CD3 gamma Antibody - BSA Free

Western Blot: CD3 gamma Antibody [NBP1-31250] - Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE, antibody diluted at 1:3000.

Immunohistochemistry-Paraffin: CD3 gamma Antibody [NBP1-31250] - Human PANC1 xenograft. CD3 gamma antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Flow Cytometry: CD3 gamma Antibody [NBP1-31250] - Sample: mouse splenocytes cell fixed in 4% paraformaldehyde at 4 degrees C for 15 min. Black: Unlabelled sample was used as a control. Red: CD3 gamma antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

• Reactivity: Hu, Po, RM
• Applications: WB, Flow, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CD3 gamma Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD3 gamma. The exact sequence is proprietary.
• Localization: Membrane
• Specificity: This antibody is specific to the human CD3G protein.
• Predicted Species: Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CD3G
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:200
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CD3 gamma Antibody - BSA Free

• CD3 gamma
• CD3g antigen
• CD3g antigen, gamma polypeptide (TiT3 complex)
• CD3g molecule, epsilon (CD3-TCR complex)
• CD3g molecule, gamma (CD3-TCR complex)
• CD3g
• CD3-GAMMA
• FLJ17620
• FLJ17664
• FLJ79544
• FLJ94613
• IMD17
• MGC138597
• T3G
• T-cell antigen receptor complex, gamma subunit of T3
• T-cell receptor T3 gamma chain
• T-cell surface glycoprotein CD3 gamma chain

Background

The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CD3G