5
reviewed by:
Tyler Crowe
IHC-Fr Human 03/10/2020
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen human skin sections
SpeciesHuman
Lot149022A

Comments

CommentsSections were fixed with 4% PFA PBS, permeabilized wtih 0.5% Triton TBS Ca/Azide, and blocked with a 5% BSA 0.05% T20 TBS Ca/Azide solution. Antibody was diluted 1:100 and applied for 1 hr at 37C. Detection was performed with anti-host conjugated antibody, and sections were counterstained with DAPI.
Other CD163 NB110-40686
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5
reviewed by:
Verified Customer
Immunofluorescence - paraffin Human 11/28/2018
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Summary

ApplicationOther
Sample Testedhuman tonsil
SpeciesHuman
LotI don't have it any more

Comments

CommentspH 9 antigen retrieval
Immunohistochemistry-Paraffin CD163 NB110-40686
Enlarge
4
reviewed by:
seulgee lee
IHC-P Rabbit 04/24/2018
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedvessel
SpeciesRabbit
Lot1853

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Recent Reviews

4.8
5
6
4
2
3
0
2
0
1
0

Verified Customer
04/23/2024
Application: IHC-P
Species: Human

Verified Customer
04/23/2024
Application: IHC-P
Species: Horse

Verified Customer
04/23/2024
Application: IHC-P
Species: Cat

Bioinformatics

Gene Symbol CD163
Entrez
Uniprot