Cofilin 2 Antibody

Western Blot: Cofilin 2 Antibody [NBP1-33570] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 2 antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody [NBP1-33570] - Paraformaldehyde-fixed HeLa, using Cofilin 2 (muscle) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.

Western Blot: Cofilin 2 Antibody [NBP1-33570] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000

Western Blot: Cofilin 2 Antibody [NBP1-33570] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 2 antibody (NBP1-33570) diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, RM
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Cofilin 2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cofilin 2. The exact sequence is proprietary.
• Localization: Nucleus matrix, Cytoplasm, cytoskeleton
• Predicted Species: Porcine (100%), Bovine (100%), Rhesus Macaque (100%), Chicken (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CFL2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (89%), Xenopus laevis (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Cofilin 2 Antibody

• cofilin 2 (muscle)
• Cofilin, muscle isoform
• cofilin-2
• NEM7

Background

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. (Gillett et al., 1996 [PubMed 8800436]). Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN; MIM 609114), all of which regulate actin-filament dynamics (Bamburg et al., 1999 [PubMed 10461190]; Maciver and Hussey, 2002). The CFL2 gene encodes a skeletal muscle-specific isoform (Vartiainen et al., 2002 [PubMed 11809832]) localized to the thin filaments, where it exerts its effect on actin, in part through interactions with tropomyosins (Ono and Ono, 2002 [PubMed 11901171]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CFL2