Cerberus 1 Antibody - Azide and BSA Free

Western Blot: Cerberus 1 Antibody [NBP2-99622] - Anti-Cerberus 1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HEK293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 28 kDa

Immunoprecipitation: Cerberus 1 Antibody [NBP2-99622] - Cerberus 1 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 1 ul anti-Cerberus 1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Cerberus 1 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 34 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Cerberus 1 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human Cerberus 1 (Accession#: AAH69371.1; Met1-Ala267)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CER1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:25000-1:50000
  • Immunoprecipitation 1-2 uL/mg of lysate
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Cerberus 1 Antibody - Azide and BSA Free

• CER1
• cerberus 1 (Xenopus laevis) homolog (cysteine knot superfamily)
• Cerberus 1
• cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis)
• cerberus
• cerberus-related 1
• Cerberus-related protein
• DAN domain family member 4
• DAND4
• MGC119894
• MGC119895
• MGC96951

Background

Cerberus encodes a cytokine member of the cysteine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CER1