Choline Acetyltransferase/ChAT Antibody

Western Blot: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - Effect of denaturing temperature on Western blot on brain lysates using Rabbit antibody to ChAT.

Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. DAPI counter stained.

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on paraffin sections of rat DRG using Rabbit antibody to ChAT.

Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. DAPI counter stained.

Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord (free floating sections) at 1:1000 dilution using Rabbit antibody to c-terminal region of ChAT (choline O-acetyltransferase): whole serum (NB110-89724).

Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on paraffin sections of rat DRG using Rabbit antibody to ChAT.

• Reactivity: Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Choline Acetyltransferase/ChAT Antibody Summary

• Immunogen: A synthetic peptide from mouse Choline Acetyltransferase/ChAT conjugated to blue carrier protein was used as the antigen.
• Marker: Cholinergic Neuronal Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHAT
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:2000
  • Immunohistochemistry Free-Floating 1:500
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Western Blot 1:500
• Application Notes: This antibody is currently not validating in Immunohistochemistry-Frozen.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for Choline Acetyltransferase/ChAT Antibody

• acetyl CoA:choline O-acetyltransferase
• CHAT
• CHOACTase
• Choline acetylase
• Choline Acetyltransferase
• choline O-acetyltransferase
• CMS1A
• CMS1A2
• EC 2.3.1
• EC 2.3.1.6

Background

Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein. Choline acetylase catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2); also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Choline Acetyltransferase/ChAT Antibody (NB110-89724)(3)

Publications using NB110-89724

• Hatanaka F, Suzuki K, Shojima K et al. Therapeutic strategy for spinal muscular atrophy by combining gene supplementation and genome editing bioRxiv 2023-04-07 (Immunohistochemistry, Mouse)
• Lang SC, Harre U, Purohit P et al. Neurodegeneration Enhances the Development of Arthritis. J. Immunol. 2017-02-10 [PMID: 28188247] (IF/IHC, Mouse)
• Schizas N, Rojas R, Kootala S et al. Hyaluronic acid-based hydrogel enhances neuronal survival in spinal cord slice cultures from postnatal mice. J Biomater Appl 2013-05-14 [PMID: 23674184] (IF/IHC, Mouse)

FAQs for Choline Acetyltransferase/ChAT Antibody (NB110-89724). (Showing 1 - 1 of 1 FAQs).

1. I need Choline Acetyltransferase antibody - neuronal marker for the rat brain and only for immunohistochemistry frozen sections staining. In your catalog I see you have NB110-89724, NBP1-46764 and NB100-64603. Which one of it is suitable for our needs?
   • Regarding your question, all three of those antibodies are suitable for rat frozen sections and you can choose any of the three; however, the immunogen for NB100-64603 is choline acetyltransferase purified from rat brain. Please note that the working dilution we recommend for this antibody is 1:100 to 1:250. Here is our general protocol for IHC-fr for your attention.

Bioinformatics

• Gene Symbol: CHAT
• Entrez:
  • 12647(Mouse)
  • 290567(Rat)
• Uniprot:
  • P32738(Rat)