CLEC4D/CLECSF8 Antibody (WEN42) - Azide Free

Flow Cytometry: CLEC4D/CLECSF8 Antibody (WEN42) [NBP3-11193] - Detection of CLEC4D/CLECSF8 in transfected BW5147 cells. Red and blue line represent IgG1 Isotype control and CLEC4D/CLECSF8 stained cells respectively.

• Reactivity: Rt
• Applications: WB, Flow, Func, IHC, IP
• Clone: WEN42
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide Free
• Concentration: 0.1 mg/ml

CLEC4D/CLECSF8 Antibody (WEN42) - Azide Free Summary

• Immunogen: BWZ.36 cell expressing chimeric CLEC4D/CLECSF8
• Specificity: WEN42 does not bind to cells transfected with human MCL or with other APLEC receptors.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC4D
• Purity: Protein G purified
• Endotoxin Note: <24 EU/mg

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Functional
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
• Application Notes: For immunohistochemistry, Flow Cytometry, immunoprecipitation and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for CLEC4D/CLECSF8 Antibody (WEN42) - Azide Free

• CD368
• CLEC4D
• CLEC6
• CLEC-6
• CLECSF8
• CLECSF8Mpcl
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 8
• C-type lectin domain family 4 member D
• C-type lectin domain family 4, member D
• C-type lectin receptor
• C-type lectin superfamily member 8
• C-type lectin-like receptor 6
• macrophage C-type lectin
• MCL
• MGC40078
• Mpcl

Background

The monoclonal antibody WEN42 recognizes rat macrophage C-type lectin (MCL). C-type lectin receptors (CLRs) have recently been identified as a fourth family of pattern-recognition receptors for pathogen associated molecular patterns, following TLRs, Nod-like receptors and RIG-I-like receptors. MCL is a membrane surface receptor encoded by the Antigen Presenting Lectin-like gene Complex (APLEC).The complex consists of seven related C-type lectin receptor genes, namely, Dendritic Cell Activating Receptor (DCAR), Dendritic Cell Inhibitory Receptor 1, 2, 3 and 24 (DCIR), Macrophage C-type lectin (MCL) and Macrophage inducible C-type lectin (Mincle). MCL is a type II transmembrane protein with a single extracellular C-terminal C-type lectin-like domain. C-type lectins are functionally diverse. Human MCL is expressed on macrophages (synovial, peritoneal, and blood monocyte-derived), Langerhans cells and on neutrophils, monocytes and immature and mature DCs. In rats, expression of MCL has been detected on macrophages, neutrophils, DCs, B cells, and T cells. Their presence on the surface of immune cells and their potential for recognizing polysaccharide structures suggests a central role as pattern-recognition receptors (PRR) in the innate immune system. Despite the growing amount of data describing expression and function of the APLEC receptors, very little has been reported about MCL in general and the rat MCL in particular. Studies investigating the role of MCL has demonstrated that MCL is capable of inducing phagocytosis, cytokine production and oxidative burst, suggesting an activating role for this protein in the immune response. WEN42 does not bind to cells transfected with human MCL or with other APLEC receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLEC4D