Chorionic Gonadotropin alpha/beta (hCG) Antibody (HCGab/52)

Immunohistochemistry-Paraffin: Chorionic Gonadotropin alpha/beta (hCG) Antibody (HCGab/52) [NBP2-45334] - Human Placenta stained with Chorionic Gonadotropin alpha/beta (hCG) Monoclonal Antibody (HCGab/52).

• Reactivity: Hu
• Applications: IHC
• Clone: HCGab/52
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Chorionic Gonadotropin alpha/beta (hCG) Antibody (HCGab/52) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11526)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Purified Chorionic Gonadotropin alpha/beta (hCG) protein
• Localization: Cytoplasmic, Secreted
• Marker: Pregnancy & Choriocarcinoma Marker
• Specificity: This monoclonal antibody is a very special because it reacts ONLY with the intact-HCG and not with either free alpha- or free beta-chain of HCG. HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the subunit is identical to that of thyroid stimulating hormone (TSH) follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG monoclonal antibody detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CGB3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Chorionic Gonadotropin alpha/beta (hCG) Antibody (HCGab/52)

• CGB;CGB3;CGB5;CGB7;CGB8;Choriogonadotropin subunit beta 3;Choriogonadotropin subunit beta 7;hCGB;LHB

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CGB3
• Uniprot:
  • P01215()
  • P01215()
  • P01233(Human)