CD44 Antibody - BSA Free

Western Blot: CD44 Antibody [NBP1-31488] - Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody.

Western Blot: CD44 Antibody [NBP1-31488] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody diluted at 1:7000. Multiple bands were observed 130-180 kDa. It is possibly due to alternative splicing.

Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody. The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.

Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody. The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.

Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP1-31488] - Analysis of methanol-fixed HeLa, using CD44 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Human cervical carcinoma. CD44 stained by CD44 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Human endometrial carcinoma. CD44 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Human pancreatic tumor, using CD44 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunocytochemistry/ Immunofluorescence: CD44 Antibody [NBP1-31488] - CD44 antibody detects CD44 protein at cell membrane by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: CD44 stained by CD44 antibody (NBP1-31488) diluted at 1:500.Blue: Fluoroshield with DAPI .

Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody (NBP1-31488) diluted at 1:45000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: CD44 Antibody [NBP1-31488] - Non-transfected (–) and transfected (+) Wild-type (WT) and G CD44 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: CD44 Antibody [NBP1-31488] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Rt, Rb
• Applications: WB, Func, ICC/IF, IHC, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CD44 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human CD44. The exact sequence is proprietary.
• Localization: Membrane |
• Marker: Cell Membrane Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CD44
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen Reported in scientific literature (PMID: 25362854)
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation Assay dependent
  • In vitro assay Reactivity reported in scientific literature (PMID: 32976824)
  • Knockout Validated
  • Western Blot 1:1000-1:20000
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (82%). Mouse reactivity reported in (PMID: 25362854).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for CD44 Antibody - BSA Free

• CD44 antigen
• CD44 molecule (Indian blood group)
• CD44
• CD44R
• CDw44
• cell surface glycoprotein CD44
• chondroitin sulfate proteoglycan 8
• CSPG8
• ECMR-III
• epican
• Extracellular matrix receptor III
• GP90 lymphocyte homing/adhesion receptor
• HCAM
• HCELL
• hematopoietic cell E- and L-selectin ligand
• Heparan sulfate proteoglycan
• Hermes antigen
• homing function and Indian blood group system
• HUTCH-I
• Hyaluronate receptor
• IN
• LHR
• MC56
• MDU2
• MDU2CD44 antigen (homing function and Indian blood group system)
• MDU3
• MDU3CDW44
• MIC4
• MIC4MGC10468
• MUTCH-I
• Pgp1
• PGP-1
• PGP-I
• Phagocytic glycoprotein 1
• Phagocytic glycoprotein I

Background

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD44 Antibody (NBP1-31488)(13)

Publications using NBP1-31488

• Yoon C, Lu J, Jun Y et al. KRAS activation in gastric cancer stem-like cells promotes tumor angiogenesis and metastasis Research Square 2022-12-06 (IHC-P, Mouse, Human)
• Yoon C, Lu J, Jun Y et al. KRAS activation in gastric cancer stem-like cells promotes tumor angiogenesis and metastasis BMC cancer 2023-07-22 [PMID: 37481516] (IHC-P, Human)
• Mohanty S, Mohapatra P, Shriwas O et al. CRISPR-based kinome-screening revealed MINK1 as a druggable player to rewire 5FU-resistance in OSCC through AKT/MDM2/p53 axis bioRxiv 2021-08-20 [PMID: 36182968]
• Lubanska D, Alrashed S, Mason GT et al. Impairing proliferation of glioblastoma multiforme with CD44+ selective conjugated polymer nanoparticles Scientific reports 2022-07-15 [PMID: 35840697] (ICC/IF)
• Kainulainen K, Takabe P, Heikkinen S et al. M1 macrophages induce pro-tumor inflammation in melanoma cells via TNFR-NF-kappa The Journal of investigative dermatology 2022-05-14 [PMID: 35580697] (ICC/IF, Human)
• Patel U, Kannan S, Rane SU et al. Prognostic and predictive roles of cancer stem cell markers in head and neck squamous cell carcinoma patients receiving chemoradiotherapy with or without nimotuzumab British journal of cancer 2022-02-09 [PMID: 35140342] (IF/IHC, Human)
• Lu J, Cao LL, Xu Y et al. FOXC1 modulates stem-like cell properties and chemoresistance through Hedgehog and EMT signaling in gastric adenocarcinoma Molecular therapy : the journal of the American Society of Gene Therapy 2021-09-14 [PMID: 34534693]
• Mohapatra P, Shriwas O, Mohanty S, et al. CMTM6 drives cisplatin resistance by regulating Wnt signaling through ENO-1/AKT/GSK3 beta axis JCI insight 2021-01-12 [PMID: 33434185] (IF/IHC, Human)
• D'Ascola A, Scuruchi M, Ruggeri RM et al. Hyaluronan oligosaccharides modulate inflammatory response, NIS and thyreoglobulin expression in human thyrocytes Arch. Biochem. Biophys. 2020-09-22 [PMID: 32976824] (WB, In vitro, Human)
• Shriwas O, Priyadarshini M, Samal SK et al. DDX3 modulates cisplatin resistance in OSCC through ALKBH5-mediated m6A-demethylation of FOXM1 and NANOG Apoptosis 2020-01-23 [PMID: 31974865]
• You, K, Parikh, P Et al. Moderate hyperoxia induces senescence in developing human lung fibroblasts. Am J Physiol Lung Cell Mol Physiol 2019-11-01 [PMID: 31411059] (IF/IHC, Mouse)
• Liang Y, Gao H, Lin Sy et al. Mcph1/Brit1 deficiency promotes genomic instability and tumor formation in a mouse model Oncogene et al. 2014-11-03 [PMID: 25362854] (IHC-Fr, Mouse)
  
  Details:
  CD25/IL-2 R alpha antibody used for IHC-Fr on thymus tissues from wild-type (WT) mice and primary lymphomas from Mcph1+/+p53-/-, Mcph1+/-p53-/-, and Mcph1-/-p53-/- mice. The antibody was used as the molecular marker for lymphocyte development and normal thymus was considered as control (Figure S8)
• Alberton P, Dex S, Popov C et al. Loss of tenomodulin results in reduced self-renewal and augmented senescence of tendon stem/progenitor cells. Stem Cells Dev. 2014-10-28 [PMID: 25351164]

Bioinformatics

• Gene Symbol: CD44
• Entrez:
  • 960(Human)
• Uniprot:
  • P16070()