Cdk9 Antibody - BSA Free

Western Blot: Cdk9 Antibody [NB120-10874] - 1) PC3 and 2) DU145 prostate cancer cells (50ug per lane). Bands at the expected MW of 55 and 42 Kda were detected. Personal communication Flavio Rizzolio, Temple University

Immunocytochemistry/Immunofluorescence: Cdk9 Antibody [NB120-10874] - Immunocytochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The staining shows the location of mcdk9/PITALRE protein in developing mouse tissue. Arrows indicate areas of high expression. Panel A: Peroxidase-DAB immunostaining of mcdk9/PITALRE protein in the developing mouse brain in the differentiated region of the medulla oblongata just below the fourth ventricle. Similar staining is shown in Panel B in the dorsal root ganglia. Panel C: Fluorescein immunofluorescence of mcdk9IPITALRE in skeletal muscle. Similar staining is shown in Panel D in cardiac muscle. Other detection systems should yield similar results.

Western Blot Results of Anti-Cdk9 Antibody. Lane 1: Opal Prestined Molecular Weight Marker

• Reactivity: Hu, Mu
• Applications: WB, DB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cdk9 Antibody - BSA Free Summary

• Description: This product was prepared from monospecific antiserum by delipidation and defibrination
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene Cdk9 (PITALRE). (Uniprot: P50750)
• Localization: Nuclear
• Specificity: No reaction was observed against other related cyclin dependent kinases.
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDK9
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:10000-1:50000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:3000
• Application Notes: This antibody has been tested for use in ELISA, immunoprecipitation, immunocytochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 43 kDa corresponding to CDK9 (PITALRE) by western blotting in the appropriate cell lysate or extract. HeLa cells may be used as a positive control.

Reactivity Notes

Antiserum will specifically react with a 43 kDa Cdk9 (PITALRE) protein from human, rat and mouse tissue. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with Cdk9 (PITALRE) from other species may also occur. The murine cDNA is shown to be 98% identical with human. For immunohistochemistry use paraffin embedded tissue.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Delipidation and Defibrination

Alternate Names for Cdk9 Antibody - BSA Free

• C-2K
• CDC2L4
• CDC2L4CTK1
• CDC2-related kinase
• Cdk9
• Cell division cycle 2-like protein kinase 4
• Cell division protein kinase 9
• cyclin-dependent kinase 9 (CDC2-related kinase)
• cyclin-dependent kinase 9
• EC 2.7.11
• EC 2.7.11.22
• EC 2.7.11.23
• PITALRE
• serine/threonine protein kinase PITALRE
• Serine/threonine-protein kinase PITALRE
• TAK

Background

CDK9 (PITALRE) (also known as cyclin-dependent kinase 9, Serine/threonine-protein kinase PITALRE, C-2K and Cell division cycle 2-like protein kinase 4) is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. CDK9 (PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. This kinase also was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. CDK9 (PITALRE) is a ubiquitously expressed nuclear protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDK9
• Entrez:
  • 1025(Human)
  • 107951(Mouse)
  • 362110(Rat)
• Uniprot:
  • P50750(Human)