CDX2 Antibody - BSA Free

Immunohistochemistry-Paraffin: CDX2 Antibody [NB100-2136] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CDX2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: CDX2 Antibody [NB100-2136] - Detection of Human CDX2 on HeLa whole cell lysate using NB100-2136. CDX2 was also immunoprecipitated by rabbit anti-CDX2 antibody NB100-2137 using 3 mcg/mg lysate.

• Reactivity: Hu, Mu
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CDX2 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 250 and 300 of human Caudal Type Homeobox Transcription Factor 2 using the numbering given in entry NP_001256.1 (GeneID 1045).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDX2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 1-4 ug/mg lysate
• Application Notes: Western Blot Not recommended. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Control:

  CDX2 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:33207226).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDX2 Antibody - BSA Free

• caudal type homeo box transcription factor 2
• caudal type homeobox 2
• Caudal-type homeobox protein 2
• CDX2
• CDX3
• CDX-3
• CDX3caudal type homeobox transcription factor 2
• homeobox protein CDX-2

Background

CDX2 is a caudal type homeobox gene that encodes an intestine-specific transcription factor that is expressed early in intestinal development and may be involved in the regulation of proliferation and differentiation of intestinal epithelial cells. It is expressed in the nuclei of epithelial cells throughout the intestine, from duodenum to rectum. The CDX2 protein is expressed in primary and metastatic colorectal carcinomas and has also been demonstrated in the intestinal metaplasia of the stomach and intestinal-type gastric cancer, while it is not expressed in the normal gastric mucosa. Studies have shown that CDX2 is superior marker compared to CK20.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDX2 Antibody (NB100-2136)(12)

Publications using NB100-2136

• Mena-Osuna R, Mantrana A, Guil-Luna S et al. Metabolic shift underlies tumor progression and immune evasion in S-nitrosoglutathione reductase-deficient cancer The Journal of pathology 2023-04-05 [PMID: 37017456] (IHC, Human)
• Mochizuki K, Kudo SE, Kato K et al. Molecular and clinicopathological differences between depressed and protruded T2 colorectal cancer PloS one 2022-10-20 [PMID: 36264865] (IF/IHC, Human)
• Adhikari D, Lee IW, Al-Zubaidi U et al. Depletion of oocyte dynamin-related protein 1 shows maternal-effect abnormalities in embryonic development Science advances 2022-06-17 [PMID: 35704569] (ICC/IF, Mouse)
• Kasashima H, Duran A, Martinez-OrdoNez A et al. Stromal SOX2 Upregulation Promotes Tumorigenesis through the Generation of a SFRP1/2-Expressing Cancer-Associated Fibroblast Population Dev Cell 2020-11-10 [PMID: 33207226] (IF/IHC, Mouse)
• Li Y, Yao Q, Zhang L et al. Immunohistochemistry-Based Consensus Molecular Subtypes as a Prognostic and Predictive Biomarker for Adjuvant Chemotherapy in Patients with Stage II Colorectal Cancer Oncologist 2020-09-14 [PMID: 32926498] (MiAr, Human)
• Yao Y, Xu X, Yang L et al. Patient-Derived Organoids Predict Chemoradiation Responses of Locally Advanced Rectal Cancer Cell Stem Cell 2019-11-19 [PMID: 31761724] (IF/IHC, Human)
• Sandberg TP, Sweere I, van Pelt GW et al. Prognostic value of low CDX2 expression in colorectal cancers with a high stromal content - a short report Cell Oncol (Dordr) 2019-03-08 [PMID: 30847807] (IF/IHC, Human)
• Trinh A, Trumpi K, de Sousa E Melo F et al. Practical and Robust Identification of Molecular Subtypes in Colorectal Cancer by Immunohistochemistry. Clin Cancer Res 2016-07-26 [PMID: 27459899]
• De Sousa E Melo F, Wang X, Jansen M et al. Poor-prognosis colon cancer is defined by a molecularly distinct subtype and develops from serrated precursor lesions. Nat Med 2013-05-01 [PMID: 23584090] (IHC-P, Human)
• Kerschner JL, Harris A. Transcriptional networks driving enhancer function in the CFTR gene. Biochem J 2012-09-01 [PMID: 22671145]
• Verzi MP, Shin H, Ho LL et al. Essential and redundant functions of caudal family proteins in activating adult intestinal genes. Mol Cell Biol 2011-05-01 [PMID: 21402776]
• Verzi MP, Hatzis P, Sulahian R et al. TCF4 and CDX2, major transcription factors for intestinal function, converge on the same cis-regulatory regions. Proc Natl Acad Sci U S A 2010-08-01 [PMID: 20696899]

Review for CDX2 Antibody (NB100-2136) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Bryan Tinsley IHC Human 04/21/2014

Summary

• Application: Immunohistochemistry
• Species: Human

Bioinformatics

• Gene Symbol: CDX2
• Entrez:
  • 1045(Human)
• Uniprot:
  • Q99626()