Centrin 1 Antibody - BSA Free

Western Blot: Centrin 1 Antibody [NBP2-14909] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin 1 antibody diluted at a dilution of 1:1000.

Immunoprecipitation: Centrin 1 Antibody [NBP2-14909] - Immunoprecipitation of Centrin-1 protein from 293T whole cell extracts using 5 ug of Centrin-1 antibody [N1C3] . Western blot analysis was performed using Centrin-1 antibody [N1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Immunoprecipitation: Centrin 1 Antibody [NBP2-14909] - A431 cells were fixed in ice-cold MeOH for 5 min.Green: Centrin 1 stained by Centrin 1 antibody [N1C3] diluted at 1:500.Blue: Hoechst 33342 staining.

Western Blot: Centrin 1 Antibody [NBP2-14909] - Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE Centrin-1 antibody NBP2-14909 diluted at 1:1000

• Reactivity: Hu, Bv, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Centrin 1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Centrin 1. The exact sequence is proprietary.
• Predicted Species: Mouse (95%), Rat (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CETN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin Assay dependent
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (84%), Chicken (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Centrin 1 Antibody - BSA Free

• calcium binding protein
• Caltractin isoform 2
• caltractin
• CEN1centrin-1
• centrin, EF-hand protein, 1
• CETN
• EF-hand protein

Background

The protein encoded by this gene plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. This encoded protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CETN1
• Uniprot:
  • Q12798(Human)