Claudin-5 Antibody (JM11-22)

Immunohistochemistry: Claudin-5 Antibody (JM11-22) [NBP2-66783] - IF staining of D15 kidney organoid (N2 line, ML protocol) for CLDN5 as a marker of early podocyte differentiation derived from the single-cell data. Additional canonical podocyte markers include NPHS1 (blue), WT1 (red) and DAPI (grey) staining. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-13382-0) licensed under a CC-BY license.

Western Blot: Claudin-5 Antibody (JM11-22) [NBP2-66783] - Analysis of Claudin 5 on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: Claudin-5 Antibody (JM11-22) [NBP2-66783] - Analysis of paraffin-embedded human kidney tissue using anti-Claudin 5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Claudin-5 Antibody (JM11-22) [NBP2-66783] - Analysis of paraffin-embedded human lung tissue using anti-Claudin 5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Claudin-5 Antibody (JM11-22) [NBP2-66783] - Analysis of paraffin-embedded human tonsil tissue using anti-Claudin 5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Claudin-5 Antibody (JM11-22) [NBP2-66783] - Analysis of paraffin-embedded human uterus tissue using anti-Claudin 5 antibody. Counter stained with hematoxylin.

Product Discontinued

Claudin-5 Antibody (JM11-22) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Claudin-5 aa 191-214 / 218. (SwissProt: O00501 Human)
• Localization: Cell junction. Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CLDN5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Claudin-5 Antibody (JM11-22)

• AWAL
• AWALTransmembrane protein deleted in VCFS
• BEC1
• claudin 5
• Claudin5
• Claudin-5
• CLDN5
• CPETRL1
• TMDVCF
• TMVCF1
• TMVCFTMDVCF
• transmembrane protein deleted in velocardiofacial syndrome

Background

The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specific regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-5 is expressed in the endothelial junctions of the rat liver and in junctions of acinar cells of the pancreas. Human claudin-5 is abundantly expressed in adult lung, heart and skeletal muscle and is deleted in patients with velocardiofacial syndrome, which is characterized by cleft palate, facial dysmorphology and conotruncal heart defects.The human claudin-5 gene maps to chromosome 22q11.21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claudin-5 Antibody (NBP2-66783)(1)

Publication using NBP2-66783

• Subramanian A, Sidhom EH, Emani M et al. Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation Nat Commun 2019-11-29 [PMID: 31784515] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: CLDN5