Collagen I Antibody - BSA Free

Rat colon tissue stained with Collagen I antibody (red) and Hoechst (blue). IHC-P image submitted by a verified customer review.

Myofibroblast differentiation requires CD271/NGF activation. Representative alphaSMA (red) and COLLAGEN 1 (red) immunostaining with DAPI (blue) in non-treated (control), TGFbeta-treated cells with or without gamma-secretase inhibitor. Scale bars: 50 um Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-09992-3) licensed under a CC-BY license.

Lane 1: Human Collagen Type 1. Lane 2: None. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1000 overnight at 4C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms.

Representative Western blot image for Collagen I, Collagen 2 and GAPDH. N/group = 8. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.12367) licensed under a CC-BY license.

Macro- and micro-architecture of human adipose tissue lobule. Representative immunostaining of the human AT lobule with Collagen I (COL1), CD34, and DAPI. The position of the septa is underlined, scale bar: 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-09992-3) licensed under a CC-BY license.

Primary human cardiac fibroblast cells were stained with anti-Collagen I antibody. Cells were cultured for 3 days in DMEM with 10% fetal calf serum. ICC/IF image submitted by a verified customer review.

Cell lysate from human trabecular meshwork. Antibody at 1:1.000. WB image submitted by a verified customer review.

Patient-derived CAF and NF CDMs are architecturally and functionally distinct. (e) Collagen I (red) and fibronectin (blue) staining of patient #1 and #3 NF and CAF CDM. Scale bar, 20 μm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms12237) licensed under a CC-BY license.

Staining of human HaCaT-ras A5-RT3 (SCC) frozen tumor sections. Image provided by Dr. Wa'el Al Rawashdeh of RWTH Aachen University.

Livers were harvested at 9 wpi, formalin-fixed, and then cryosectioned and stained for markers of inflammation and liver fibrosis. Maximum intensity projections of 12-17 um thick liver sections with immunofluorescently labeled nuclei (DAPI white) alpha-smooth muscle actin NBP2-34760APC (yellow), CD45 (red) and Collagen I NB600-408 (top) or Collagen III/COL3A1 NB600-594 (bottom) (blue) in the liver of uninfected or 9 wpi WT mice. Scale bars represent 50 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32973293/) licensed under a CC-BY license.

Analysis in porcine burn wound lysate using anti-Collagen I alpha 1 antibody. WB image submitted by a verified customer review.

Detection of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane). Protein staining shown below each blot depicts equal protein loading. An equal amount of the whole cell protein (100 ug) was separated by SDS-PAGE and electroblotted to nitro-cellulose membranes. Proteins were detected by incubating the membrane with anti-Collagen I antibody at a concentration of 0.2-2 ug/10 mL in TBS (100 mM Tris-HCl, 0.15 M NaCl, pH 7.4) with 5% non-fat milk. Detection occurred by incubation with a HRP conjugated secondary antibody at 1 ug/10 mL. Proteins were detected by a chemiluminescent method using the PIERCE ECL kit (Amersham Biosciences).

Balb/3T3 mouse embryonic fibroblast cell line stained with Collagen I antibody. IHC-P image submitted by a verified customer review.

Mouse pancreas tissue stained with Collagen I antibody. IHC-P image submitted by a verified customer review.

Human lung tissue section stained with Collagen I antibody at 1:400.

FFPE right lobe of the liver tissue section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:1250 for 4C for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type I is intra and extracellular. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.

Analysis of FFPE human lung. Primary antibody: Collagen I 1:400. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Analysis of HRP conjugate of NB600-408. FFPE Human Skin at pH 6. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: Collagen Type I is secreted in the extracellular matrix.

Analysis of HRP conjugate of NB600-408. FFPE Human Skin tissue at pH9. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).

Imaging of rat calvarial defect bone. IHC-P image submitted by a verified customer review.

Immunohistological staining of EG treated rat kidneys for Collagen. Magnification X40, Scale bar 100 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0185009), licensed under a CC-BY license.

Analysis of Collagen I Antibody on alcohol-fixed rat lung tissue. Primary antibody dilution 1:200. Image from verified customer review.

Immunohistochemistry of Collagen I antibody. Tissue: human lung. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: Collagen 1 1:400 Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Immunohistochemistry of Rabbit Anti-collagen type I antibody. Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:1250 for 4C for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type I is intra and extracellular. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.

Immunohistochemistry-Paraffin: Rabbit Polyclonal Collagen I Antibody [NB600-408] - Collagen I was stained in human FFPE tissue. Primary antibody dilution: 1/1000 in blocking buffer. HIER antigen retrieval at pH 9 for 20min. AF750 conjugated version of the antibody was used (Catalog # NB600-408AF750). Image from a verified customer review.

Effects of fasudil treatment on tumor stroma in KPC mice.A) Pancreatic tumor tissues from vehicle, gemcitabine, or combination of gemcitabine plus fasudil treated KPC mice were harvested and stained for various stromal markers. Representative images are shown of H&E staining, alpha -SMA, Desmin, CD31, Collagen I, and Movat's pentachrome staining.

Western blot analysis. Comparison of collagen 1 (A and B) and collagen 2 (D and E) among the anterior horn, the body and the posterior horn: (A and D) young model; (B and E) adult model. Comparison of collagen 1 (C) and collagen 2 (F) content between the young and the adult specimens. Values with different superscripts differ for P < 0.01 (A,B). (G) Representative Western blot image for collagen 1, collagen 2 and GAPDH. N/group = 8.

EPS15 is targeted via a SPOP/SPOPL binding consensus motif.(A) Cartoon of human EPS15 domain-organization and the amino-acid sequence. Indicated by color code are the SPOP/SPOPL binding site (red) and the lysine residue (yellow), which is ubiquitinated in a CRL3SPOPL–dependent manner in vivo. In addition, the amino-terminal Ca2+-binding EF-hand motifs (EH), the coiled-coil domain involved in dimerization and the two carboxy-terminal ubiquitin-interacting motifs (UIMs) involved in ubiquitin-binding are indicated. (B) EPS15 ubiquitin-profiling. Peptides containing EPS15 modification sites were quantified with LC-MS/MS after enrichment of the K-epsilon -GG motif from whole cell digests of HeLa cells treated with siSPOPL or siControl. Normalized precursor mass intensity profiles for EPS15 sites corresponding to K793, K801 and K693 are shown (raw data in Figure 4—figure supplement 1B). Quantification of the beta -Actin K113 and the polyubiquitin K11 linkage peptide control for comparable enrichment. Data are mean ± SD, N = 3. **p≤0.01. (C) Purified SPOPL was incubated as indicated with GST-tagged wild-type EPS15 or GST-EPS15 mutants, where the predicted SPOPL binding motifs have been mutated individually (GST-EPS15S605-S607A and EPS15S744-S746A, respectively), pulled down with glutathione sepharose (IP [GST]) and bound proteins were analyzed by Coomassie blue staining (upper panel) and immunoblotting (lower panels). Note that SPOPL readily binds to GST-EPS15 and GST-EPS15S605-S607A, but this interaction is strongly reduced with the GST-EPS15S744-S746A mutant. (D) HeLa cells stably expressing GFP-tagged wild-type EPS15, the EPS15S744-S746A or the EPS15K793R mutants from a doxycycline-inducible promoter were transfected as indicated (+) with control siRNA or siRNA depleting SPOPL. The levels of EPS15-GFP, EGFR and for control tubulin (TUB) were analyzed by immunoblotting with specific antibodies. Experiments were quantified in Fiji and the EPS15 levels plotted as fold-increase compared to controls. Data are mean ± SEM, N = 4. *p≤0.05. Note that SPOPL depletion does not further increase the levels of both EPS15 mutants. (E) Total cell extracts were prepared from HeLa cells expressing either GFP-tagged wild-type, the EPS15S744-S746A mutant or the EPS15K793R mutant in the presence (+) or absence (-) of HA-tagged SPOPL overexpression. The levels of EPS15-GFP, SPOPL-HA and control GAPDH were analyzed by immunoblotting. Note that overexpression of SPOPL-HA is able to induce degradation of wild-type but not the EPS15S744-S746A-GFP or the EPS15K793R-GFP mutant.DOI://dx.doi.org/10.7554/eLife.13841.009EPS15 is targeted via a SPOP/SPOPL binding consensus motif.(A) Alignments of the carboxy-terminal domains of EPS15 proteins from various species. Conserved SPOPL-binding motifs and putative ubiquitination sites are highlighted by yellow boxes. (B) Peptides containing EPS15 modification sites were quantified with LC-MS/MS after enrichment of the K-epsilon -GG motif from whole cell HeLa digests treated with siSPOPL and siControl. Raw intensities for each of the triplicate LC-MS/MS runs are shown with each of the siControl conditions scaled to 100% intensity. Normalized precursor mass intensity profiles for EPS15 sites corresponding to K793, K801 and K693 are shown, with only K793 showing significant downregulation in the depletion condition. Quantification of a peptide corresponding to beta -Actin K113 and the poly-ubiquitin K11 linkage peptide is also shown to demonstrate that enrichment variations did not influence the quantification of the EPS15 sites. Additionally, the total ion chromatographic intensities for each run are plotted to provide insight into the consistency of each of the separate experiments performed on different days. Data are mean ± SD, N = 3. (C) HeLa cell lines stably expressing wild-type EPS15-GFP, the EPS15S744-746A-GFP mutant or the EPS15K793R-GFP mutant from the inducible doxycycline-promoter were treated with doxycycline for 3 days, and analyzed by live cell imaging. Displayed are maximal projections of Z-stack acquisitions, fully covering cell height. Scale bar = 10 μm.DOI://dx.doi.org/10.7554/eLife.13841.010 Image collected and cropped by CiteAb from the following open publication (//elifesciences.org/articles/13841), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Local release of anti-IL-6 from IMB suppresses LN fibrosis. The skin allografts were harvested at 7 days post-transplantation. (A-i) Lymphatic vessel expansion (Lyve-1+) & HEV elongation (MECA79+) were similar between all groups. (A-ii,iii) Dense staining of collagen I & PDPN was seen in DLNs harvested from control & GelMA group compared to those from GelMA/anti-IL-6 group. (representative images from 4 different mice per group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31024011), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Phf2 protects the liver from high fat & high sucrose diet-induced fibrogenesis during obesity. GFP or Phf2 were overexpressed through AAV strategy specifically in the liver of 7-week-old male C57Bl/6 J mice. Mice were then fed with either a chow diet (NCD) or with a high fat & high sucrose diet (HFHSD) for 16 weeks. Mice were studied in the fed state. a Representative western blot analysis showing the contribution of Phf2 overexpression to the regulation of the PI3K/Akt signaling & pro-fibrogenic pathways in liver extracts (n = 15 per group). b (Left) Oral glucose tolerance test & insulin levels during the OGTT test. (right) Insulin tolerance test (n = 15 per group). c Liver sections stained with hematoxylin & eosin (H&E), trichrome masson & sirius red are shown. Scale bars = 100 μm (n = 10 per group). d Percentage of fibrotic area & percentage of apoptotic hepatocytes (n = 15 per group). e Relative oxidized GSSG content & measurement of Gpx activity (n = 10 per group). f Levels of carbonylated proteins (n = 10 mice per group). All error bars represent mean ± SEM. Statistical analyses were made using unpaired t-test (b) or Anova, followed by Bonferonni’s test (d, e). *P < 0.01 HFHSD/GFP compared to HFHSD/Phf2, $P < 0.01 HFHSD/GFP compared to NCD/GFP, £P < 0.01 HFHSD/Phf2 compared to HFHSD/GFP Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04361-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Collagen I Antibody [NB600-408] - alpha -SMA, LOX, & COL1A1 immunostaining in human thyroid cancers. Representative thyroid tumors serial sections from two different patients stained by IHC for alpha -SMA, COL1A1, & LOX protein expression & localization. In the top panel, the tumor edge/invasive front is specifically shown (scale bar 500 µm), while lower panel has higher magnification (scale bar 200 µm). * clusters of tumor invading cells detaching from the principal tumor mass (T). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906302), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Increased co-localization of collagen type I with ER stress marker, KDEL in post-mortem human glaucomatous TM tissues. Post-mortem human TM tissues from age-matched normal (n = 3) & glaucoma (n = 3) were stained with Col I (green) & KDEL (red) & co-localization was examined in anterior segment tissues. Representative images are shown at lower (A) scale bar is 100 microns) & higher (B) scale bar is 25 microns) magnification. Both Col I & KDEL was increased in human glaucomatous TM tissues. In addition, increased co-localization of Col I with KDEL was observed in human glaucomatous TM tissues. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14938-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - Expression of osteoblastic differentiation markers in Human Gingival Mesenchymal Stem Cells. Adherent HGMSCs were cultured for 21 days in control medium or in differentiation medium (Diff) supplemented or not with 1 μM resveratrol (RV). At the end, cell homogenates were processed for western blotting analysis of the expression of the osteoblastic transcription factor RUNX-2 & of the osteogenic differentiation markers COL1A1, OPN & OCN. Densitometry of the specific bands (average ± S.D.) of three independent experiments is shown in the histograms Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31426798), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Dex induced ECM synthesis is associated with ER stress in TM cells. Primary human TM cells (n = 3 strains) were treated with Veh or Dex (100 nM) for 3 days & stained for KDEL & fibronectin (A) or Col I (B). A prominent co-localization of fibronectin or Col I with KDEL was observed in Dex-treated TM cells indicating induction of ER stress & excessive intracellular ECM overload in primary TM cells. (C) GTM3 cells were treated with Veh or Dex for 3 days & total lysates were subjected to immunoprecipitation using KDEL antibody & immunoblotted for ECM proteins, GRP78 & GRP94. IgG antibody was used as a negative control. Total cellular lysates were used as input. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14938-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Dex increases Col I in the TM: Dex increases Col I & alpha -smooth muscle actin in the TM of Dex-treated mice (n = 3) compared with Veh-treated mice (n = 3). White box indicates TM. CB = ciliary body, I = Iris, C = cornea. Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-14938-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Collagen I Antibody [NB600-408] - alpha -SMA, LOX, & COL1A1 immunostaining in human thyroid cancers. Representative thyroid tumors serial sections from two different patients stained by IHC for alpha -SMA, COL1A1, & LOX protein expression & localization. In the top panel, the tumor edge/invasive front is specifically shown (scale bar 500 µm), while lower panel has higher magnification (scale bar 200 µm). * clusters of tumor invading cells detaching from the principal tumor mass (T). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31906302), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - Spautin-1 prevents the expression of osteoblastic differentiation markers in Human Gingival Mesenchymal Stem cells. HGMSCs were cultured for 21 days in control medium or in differentiation medium supplemented with 1 μM resveratrol (Diff + RV) in the absence or in the presence of spautin-1 (Sp1). At the end, cell homogenates were processed for western blotting analysis of the expression of the osteoblastic transcription factor RUNX-2 & of the osteogenic differentiation markers COL1A1, OPN & OCN. Densitometry of the specific bands (average ± S.D.) of three independent experiments is shown in the histograms Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31426798), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Collagen I Antibody [NB600-408] - Effects of fasudil treatment on tumor stroma in KPC mice.A) Pancreatic tumor tissues from vehicle, gemcitabine, or combination of gemcitabine plus fasudil treated KPC mice were harvested & stained for various stromal markers. Representative images are shown of H&E staining, alpha -SMA, Desmin, CD31, Collagen I, & Movat's pentachrome staining. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28841710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Immunofluorescence staining of osteoblastic differentiation markers in Human Gingival Mesenchymal Stem Cells. HGMSCs were plated on sterile coverslips, let adhere & cultured for 21 days in control medium or in differentiation medium (Diff) supplemented or not with 1 μM resveratrol (RV). At the end, the coverslips were fixed & processed for immunofluorescence staining of the osteogenic differentiation markers. Fluorescence staining was quantified with the ImageJ software Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31426798), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - Collagen secretion defects in fibroblasts from patient 1a. (a) Control or patient fibroblasts incubated for 30 min to promote procollagen secretion were immunolabeled to detect COL1A1 (red) & in green, either Sec31A (to mark COPII‐coated ER exit sites) or GM130 (to mark the Golgi apparatus). (b) Extracellular collagen matrix was labeled to detect COL1A1. (c) Quantification of the mean Corrected Total Cell Fluorescence (CTCF) for COL1A1. Points show mean intensity from three independent experiments. Bars show standard deviation. (d) Immunoblot for COL1A1 in media (M) or lysates (L) of control or patient fibroblasts incubated in the presence of absence of ascorbate. (e) Unbiased proteomics was used to measure extracellular matrix collagens secreted from control or patient fibroblasts. Results from three independent experiments are shown. A ratio <1 indicates reduced collagen secretion from patient cells Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31568717), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - The effects of PA on TGF-beta 1-reduced PPAR gamma expression in HSC-T6 cells. (A,B) The effect of PA-induced PPAR gamma expression in rat hepatic stellate cells (HSCs). Cells were treated with PA (2 μM) for 0, 6, 12, & 24 h or PA (0.5, 1, & 2 μM) for 24 h, & PPAR gamma expression was analyzed by Western blotting. The effect of PA on TGF-beta 1-reduced PPAR gamma expression in rat HSCs; (C) cells were pretreated with various concentrations of PA (0.5, 1, & 2 μM) for 1 h, & then stimulated with TGF-beta 1 (5 ng/mL) for 24 h. Total protein extracted from cells was subjected to Western blotting to determine PPAR gamma expression; (D,E) cells were treated with TGF-beta 1 (5 ng/mL) for 24 h in the presence of PA and/or GW9662 (10 μM). Total protein extracted from cells was subjected to Western blotting to determine the expression of PPAR gamma & alpha -SMA & collagen I alpha 1. Protein bands were imaged using densitometry & analyzed using ImageJ software. The relative expression levels of target proteins were normalized using beta -actin as an internal control. The results are presented as the means ± SD of three independent experiments. # Significantly different from the control (p < 0.01). * Significantly different from the TGF-beta 1-treated group (p < 0.01). + Significantly different from the PA-treated group (p < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31795488), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - The effects of PA on TGF-beta 1-induced alpha -SMA & collagen I alpha 1 expression via suppression of the Akt & MAPK signal pathways in HSC-T6 cells. (A,B) The inhibitory effects of PA on TGF-beta 1-induced alpha -SMA & collagen I alpha 1 expression via suppression of Akt & MAPK in rat hepatic stellate cells (HSCs); (A) cells were treated with TGF-beta 1 (5 ng/mL) for 24 h in the presence of LY294002 (LY; 10 μM), PD98059 (PD; 20 μM), SB203580 (SB; 20 μM), or SP600125 (SP; 20 μM); (B) cells were treated with TGF-beta 1 (5 ng/mL) for 24 h in the presence of PA & LY294002 (LY; 10 μM), PD98059 (PD; 20 μM), SB203580 (SB; 20 μM), or SP600125 (SP; 20 μM). Total protein extracted from cells was subjected to Western blotting to determine alpha -SMA & collagen I alpha 1 expression. Protein bands were imaged using densitometry & analyzed using ImageJ software. The relative expression levels of target proteins were normalized using beta -actin as an internal control. The results are presented as the means ± SD of three independent experiments. # Significantly different from the control (p < 0.01). * Significantly different from the TGF-beta 1-treated group (p < 0.01). + Significantly different from the PA-treated group (p < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31795488), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Cells expressing IL-1⍺ & IL-1R are observed in the fibrotic liver environment. (a) Cytokines in tissue lysates from mice at 9 wpi were measured by ELISA in liver. Data are presented as fold change relative to the mean of uninfected levels. N = 11–12 mice per group, pooled from three independent experiments. (b) IL-1 alpha levels in the sera at 9 wpi measured by ELISA. N = 9–14 mice per group, pooled from four independent experiments. (c) Immunofluorescence labeling of nuclei (DAPI white), IL-1⍺ (green), CD45 (red), & collagen1⍺1 (blue) in the liver of UI or 9 wpi WT mice. Number of cells staining positive for CD45 and/or IL-1⍺, average 2–3 fields of view where immune infiltrate was present from 3 mice per condition are quantified on the right. Error bars are standard deviation. (d) Immunofluorescent labeling of nuclei (DAPI white), ⍺-smooth muscle actin (green), IL-1R (red), & collagen1⍺ (blue) in the liver of uninfected or 9 wpi WT. Inset, arrow head represents ⍺-smooth muscle actin/IL-1R co-staining cells (arrow heads). Number of cells staining positive for IL-1R and/or ⍺-SMA, average of 4–8 fields of view from 3 mice are quantified on the right. Error bars are standard deviation. (c,d) represent maximum intensity projections of 9–13 μm thick z-stacks. Scale bar represents 50 μm. Error bars are standard error of the mean except where noted otherwise. *P < 0.05; **P < 0.01; ***P < 0.001 by unpaired Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32973293), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: Collagen I Antibody [NB600-408] - Characterization of decellularized heart matrix for DNA & extracellular matrix content with DNA quantification, histological staining, & multiphoton imaging. (A) DNA content of the right ventricle (RV, triangles), septum (ST, circles), & left ventricle (LV, squares) for cadaveric hearts compared to decellularized Langendorff (n = 4) or 4-Flow cannulated hearts (n = 4). (B) Masson’s trichrome staining of representative Langendorff (n = 4) & 4-Flow (n = 4) hearts for preserved collagen (blue) & clearance of nuclear remnants (absence of positively stained purple nuclei). (C) Multiphoton microscopy visualization of collagen (pseudocolored - cyan) & elastin (pseudocolored - yellow) fibrillar strcutures & organization in 3D (n = 4). (D) Immunostaining of histology sections important extracellular matrix proteins: collagen I, collagen IV, & laminin (n = 4). (E) The list of preserved extracellular matrix proteins in decellularized 4-Flow hearts as detected by mass spectrometry (n = 4). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-25883-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - PARP1 regulates VSMCs osteogenic transition, not Na/P co-transporter. a, b Quantitative RT-PCR (a) & western blot (b) analysis of PIT1 & PIT2 expression in VSMCs, which were infected with Scrambled (Scr shRNA) or PARP1 shRNA, & treated with high Pi. c–e Rat VSMCs were infected with adenovirus carrying Scr shRNA, PARP1 shRNA, Null or PARP1 gene for 48 h & then incubated with osteogenic media for 14 days. PARP1 inhibitors 3AB (10 mM) & PJ34 (10 μM) were added every day. Western blot analysis & related quantification of osteogenic-related marker genes (OPN, ColIA1, & OC) & smooth muscle marker genes (SMA, SM22 & SMMHC) expression in VSMCs with PARP1 knockdown (c), PARP1 inhibitors 3AB & PJ34 (d), & PARP1 overexpression (e). (n = 5 for each group). Statistical significance was assessed using one-way ANOVA for multiple comparison & two-tailed t-tests for two groups & is presented as follows: NS: no significance, **P < 0.01 & ##P < 0.01. All values are means ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30867423), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - CM derived from OS cells stimulates the BM‐MSCs trans‐differentiation into CAF‐like cells. (A) FACS analysis of NG‐2 & CD31 expression in BM‐MSCs treated for 48 h with CM OS cells. (B) BM‐MSCs were stimulated for 48 h with CM from OS cells & alpha ‐SMA & Col I‐ alpha 1 expression was assessed by immunoblot analysis. Results are representative of four biological replicates. (C) Collagen contraction assay of BM‐MSCs treated for 24 h with St Med or HOS CM. Data are expressed as percentages of the relative area of collagen disc following contraction in comparison with an empty well (mean ± SD, n = 3 biological replicates performed in duplicate). * P < 0.05 vs. St Med. (D) Migration assay of HOS cells stimulated for 48 h with CM derived from BM‐MSCs previously activated or not activated by tumour cells (CM BM‐MSCs St/HOS). Cells were allowed to migrate toward complete medium (FBS 10%). Untreated cells (St Med) were used as control. Results are the mean ± SEM of three biological replicates. *** P < 0.001 vs. St Med. (E) Invasion & transmigration (F) assays of HOS cells treated as in (D) (mean ± SD, n = 3 independent experiments). ** P < 0.005 vs. St Med; *** P < 0.001 vs. St Med. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29517849), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Human PRP infusion improves regeneration of the damaged endometrium & down-regulates expression of fibrosis-related factors. (A) Design of Experiment 1. In AS mice, PRP was injected only into the right horn at 7 days after inducing injury to the bilateral uterine horns. At 14 days, all mice were sacrificed & the uterine horns were prepared for analysis. (B) Hematoxylin & eosin staining of the endometrial tissues to evaluate morphologic structures in the PRP-treated horn of AS mice. (C) Masson’s trichrome staining to evaluate collagen deposition (blue) in the PRP-treated horns of AS mice. (D) Immunofluorescence staining of collagen type 1A (COL1A1) in the PRP-treated horn of AS mice. Green & red indicate COL1A1 & nuclei, respectively. (E) RT-qPCR & (F) real-time RT-qPCR analyses of expression of fibrosis-related factors in the PRP-treated horn of AS mice. Sham, n = 4; AS or PRP-treated AS, n = 5. *p < 0.01; Scale bar: 100 & 200 μm. AS, Asherman’s syndrome; PCR, polymerase chain reaction; PRP, platelet-rich plasma; RT-qPCR, reverse transcription-quantitative PCR. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32116803), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - The effects of PA on TGF-beta 1-induced alpha -SMA & collagen I alpha 1 expression via suppression of the Akt & MAPK signal pathways in HSC-T6 cells. (A,B) The inhibitory effects of PA on TGF-beta 1-induced alpha -SMA & collagen I alpha 1 expression via suppression of Akt & MAPK in rat hepatic stellate cells (HSCs); (A) cells were treated with TGF-beta 1 (5 ng/mL) for 24 h in the presence of LY294002 (LY; 10 μM), PD98059 (PD; 20 μM), SB203580 (SB; 20 μM), or SP600125 (SP; 20 μM); (B) cells were treated with TGF-beta 1 (5 ng/mL) for 24 h in the presence of PA & LY294002 (LY; 10 μM), PD98059 (PD; 20 μM), SB203580 (SB; 20 μM), or SP600125 (SP; 20 μM). Total protein extracted from cells was subjected to Western blotting to determine alpha -SMA & collagen I alpha 1 expression. Protein bands were imaged using densitometry & analyzed using ImageJ software. The relative expression levels of target proteins were normalized using beta -actin as an internal control. The results are presented as the means ± SD of three independent experiments. # Significantly different from the control (p < 0.01). * Significantly different from the TGF-beta 1-treated group (p < 0.01). + Significantly different from the PA-treated group (p < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31795488), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Fibroblast-derived CDM induces sustained growth inhibition of cancer cells.(a) Collagen I & fibronectin staining of CDM generated by NFs (TIFFs). Scale bar, 20 μm. (b) Proliferation of MDA-MB-231 & HeLa cells plated on TIFF CDM or on plastic in full medium for the indicated times. n(HeLa)=10, n(MDA-MB-231)=8. (c) Proliferation of MDA-MB-231 & HeLa cells after detachment from TIFF matrices (6 days on matrix before detachment) & replating on plastic in full medium for the indicated times n(HeLa)=7, n(MDA-MB-231)=10. (d) Representative images of MDA-MB-231 cell morphology on CDM & plastic. Shown are maximum intensity projections of confocal images. Scale bar, 10 μm. (e) Schematic representation of the experimental set-up. Red arrows indicate the time points of sample collection for Illumina gene expression analysis. (f) Common gene expression changes in MDA-MB-231 & HeLa cells on CDM & 5 days after CDM detachment (CDM to plastic (pl.)). The numbers of commonly regulated genes (up- or downregulated) in both cell lines are indicated in the table. Upregulated genes are marked with red & downregulated genes with blue. All data are mean±s.e.m. Unpaired t-test was used for statistical analyses. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27488962), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Spautin-1 prevents the autophagy-associated osteoblastic differentiation of Human Gingival Mesenchymal Stem cells. a HGMSCs were plated on sterile coverslips, let adhere & cultured for 21 days in control medium or in differentiation medium supplemented with 1 μM resveratrol (Diff + RV) in the absence or the presence of spautin-1 (Sp1). The coverslips were then fixed & processed for immunofluorescence staining of the autophagy marker LC3 & of the osteoblastic differentiation marker OCN. As an index of autophagy in the cells, LC3 puncta were quantified (as per the guidelines 36). b HGMSCs were plated & treated as in a & the coverslips processed for immunofluorescence staining of the osteoblastic differentiation markers OCN & COL1A1. Integrated fluorescence intensity quantified with the ImageJ software is shown in the histograms. c HGMSCs cells were plated on plastic & treated as in a & at the end the cultures were processed for Alizarin Red S staining of extracellular calcium deposits. The stained area was quantified using the ImageJ software Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31426798), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Collagen secretion defects in fibroblasts from patient 1a. (a) Control or patient fibroblasts incubated for 30 min to promote procollagen secretion were immunolabeled to detect COL1A1 (red) & in green, either Sec31A (to mark COPII‐coated ER exit sites) or GM130 (to mark the Golgi apparatus). (b) Extracellular collagen matrix was labeled to detect COL1A1. (c) Quantification of the mean Corrected Total Cell Fluorescence (CTCF) for COL1A1. Points show mean intensity from three independent experiments. Bars show standard deviation. (d) Immunoblot for COL1A1 in media (M) or lysates (L) of control or patient fibroblasts incubated in the presence of absence of ascorbate. (e) Unbiased proteomics was used to measure extracellular matrix collagens secreted from control or patient fibroblasts. Results from three independent experiments are shown. A ratio <1 indicates reduced collagen secretion from patient cells Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31568717), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Collagen I Antibody [NB600-408] - Cells expressing IL-1⍺ & IL-1R are observed in the fibrotic liver environment. (a) Cytokines in tissue lysates from mice at 9 wpi were measured by ELISA in liver. Data are presented as fold change relative to the mean of uninfected levels. N = 11–12 mice per group, pooled from three independent experiments. (b) IL-1 alpha levels in the sera at 9 wpi measured by ELISA. N = 9–14 mice per group, pooled from four independent experiments. (c) Immunofluorescence labeling of nuclei (DAPI white), IL-1⍺ (green), CD45 (red), & collagen1⍺1 (blue) in the liver of UI or 9 wpi WT mice. Number of cells staining positive for CD45 and/or IL-1⍺, average 2–3 fields of view where immune infiltrate was present from 3 mice per condition are quantified on the right. Error bars are standard deviation. (d) Immunofluorescent labeling of nuclei (DAPI white), ⍺-smooth muscle actin (green), IL-1R (red), & collagen1⍺ (blue) in the liver of uninfected or 9 wpi WT. Inset, arrow head represents ⍺-smooth muscle actin/IL-1R co-staining cells (arrow heads). Number of cells staining positive for IL-1R and/or ⍺-SMA, average of 4–8 fields of view from 3 mice are quantified on the right. Error bars are standard deviation. (c,d) represent maximum intensity projections of 9–13 μm thick z-stacks. Scale bar represents 50 μm. Error bars are standard error of the mean except where noted otherwise. *P < 0.05; **P < 0.01; ***P < 0.001 by unpaired Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32973293), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Collagen I Antibody [NB600-408] - The effects of PA on TGF-beta 1-induced alpha - SMA & collagen I alpha 1 expression in HSC-T6 cells. (A,B) The inhibitory effect of PA on TGF-beta 1-induced alpha -SMA & collagen type I mRNA & protein expression in rat hepatic stellate cells. Cells were pretreated with 0.5, 1, & 2 μM PA for 1 h, & then stimulated with TGF-beta 1 (5 ng/mL) for 24 h. Total RNA extracted from cells was analyzed by the real-time polymerase chain reaction to determine alpha -SMA & ColIa1 mRNA expression; (C) the total protein extracted from cells was subjected to Western blotting to determine alpha -SMA & collagen I alpha 1 expression. Protein bands were imaged using densitometry & analyzed using ImageJ software. The relative expression levels of target proteins were normalized using beta -actin as an internal control. The results are expressed as the means ± SD of three independent experiments. # Significantly different from the control (p < 0.01). * Significantly different from the TGF-beta 1-treated group (p < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31795488), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Frozen: Collagen I Antibody [NB600-408] - Cryopreserved murine brain tissue stained with Collagen I Antibody [Alexa Fluor® 647] (Catalog # NB600-408AF647) (red) and Hoechst (blue). Image from a verified customer review.

• Reactivity: Hu, Mu, Rt, Po, Bv, Rb
• Applications: WB, ELISA, ICC/IF, IHC, IP, MA, PAGE, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Collagen I Antibody - BSA Free Summary

• Description: This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
  
  Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Immunogen: Collagen I from human and bovine placenta (Uniprot: P02452)
• Localization: Extracellular matrix
• Specificity: Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL1A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:50000
  • Fluorophore-linked immunosorbent assay 1:100
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100
  • Knockdown Validated
  • Microarray
  • SDS-Page
  • Western Blot 1:1000-1:10000
• Application Notes: This product has been tested by dot blot and IHC and are useful for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, Immunofluorescence, FLOW, and western blotting for highly sensitive qualitative analysis.
  
  Use in SDS-Page reported in scientific literature (PMID: 30914477).

Reactivity Notes

This antibody reacts with human, bovine, and most mammalian Type I collagens with negligible cross-reactivity with Type II, III, IV, V or VI collagens
Use in Mouse reported in scientific literature (PMID: 33758176).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Collagen I Antibody - BSA Free

• Alpha-1 type I collagen
• alpha1(I) procollagen
• CAFYD
• COL1A1
• Collagen 1
• collagen alpha 1 chain type I
• collagen alpha-1(I) chain preproprotein
• collagen alpha-1(I) chain
• Collagen I
• collagen of skin, tendon and bone, alpha-1 chain
• collagen, type I, alpha 1
• EDSARTH1
• EDSC
• OI1
• OI2
• OI3
• OI4
• pro-alpha-1 collagen type 1
• type I proalpha 1
• Type I Procollagen Alpha 1 Chain

Background

Collagen I is one of the most abundant extracellular matrix (ECM) proteins in mammals and is an important structural component in connective tissues, bones, teeth, skin, heart, and lungs (1, 2). Collagen I is commonly used for biomedical purposes including providing an ECM microenvironment for cell attachment, proliferation, and mesenchymal stem cell growth and differentiation to the osteogenic lineage (1, 3, 4). Furthermore, type I Collagen hydrogels are a promising scaffold for engineering tissues and tumors (4). Collagen type I can be extracted from numerous sources including mammals, most commonly bovine and porcine, fish, amphibians, and birds (1, 4). Structurally, Collagen I is a heterotrimer comprised of two alpha-1 chains and one alpha-2 chain that together forms a triple helix (1, 3, 5). Each alpha chain is primarily formed by glycine, proline, and hydroxyproline repeats with the alpha-1 chains having a theoretical molecular weight of 139 kDa and 129 kDa for the alpha-2 chain (3). The dimeric form of Collagen I has a theoretical molecular weight of ~270 kDa while the trimeric form is >400 kDa (3, 5).

A variety of disease pathologies have been associated with mutations in or disruptions in collagen type I. Osteogenesis imperfecta, also known as brittle bone disease, is caused by autosomal dominant mutations in Collagen type I encoding genes and characterized by fragile bones and skeletal deformities (6). Ehlers-Danlos syndromes (EDS) is another disorder that affects soft connective tissue and its various clinical subtypes are associated with different mutations in Collagen I (7).

Alternative names for Collagen I includes alpha-1 type I collagen, alpha1(I) procollagen, CAFYD, COL1A1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, pro-alpha-1 collagen type 1, type I proalpha 1, and type I procollagen alpha 1 chain.

References

1. Chowdhury, S. R., Mh Busra, M. F., Lokanathan, Y., Ng, M. H., Law, J. X., Cletus, U. C., & Binti Haji Idrus, R. (2018). Collagen Type I: A Versatile Biomaterial. Advances in experimental medicine and biology. https://doi.org/10.1007/978-981-13-0947-2_21

2. Kisling, A., Lust, R. M., & Katwa, L. C. (2019). What is the role of peptide fragments of collagen I and IV in health and disease?. Life sciences. https://doi.org/10.1016/j.lfs.2019.04.042

3. Kwon, D., Kang, G. S., Han, D. K., Park, K., Kim, J. H., & Lee, S. H. (2014). Establishment and characterization of human engineered cells stably expressing large extracellular matrix proteins. Archives of pharmacal research. https://doi.org/10.1007/s12272-013-0294-0

4. Antoine, E. E., Vlachos, P. P., & Rylander, M. N. (2014). Review of collagen I hydrogels for bioengineered tissue microenvironments: characterization of mechanics, structure, and transport. Tissue engineering. Part B, Reviews. https://doi.org/10.1089/ten.TEB.2014.0086

5. Leon-Lopez, A., Morales-Penaloza, A., Martinez-Juarez, V. M., Vargas-Torres, A., Zeugolis, D. I., & Aguirre-alvarez, G. (2019). Hydrolyzed Collagen-Sources and Applications. Molecules (Basel, Switzerland). https://doi.org/10.3390/molecules24224031

6. Marini, J. C., Forlino, A., Bachinger, H. P., Bishop, N. J., Byers, P. H., Paepe, A., Fassier, F., Fratzl-Zelman, N., Kozloff, K. M., Krakow, D., Montpetit, K., & Semler, O. (2017). Osteogenesis imperfecta. Nature reviews. Disease primers. https://doi.org/10.1038/nrdp.2017.52

7. Callewaert, B., Malfait, F., Loeys, B., & De Paepe, A. (2008). Ehlers-Danlos syndromes and Marfan syndrome. Best practice & research. Clinical rheumatology, 22(1), 165-189. https://doi.org/10.1016/j.berh.2007.12.005

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen I Antibody (NB600-408)(288)

Publications using NB600-408

• Jack H. Wellmerling, Sara R. Dresler, Jeffrey A. Meridew, Kyoung M. Choi, Daniel J. Tschumperlin, Qi Tan RNA ‐sequencing reveals differential fibroblast responses to bleomycin and pneumonectomy Physiological Reports 2024-07-11 [PMID: 38991987]
• Biswajoy Ghosh, Jyotirmoy Chatterjee, Ranjan Rashmi Paul, Sebastian Acuña, Pooja Lahiri, Mousumi Pal, Pabitra Mitra, Krishna Agarwal Molecular histopathology of matrix proteins through autofluorescence super-resolution microscopy Scientific Reports 2024-05-08 [PMID: 38719976]
• Salome Guillaumin, Mehmet Gurdal, Dimitrios I. Zeugolis, Gustavo Caetano-Anolles Gums as Macromolecular Crowding Agents in Human Skin Fibroblast Cultures Life 2024-03-25 [PMID: 38672707]
• Jan-Niklas May, Jennifer I Moss, Florian Mueller, Susanne K Golombek, Ilaria Biancacci, Larissa Rizzo, Asmaa Said Elshafei, Felix Gremse, Robert Pola, Michal Pechar, Tomáš Etrych, Svea Becker, Christian Trautwein, Roman D Bülow, Peter Boor, Ruth Knuechel, Saskia von Stillfried, Gert Storm, Sanyogitta Puri, Simon T Barry, Volkmar Schulz, Fabian Kiessling, Marianne B Ashford, Twan Lammers Histopathological biomarkers for predicting the tumour accumulation of nanomedicines. Nature biomedical engineering 2024-04-08 [PMID: 38589466]
• Yiwei Wang, Xingchen Geng, Zhihua Guo, Dandan Chu, Ruixing Liu, Boyuan Cheng, Haohao Cui, Chengcheng Li, Jingguo Li, Zhanrong Li M2 macrophages promote subconjunctival fibrosis through YAP/TAZ signalling Annals of Medicine 2024-02-09 [PMID: 38335557]
• Salli Talvi, Johanna Jokinen, Kalle Sipilä, Pekka Rappu, Fu-Ping Zhang, Matti Poutanen, Pia Rantakari, Jyrki Heino Embigin deficiency leads to delayed embryonic lung development and high neonatal mortality in mice. iScience 2024-02-16 [PMID: 38318368]
• Rajadurai A, Tsao H Identification of collagen-suppressive agents in keloidal fibroblasts using a high-content, phenotype-based drug screen JID Innovations 2023-11-01 [PMID: 38303762] (WB)
• Camarena, V;Williams, MM;Morales, AA;Zafeer, MF;Kilic, OV;Kamiar, A;Abad, C;Rasmussen, MA;Briski, LM;Peart, L;Bademci, G;Barbouth, DS;Smithson, S;Wang, G;Shehadeh, LA;Walz, K;Tekin, M; ADAMTSL2 mutations determine the phenotypic severity in Geleophysic Dysplasia JCI insight 2024-02-01 [PMID: 38300707]
• Yoon-Young Kang, Dong-Young Kim, Sang-Yong Lee, Hee-Joong Kim, Taehawn Kim, Jeong A Cho, Taewon Lee, Eun Young Choi Innate Immune Training Initiates Efferocytosis to Protect against Lung Injury. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024-01-26 [PMID: 38279580]
• Laura Jahnke, Virginie Perrenoud, Souska Zandi, Yuebing Li, Federica Maria Conedera, Volker Enzmann, Girish Kumar Srivastava Modulation of Extracellular Matrix Composition and Chronic Inflammation with Pirfenidone Promotes Scar Reduction in Retinal Wound Repair Cells 2024-01-16 [PMID: 38247855]
• Junichi Saito, Jui M. Dave, Freddy Duarte Lau, Daniel M. Greif Presenilin-1 in smooth muscle cells facilitates hypermuscularization in elastin aortopathy iScience 2023-12-13 [PMID: 38226162]
• Amy Lewis, David T Humphreys, Belen Pan-Castillo, Giulio Berti, Carla Felice, Hannah Gordon, Radha Gadhok, Anke Nijhuis, Shameer Mehta S, Liliane Eleid, Sidra Iqbal, Alessandro Armuzzi, Annamaria Minicozzi, Eleni Giannoulatou, Joanne ChinAleong, Roger Feakins, Virag Sagi-Kiss, Dora Barisic, Margarita-Ioanna Koufaki, Jacob G Bundy, James O Lindsay, Andrew Silver Epigenetic and Metabolic Reprogramming of Fibroblasts in Crohn’s Disease Strictures Reveals Histone Deacetylases as Therapeutic Targets Journal of Crohn's & Colitis 2024-06-01 [PMID: 38069679]
• Yang Q, Huo E, Cai Y et al. Myeloid PFKFB3-mediated glycolysis promotes kidney fibrosis Frontiers in immunology 2023-11-16 [PMID: 38035106] (WB, Mouse)
• Zhang K, Zhang MX, Meng XX et al. Targeting GPR65 alleviates hepatic inflammation and fibrosis by suppressing the JNK and NF-?B pathways Military Medical Research 2023-11-25 [PMID: 38001521] (WB, IHC, Mouse, Human)
• Gäberlein K, Schröder SK, Nanda I et al. Genetic Characterization of Rat Hepatic Stellate Cell Line PAV-1 Cells 2023-06-11 [PMID: 37371073] (WB, Rat)
• Zhao T, Chu Z, Chu C et al. Macrophages induce gingival destruction via Piezo1-mediated MMPs-degrading collagens in periodontitis Frontiers in Immunology 2023-05-16 [PMID: 37261355] (ICC/IF, Human, Mouse)
  
  Details:
  Dilution: 1:100
• Drakos S, Shankar T, Visker J et al. Adipocyte Enhancer Binding Protein 1 (AEBP1) Inhibition as a Potential Anti-Fibrotic Therapy in Heart Failure. Research Square 2023-10-17 (WB, Mouse)
• Zhu X, Wang Y, Soaita I et al. Acetate controls endothelial-to-mesenchymal transition Cell metabolism 2023-06-08 [PMID: 37327791] (WB, Human)
• Drakos S, Shankar T, Visker J et al. Adipocyte Enhancer Binding Protein 1 (AEBP1) Inhibition as a Potential Anti-Fibrotic Therapy in Heart Failure. Research Square 2023-10-17 (IHC, Mouse)
• Liu S, Li B, Liu N et al. Multi-omics analysis of human tendon adhesion reveals ACKR1 migrates macrophages involve in endogenous regeneration Research Square 2023-10-30 (IHC, Human)
• Scaria SM, Frumm SM, Vikram EP et al. Epimorphic regeneration in the mammalian tympanic membrane NPJ Regenerative medicine 2023-10-18 [PMID: 37852984] (IHC-WhMt, Mouse)
• Lee KW, Chung K, Nam DH et al. Decellularized allogeneic cartilage paste with human costal cartilage and crosslinked hyaluronic acid-carboxymethyl cellulose carrier augments microfracture for improved articular cartilage repair Acta biomaterialia 2023-10-07 [PMID: 37813156] (IHC, Rabbit)
• Ballester-Servera C, Alonso J, Cañes L et al. Lysyl oxidase-dependent extracellular matrix crosslinking modulates calcification in atherosclerosis and aortic valve disease Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023-09-18 [PMID: 37729730]
• Hernández-Bule ML, Toledano-Macías E, Pérez-González LA et al. Anti-Fibrotic Effects of RF Electric Currents International journal of molecular sciences 2023-07-01 [PMID: 37446165] (ICC/IF)
• Silva CS, Pinto RD, Pires RA et al. The Influence of Feeder Cell-Derived Extracellular Matrix Density on Thymic Epithelial Cell Culture ACS biomaterials science & engineering 2023-04-19 [PMID: 37074315]
• Beamish EL, Johnson J, Shih B et al. Delay in loop ileostomy reversal surgery does not impact upon post-operative clinical outcomes. Complications are associated with an increased loss of microflora in the defunctioned intestine Gut microbes 2023-04-14 [PMID: 37055940] (WB, Human)
• Gao AY, Diaz Espinosa AM, Nguyen BBN et al. Dopamine Receptor D1 Is Exempt from Transforming Growth Factor ?-Mediated Antifibrotic G Protein-Coupled Receptor Landscape Tampering in Lung Fibroblasts The Journal of pharmacology and experimental therapeutics 2023-09-01 [PMID: 37024146]
• Liu B, Jie X, Deng J et al. Bupi Yishen formula may prevent kidney fibrosis by modulating fatty acid metabolism in renal tubules Phytomedicine : international journal of phytotherapy and phytopharmacology 2023-03-16 [PMID: 37001297] (IHC-P, Rat)
• Li S, Livingston MJ, Ma Z et al. Tubular cell senescence promotes maladaptive kidney repair and chronic kidney disease after cisplatin nephrotoxicity JCI insight 2023-03-14 [PMID: 36917180] (IHC-P, WB, Mouse)
• Paul K, Darzi S, Del Borgo M et al. Vaginal delivery of tissue engineered endometrial mesenchymal stem/stromal cells in an aloe vera-alginate hydrogel alleviates maternal simulated birth injury Applied Materials Today 2021-03-01 (ICC/IF, Human)
• Volkova E, Procell L, Kong L et al. Vascular stiffening in aging females with a hypertension induced HIF2A gain of function mutation Bioengineering & Translational Medicine 2022-10-03 [PMID: 36925716] (IF/IHC, Mouse)
• DeRoo E, Zhou T, Yang H et al. A vein wall cell atlas of murine venous thrombosis determined by single-cell RNA sequencing Communications biology 2023-01-31 [PMID: 36721040] (Immunohistochemistry, Mouse)
• Zhang Y, Jeong H, Mori K et al. Vascular endothelial growth factor from retinal pigment epithelium is essential in choriocapillaris and axial length maintenance PNAS Nexus 2022-08-24 [PMID: 36714840] (IF/IHC, Mouse)
• Lee SY, Kim S, Choi I et al. Inhibition of 11 beta -hydroxysteroid dehydrogenase 1 relieves fibrosis through depolarizing of hepatic stellate cell in NASH Cell death & disease 2022-11-29 [PMID: 36446766] (WB, Human)
  
  Details:
  Dilutions: 1:500
• Scaria S Understanding the Mechanisms of Epimorphic Regeneration in the Mammalian Tympanic Membrane Thesis 2022-01-01 (Mouse)
• Takács R, Vágó J, Póliska S et al. The temporal transcriptomic signature of cartilage formation Nucleic acids research 2023-05-08 [PMID: 36987858] (IHC-P, Chicken)
  
  Details:
  1:500 IHC-P dilution
• Kwon T, Moon D, Kim J Et Al. Application of 630-nm and 850-nm Light-emitting Diodes and Microcurrent to Accelerate Collagen and Elastin Deposition in Porcine Skin Medical Lasers 2021-06-30 (IHC-P)
• Alaghemand H, Esmaeili B, Firouz P. A comparative study of the preventive effect of chlorhexidine o. 12% and nano zinc oxide particles on the distraction of collagen scaffolding of the hybrid layer by two immunohistochemistry and microleakage tests. Dentistry and Medical research. 2014-09-21 (IHC-P, Human)
  
  Details:
  Collagen I alpha 1 antibody used for IHC-P on 4um sections of dentin extracted from human subject's molars and premolar teeth with no caries and visible anatomic defects (Figure 2).
• Arias-Garcia M, Rickman R, Sero J The cell-cell adhesion protein JAM3 determines nuclear deformability by regulating microtubule organization biorxiv.org 2019-01-01 (ICC/IF, Human)
• Shao Q, Feng Y, Wang W et al. Drug carrier for sustained release of withaferin A for pancreatic cancer treatment J Mater Sci
• Tibaldi E, Brocca A, Sticca A et al. Fam20C-mediated phosphorylation of osteopontin is critical for its secretion but dispensable for its action as a cytokine in the activation of hepatic stellate cells in liver fibrogenesis FASEB j. Nov 29 2019 (WB, Rat)
• Melchor SJ, Hatter JA, Castillo EALT, Saunders CM Cachexia and fibrosis are costs of chronic IL-1R-mediated disease tolerance in T. gondii infection bioRxiv
• Shao Q, Feng Y, Wang W et al. Drug carrier for sustained release of withaferin A for pancreatic cancer treatment J Mater Sci 2019 Oct 23
• Rodriguez-Rivera V. Novel Biofabrication Technologies to Recapitulate In Vivo Geometries in Collagen Hydrogels Thesis. 2014-12-15 (WB)
• Alves AL, Carvalho AC, Machado I et al. Cell-Laden Marine Gelatin Methacryloyl Hydrogels Enriched with Ascorbic Acid for Corneal Stroma Regeneration Bioengineering (Basel, Switzerland) 2023-01-04 [PMID: 36671634] (ICC/IF, Human)
  
  Details:
  Dilution used in ICC 1:200
• Cook J. Toward a Mechanistic Understanding of Hepatic Insulin Action and Resistance. Thesis. 2014-01-01 (WB, Mouse)
• Yuri G, Sanhueza S, Paredes A et al. Deleterious liver-adipose crosstalk in obesity: Hydroethanolic extract of Lampaya medicinalis Phil. (Verbenaceae) counteracts fatty acid-induced fibrotic marker expression in human hepatocytes Molecular and cellular endocrinology 2023-03-15 [PMID: 36736687]
• Latif A, Fisher LE, Dundas AA et al. Microparticles Decorated with Cell-Instructive Surface Chemistries Actively Promote Wound Healing Advanced materials (Deerfield Beach, Fla.) 2022-11-28 [PMID: 36440539] (IHC-P, Human)
• Sun X, Zhou L, Han Y et al. Scutellarin Attenuates Doxorubicin-Induced Cardiotoxicity by Inhibiting Myocardial Fibrosis, Apoptosis and Autophagy in Rats Chemistry & biodiversity 2022-11-23 [PMID: 36419360]
• Taniguchi Y, Ouhara K, Kitagawa M et al. Periapical lesion following Cnm-positive Streptococcus mutans pulp infection worsens cerebral hemorrhage onset in an SHRSP rat model Clinical and experimental immunology 2022-11-11 [PMID: 36368020] (WB, Human)
• Mi YB, Liu XH, Wang WS et al. ER-phagy Is Involved in the Degradation of Collagen I by IL-1B in Human Amnion in Parturition Journal of immunology (Baltimore, Md. : 1950) 2022-10-26 [PMID: 36288908] (WB, Human)
• Lewis A, SAnchez S, Berti G et al. Small-molecule Wnt inhibitors are a potential novel therapy for intestinal fibrosis in Crohns disease Clinical science (London, England : 1979) 2022-10-14 [PMID: 36156078] (ICC/IF, Human)
• Gong H, Chen L, He Y et al. Pleural thickening induced by Glaesserella parasuis infection was linked to increased collagen and elastin Frontiers in cellular and infection microbiology 2022-08-11 [PMID: 36034702] (IHC-P, Porcine)
• Zhou YH, Guo Y, Zhu JY et al. Spheroid co-culture of BMSCs with osteocytes yields ring-shaped bone-like tissue that enhances alveolar bone regeneration Scientific reports 2022-08-27 [PMID: 36030312] (IHC-P, Mouse)
  
  Details:
  IHC-P 1:100
• Caporarello N, Lee J, Pham TX et al. Dysfunctional ERG signaling drives pulmonary vascular aging and persistent fibrosis Nature communications 2022-07-25 [PMID: 35879310] (WB, Human)
• Hadjisavva R, Anastasiou O, Ioannou PS et al. Adherens junctions stimulate and spatially guide integrin activation and extracellular matrix deposition Cell reports 2022-07-19 [PMID: 35858563] (ICC, Human)
• Ron C Vinestock, Neta Felsenthal, Eran Assaraf, Eldad Katz, Sarah Rubin, Lia Heinemann-Yerushalmi, Sharon Krief, Nili Dezorella, Smadar Levin-Zaidman, Michael Tsoory, Stavros Thomopoulos, Elazar Zelzer Neonatal Enthesis Healing Involves Noninflammatory Acellular Scar Formation through Extracellular Matrix Secretion by Resident Cells. The American journal of pathology 2022-08-16 [PMID: 35659946]
• Li X, Yong T, Wei Z et al. Reversing insufficient photothermal therapy-induced tumor relapse and metastasis by regulating cancer-associated fibroblasts Nature communications 2022-05-19 [PMID: 35589680] (IF/IHC, Mouse, Human)
• Xue W, Yu SY, Kuss M et al. 3D bioprinted white adipose model forin vitrostudy of cancer-associated cachexia induced adipose tissue remodeling Biofabrication 2022-05-26 [PMID: 35504266]
• Park HH, Kim YM, Anh Hong LT et al. Dual-functional hydrogel system for spinal cord regeneration with sustained release of arylsulfatase B alleviates fibrotic microenvironment and promotes axonal regeneration Biomaterials 2022-05-01 [PMID: 35461098] (IF/IHC, Rat)
• Pothiawala A, Sahbazoglu BE, Ang BK et al. GDF5 + chondroprogenitors derived from human pluripotent stem cells preferentially form permanent chondrocytes Development (Cambridge, England) 2022-04-22 [PMID: 35451016]
• Aidos L, Modina SC, Millar VRH et al. Meniscus Matrix Structural and Biomechanical Evaluation: Age-Dependent Properties in a Swine Model Bioengineering (Basel, Switzerland) 2022-03-15 [PMID: 35324808] (IF/IHC, WB, Porcine)
• Trott AJ, Greenwell BJ, Karhadkar TR et al. Lack of food intake during shift work alters the heart transcriptome and leads to cardiac tissue fibrosis and inflammation in rats BMC biology 2022-03-03 [PMID: 35236346] (IF/IHC, Rat)
• Li C, Yang M, Qu Z et al. Effect of electroacupuncture on the degradation of collagen in pelvic floor supporting tissue of stress urinary incontinence rats International urogynecology journal 2022-02-28 [PMID: 35226143] (WB, Rat)
• Rusu-Nastase E, Lupan A, Marinescu C et al. MiR-29a Increase in Aging May Function as a Compensatory Mechanism Against Cardiac Fibrosis Through SERPINH1 Downregulation Frontiers in Cardiovascular Medicine 2022-01-18 [PMID: 35118144] (ICC/IF, Mouse)
• Srinivasan MP, Bhopale KK, Caracheo AA et al. Exposure to binge ethanol and fatty acid ethyl esters exacerbates chronic ethanol-induced pancreatic injury in hepatic alcohol dehydrogenase deficient deer mice American journal of physiology. Gastrointestinal and liver physiology 2022-01-05 [PMID: 34984929] (WB, Mouse)
• Wang R, Kairen C, Li L et al. Overexpression of NDUFV1 alleviates renal damage by improving mitochondrial function in unilateral ureteral obstruction model mice Cell biology international 2021-12-22 [PMID: 34936716]
• Keller CR, Hu Y, Ruud KF Et al. Human Breast Extracellular Matrix Microstructures and Protein Hydrogel 3D Cultures of Mammary Epithelial Cells Cancers 2021-11-22 [PMID: 34831010] (ICC/IF, Human)
• Kasetti RB, Maddineni P, Kodati B Et al. Astragaloside IV Attenuates Ocular Hypertension in a Mouse Model of TGF beta 2 Induced Primary Open Angle Glaucoma International journal of molecular sciences 2021-11-19 [PMID: 34830390] (ICC/IF, Human)
• de Sousa BM, Correia CR, Ferreira JAF Et al. Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants NPJ Regenerative medicine 2021-11-23 [PMID: 34815414] (WB, Mouse)
• Lee J, Ung A, Kim H Et al. Engineering liver microtissues to study the fusion of HepG2 with mesenchymal stem cells and invasive potential of fused cells Biofabrication 2021-11-30 [PMID: 34740205]
• Miura Y, Lam M, Bourke JE, Kanazawa S Bimodal fibrosis in a novel mouse model of bleomycin-induced usual interstitial pneumonia Life science alliance 2022-01-01 [PMID: 34728556] (IHC-P, Mouse)
• Huang LT, Chou HC, Chen CM. Inhibition of FABP4 attenuates hyperoxia-induced lung injury and fibrosis via inhibiting TGF- beta signaling in neonatal rats Journal of cellular physiology 2021-10-28 [PMID: 34708870] (Rat)
• Maddineni P, Kasetti RB, Kodati B et al. Sodium 4-Phenylbutyrate Reduces Ocular Hypertension by Degrading Extracellular Matrix Deposition via Activation of MMP9 International journal of molecular sciences 2021-09-18 [PMID: 34576258] (ICC/IF, Mouse)
• Chen M, Wen X, Gao Y et al. IRF-4 deficiency reduces inflammation and kidney fibrosis after folic acid-induced acute kidney injury International immunopharmacology 2021-09-20 [PMID: 34555644] (WB, Mouse)
• Zhao S, Li W, Yu W et al. Exosomal miR-21 from tubular cells contributes to renal fibrosis by activating fibroblasts via targeting PTEN in obstructed kidneys Theranostics 2021-08-02 [PMID: 34522205] (WB, Mouse)
• Zhu L, Xu F, Kang X et al. The antioxidant N-acetylcysteine promotes immune response and inhibits epithelial-mesenchymal transition to alleviate pulmonary fibrosis in chronic obstructive pulmonary disease by suppressing the VWF/p38 MAPK axis Molecular medicine (Cambridge, Mass.) 2021-09-03 [PMID: 34479474] (WB, Rat)
• Maity P, Singh K, Krug L Et al. Persistent JunB activation in fibroblasts disrupts stem cell niche interactions enforcing skin aging Cell reports 2021-08-31 [PMID: 34469740] (ICC/IF, Mouse)
• Zhang S, Wei C, Wang Q Et Al. M2-Polarized Macrophages Mediate Wound Healing by Regulating Connective Tissue Growth Factor via AKT, ERK 1/2, and STAT 3 Signaling Pathways Research Square 2021-06-22 [PMID: 34398425] (IF/IHC)
• Montaldo C, Terri M, Riccioni V Et Al. Fibrogenic signals persist in DAA-treated HCV patients after sustained virological response Journal of hepatology 2021-07-13 [PMID: 34271004]
• Giacalone D, Huang L, Tan S Exploiting Fluorescent Proteins to Understand Mycobacterium tuberculosis Biology Methods in molecular biology (Clifton, N.J.) 2021-07-08 [PMID: 34235663]
• Xie T, Kulur V, Liu N et al. Mesenchymal growth hormone receptor deficiency leads to failure of alveolar progenitor cell function and severe pulmonary fibrosis Science advances 2021-06-01 [PMID: 34108218] (IF/IHC, Mouse)
• Ishii H, Kaneko S, Yanai K et al. MicroRNA Expression Profiling in Diabetic Kidney Disease Translational research : the journal of laboratory and clinical medicine 2021-06-06 [PMID: 34102327] (IF/IHC, Mouse)
• Song Y, Kim S, Heo J et al. Identification of hepatic fibrosis inhibitors through morphometry analysis of a hepatic multicellular spheroids model Scientific reports 2021-05-25 [PMID: 34035369] (WB, Human)
• Ouyang L, Su X, Li W et al. ALKBH1-demethylated DNA N6-methyladenine modification triggers vascular calcification via osteogenic reprogramming in chronic kidney disease The Journal of clinical investigation 2021-05-18 [PMID: 34003800] (WB, Human)
• Stevenson NL, Bergen DJM, Lu Y et al. Giantin is required for intracellular N-terminal processing of type I procollagen The Journal of cell biology 2021-06-07 [PMID: 33944912]
• Ji S, Gumina D, McPeak K et al. Human placental villous stromal extracellular matrix regulates fetoplacental angiogenesis in severe fetal growth restriction Clinical science (London, England : 1979) 2021-04-27 [PMID: 33904582]
• Suzuki K, Komura M, Obana K et al. A new method of primary engineering of esophagus using orthotopic in-body tissue architecture Journal of Pediatric Surgery 2021-03-01 [PMID: 33845983]
• Li W, Qi N, Wang S et al. miR-455-5p regulates atrial fibrillation by targeting suppressor of cytokines signaling 3 Journal of physiology and biochemistry 2021-04-01 [PMID: 33792885]
• Maier JI, Rogg M, HelmstAdter M et al. EPB41L5 controls podocyte extracellular matrix assembly by adhesome-dependent force transmission Cell reports 2021-03-23 [PMID: 33761352] (ICC/IF, Human)
• Altera A, Tosi GM, Regoli M et al. The extracellular matrix complexity of idiopathic epiretinal membranes and the bilaminar arrangement of the associated internal limiting membrane in the posterior retina Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2021-03-24 [PMID: 33760980]
• Li P, Liu Y, Qin X et al. SIRT1 attenuates renal fibrosis by repressing HIF-2 alpha Cell death discovery 2021-03-23 [PMID: 33758176] (WB, Mouse)
• Cho HJ, Kim HJ, Lee K et al. Bioengineered Multicellular Liver Microtissues for Modeling Advanced Hepatic Fibrosis Driven Through Non-Alcoholic Fatty Liver Disease Small (Weinheim an der Bergstrasse, Germany) 2021-03-09 [PMID: 33690979]
• Morimoto C, Takedachi M, Kawasaki K et al. Hypoxia stimulates collagen hydroxylation in gingival fibroblasts and periodontal ligament cells Journal of periodontology 2021-03-04 [PMID: 33660864]
• Huang L, Zhang Y, Yang J et al. EGR 1 plays a protective role in AMPK inhibitor compound C induced apoptosis through ROS induced ERK activation in skin cancer cellsAnti fibrotic effects and the mechanism of action of miR 29c in silicosis Molecular medicine reports 2021-04-01 [PMID: 33649785]
• Estermann M, Spiaggia G, Septiadi D et al. Design of Perfused PTFE Vessel-Like Constructs for In Vitro Applications Macromolecular bioscience 2021-02-24 [PMID: 33624920] (IHC-P, Human)
• Rustenhoven J, Drieu A, Mamuladze T, et al. Functional characterization of the dural sinuses as a neuroimmune interface Cell 2021-01-18 [PMID: 33508229]
• Wang H, Xu Y, Wang P, et al. Cell-mediated injectable blend hydrogel-BCP ceramic scaffold for in situ condylar osteochondral repair Acta biomaterialia 2021-01-13 [PMID: 33453407] (IHC-P, Rabbit)
• Kim S, Hong HS Substance-P prevents the cholestatic liver injury by regulating inflammatory responses Peptides 2021-01-10 [PMID: 33440226] (WB, Rat)
• Jian Z, Zhuang T, Qinyu T et al. 3D bioprinting of a biomimetic meniscal scaffold for application in tissue engineering Bioactive materials 2021-06-01 [PMID: 33313450] (ICC/IF, Rabbit)
• O Shea TM, Wollenberg AL, Kim JH et al. Foreign body responses in mouse central nervous system mimic natural wound responses and alter biomaterial functions Nature communications 2020-12-04 [PMID: 33277474] (IHC-Fr, Mouse)
• Mia MM, Cibi DM, Abdul Ghani SAB et al. YAP/TAZ deficiency reprograms macrophage phenotype and improves infarct healing and cardiac function after myocardial infarction PLoS biology 2020-12-01 [PMID: 33264286] (IHC-Fr, Mouse)
  
  Details:
  Transgenic mouse hearts underwent immunohistochemical analysis.
• Diaz-Espinosa, A M, Link, P A Et al. Dopamine D1 receptor stimulates cathepsin K-dependent degradation and resorption of collagen I in lung fibroblasts. J Cell Sci Dec 11 2020 [PMID: 33172983] (Flow, Rat)
  
  Details:
  Citation using the Alexa Fluor 488 format of this antibody.
• Pawan Noel, Shaimaa Hussein, Serina Ng, Corina E. Antal, Wei Lin, Emily Rodela, Priscilla Delgado, Sanna Naveed, Michael Downes, Yin Lin, Ronald M. Evans, Daniel D. Von Hoff, Haiyong Han Triptolide targets super-enhancer networks in pancreatic cancer cells and cancer-associated fibroblasts Oncogenesis 2020-11-09 [PMID: 33168807]
• Kasetti RB, Patel PD, Maddineni P et al. ATF4 leads to glaucoma by promoting protein synthesis and ER client protein load Life (Basel) 2020-11-02 [PMID: 33154371] (IHC-P)
• Chai, Q, Lu, Z Et al. Lung gene expression signatures suggest pathogenic links and molecular markers for pulmonary tuberculosis, adenocarcinoma and sarcoidosis. Commun Biol 2020-10-23 [PMID: 33097805] (WB, IF/IHC, Human)
• An M, Kim H, Moon JM et al. Enzyme-Treated Zizania latifolia Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation Antioxidants (Basel, Switzerland) 2020-09-24 [PMID: 32987843] (IF/IHC, WB, Mouse)
• Melchor, S J, Hatter, J A Et al. T. gondii infection induces IL-1R dependent chronic cachexia and perivascular fibrosis in the liver and skeletal muscle. Sci Rep 2020-09-24 [PMID: 32973293] (WB, Human)
• Dawson LA, Schanes PP, Marrero L et al. Proximal digit tip amputation initiates simultaneous blastema and transient fibrosis formation and results in partial regeneration Wound Repair Regen 2020-08-19 [PMID: 32815252]
• Mietus CJ, Lackner TJ, Karvelis PS et al. Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia J Clin Med 2020-08-08 [PMID: 32784470] (ICC/IF, Human, Bovine)
• Esquibel CR, Wendt KD, Lee HC et al. Second Harmonic Generation Imaging of Collagen in Chronically Implantable Electrodes in Brain Tissue Front Neurosci 2020-07-07 [PMID: 32733179]
• Yang H, An J, Choi I et al. Region-specific astrogliosis: differential vessel formation contributes to different patterns of astrogliosis in the cortex and striatum Mol Brain 2020-07-22 [PMID: 32698847] (IF/IHC, Mouse)
• Caporarello N, Meridew JA, Aravamudhan A et al. Vascular dysfunction in aged mice contributes to persistent lung fibrosis Aging Cell 2020-07-21 [PMID: 32691484] (IF/IHC, Human)
• Wang J, Li G, Hu L et al. Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells Stem Cells Int 2020-06-27 [PMID: 32676119] (IHC-P, Porcine)
• Li X, Zhao J, Kasinath V et al. Lymph node fibroblastic reticular cells deposit fibrosis-associated collagen following organ transplantation J. Clin. Invest. 2020-06-29 [PMID: 32597832] (IHC-Fr, Human)
  
  Details:
  Citation using the Non-Recombinant Monoclonal format of this antibody.
• Gounani Z, Sen Karaman D, Venu AP et al. Coculture of P. aeruginosa and S. aureus on cell derived matrix - An in vitro model of biofilms in infected wounds J. Microbiol. Methods 2020-06-25 [PMID: 32593628] (ICC/IF, Bacteria)
• Lee BJ, Hegewisch Solloa E, Shannon MJ, Mace EM Generation of cell-derived matrices that support human NK cell migration and differentiation J. Leukoc. Biol. 2020-05-11 [PMID: 32392635] (ICC/IF, Human)
• Tran-Nguyen TK, Xue J, Feghali-Bostwick C et al. CD70 Activation Decreases Pulmonary Fibroblast Production of Extracellular Matrix Proteins Am. J. Respir. Cell Mol. Biol. 2020-04-22 [PMID: 32320626]
• Kang Y, Roh MR, Rajadurai S et al. Hypoxia and HIF-1 alpha Regulate Collagen Production in Keloids J. Invest. Dermatol. 2020-04-18 [PMID: 32315657]
• Kim J, Park M, Paek J et al. Intrauterine Infusion of Human Platelet-Rich Plasma Improves Endometrial Regeneration and Pregnancy Outcomes in a Murine Model of Asherman's Syndrome Front. Physiol. 2020-02-14 [PMID: 32116803] (IHC-Fr, Mouse)
• Martina Lerche, Alberto Elosegui-Artola, Jenny Z. Kechagia, Camilo Guzmán, Maria Georgiadou, Ion Andreu, Donald Gullberg, Pere Roca-Cusachs, Emilia Peuhu, Johanna Ivaska Integrin Binding Dynamics Modulate Ligand-Specific Mechanosensing in Mammary Gland Fibroblasts iScience 2020-02-13 [PMID: 32106057]
• Purdy MP, Ducharme M, Haak AJ et al. YAP/TAZ are Activated by Mechanical and Hormonal Stimuli in Myometrium and Exhibit Increased Baseline Activation in Uterine Fibroids Reprod Sci 2020-02-13 [PMID: 32056132]
• Khamar P, Nishtala K, Shetty R et al. Early biological responses in ocular tissue after SMILE and LASIK surgery Exp. Eye Res. 2020-01-27 [PMID: 32001250] (IHC-P, Human)
• Canes L, Marti-Pamies I, Ballester-Servera C et al. Neuron-derived orphan receptor-1 modulates cardiac gene expression and exacerbates angiotensin II-induced cardiac hypertrophy Clin. Sci. 2020-01-27 [PMID: 31985010]
• Vodyanoy V, Pustovyy O, Globa L et al. Hemmule: A Novel Structure with the Properties of the Stem Cell Niche Int J Mol Sci 2020-01-18 [PMID: 31947705]
• Minna E, Brich S, Todoerti K et al. Cancer Associated Fibroblasts and Senescent Thyroid Cells in the Invasive Front of Thyroid Carcinoma Cancers (Basel) 2020-01-01 [PMID: 31906302] (IF/IHC, IHC-P, Human)
• Regoli M, Tosi GM, Neri G The Peculiar Pattern of Type IV Collagen Deposition in Epiretinal Membranes J. Histochem. Cytochem. 2019-12-20 [PMID: 31858878] (ICC/IF, Human)
• Liu Z, Qian M, Tang X, et al. Targeted regulation of fibroblast state by CRISPR-mediated CEBPA expression Respir. Res. 2019-12-11 [PMID: 31829168] (ICC/IF, Human)
• Aljohani H, Senbanjo LT, Chellaiah MA Methylsulfonylmethane increases osteogenesis and regulates the mineralization of the matrix by transglutaminase 2 in SHED cells PLoS ONE 2019-12-05 [PMID: 31805069] (WB, Human)
• Choi JH, Kim SM, Lee GH et al. Platyconic Acid A, Platycodi Radix-Derived Saponin, Suppresses TGF-1-induced Activation of Hepatic Stellate Cells via Blocking SMAD and Activating the PPAR Signaling Pathway Cells. 2019-11-29 [PMID: 31795488] (WB, Rat)
• Fu C, Lu Y, Williams MA, et al. Emergency myelopoiesis contributes to immune cell exhaustion and pulmonary vascular remodeling Br. J. Pharmacol. 2019-12-03 [PMID: 31793661] (WB, Mouse)
• Chen PY, Qin L, Li G et al. Endothelial TGF-beta signalling drives vascular inflammation and atherosclerosis Nat Metab 2019-09-01 [PMID: 31572976] (IF/IHC, Mouse)
• Benvenuto M, Mattera R, Miele MT et al. Effects of a natural multi-component compound formulation on the growth, morphology and extracellular matrix production of human adult dermal fibroblasts Exp Ther Med [PMID: 31572512]
• Balasubramanian M, Hobson E, Skae M et al. Developing pathways to clarify pathogenicity of unclassified variants in Osteogenesis Imperfecta genetic analysis Mol Genet Genomic Med 2019-09-30 [PMID: 31568717] (ICC/IF, WB, Human)
• Elrashidy RA, Liu G Long-term diabetes causes molecular alterations related to fibrosis and apoptosis in rat urinary bladder Exp. Mol. Pathol. 2019-08-31 [PMID: 31479659]
• Vidoni C, Ferraresi A, Secomandi E et al. Autophagy drives osteogenic differentiation of human gingival mesenchymal stem cells Cell Commun. Signal 2019-08-19 [PMID: 31426798] (ICC/IF, WB, Human)
• Sato K, Yoshizawa H, Seki T et al. Chemerin-9, a potent agonist of chemerin receptor (ChemR23), prevents atherogenesis Clin. Sci. 2019-08-30 [PMID: 31399499] (WB, Human)
• Suo H, Li L, Zhang C et al. Glucosamine-grafted methacrylated gelatin hydrogels as potential biomaterials for cartilage repair J. Biomed. Mater. Res. Part B Appl. Biomater. 2019-08-01 [PMID: 31369700]
• Guo Xiangjiang, Fereydooni Arash, Isaji Toshihiko et al. Inhibition of the Akt1-mTORC1 Axis Alters Venous Remodeling to Improve Arteriovenous Fistula Patency. Scientific Reports 2019-07-30 [PMID: 31363142] (IHC-P, Mouse)
• Xue YZ, Li ZJ, Liu WT et al. Down-regulation of lncRNA MALAT1 alleviates vascular lesion and vascular remodeling of rats with hypertension Aging (Albany NY) 2019-07-25 [PMID: 31343412] (WB, Rat)
• van de Vlekkert D, Demmers J, Nguyen XX et al. Excessive exosome release is the pathogenic pathway linking a lysosomal deficiency to generalized fibrosis Sci Adv 2019-07-01 [PMID: 31328155] (IF/IHC, ICC/IF, WB, Mouse)
• Obata T, Tsuchiya T, Akita S et al. Utilization of natural detergent potassium laurate for decellularization in lung bioengineering Tissue Eng Part C Methods 2019-07-18 [PMID: 31317819] (MiAr, Human)
• Xu L, Sharkey D, Cantley LG Tubular GM-CSF Promotes Late MCP-1/CCR2-Mediated Fibrosis and Inflammation after Ischemia/Reperfusion Injury J. Am. Soc. Nephrol. 2019-07-17 [PMID: 31315923] (WB, Mouse)
• Goldstein RL, Tsui JM, Runyan G et al. Photochemical Tissue Passivation Prevents Contracture of Full Thickness Wounds in Mice Lasers Surg Med 2019-07-06 [PMID: 31278757] (IF/IHC, Mouse)
• Hashimoto Y, Tsuchiya T, Doi R et al. Alteration of the extracellular matrix and alpha-gal antigens in the rat lung scaffold reseeded using human vascular and adipogenic stromal cells J Tissue Eng Regen Med 2019-07-02 [PMID: 31267683] (IF/IHC, Human, Rat)
• Lavin RC Exploiting Reporter Mycobacterium tuberculosis Strains to Illuminate Infection Heterogeneity in vivo and Identify New Antitubercular Compounds Thesis 2023-01-01
• Esteve D, Boulet N, Belles C et al. Lobular architecture of human adipose tissue defines the niche and fate of progenitor cells Nat Commun 2019-06-11 [PMID: 31186409] (IF, Human)
• Mehta N, Gava AL, Zhang D et al. Follistatin Protects Against Glomerular Mesangial Cell Apoptosis and Oxidative Stress to Ameliorate Chronic Kidney Disease Antioxid. Redox Signal. 2019-07-03 [PMID: 31184201] (WB)
• Shunsuke Kawai, Hiroyuki Yoshitomi, Junko Sunaga, Cantas Alev, Sanae Nagata, Megumi Nishio, Masataka Hada, Yuko Koyama, Maya Uemura, Kazuya Sekiguchi, Hirotsugu Maekawa, Makoto Ikeya, Sakura Tamaki, Yonghui Jin, Yuki Harada, Kenichi Fukiage, Taiji Adachi, Shuichi Matsuda, Junya Toguchida In vitro bone-like nodules generated from patient-derived iPSCs recapitulate pathological bone phenotypes. Nature biomedical engineering 2020-03-09 [PMID: 31182836]
• Nunzia Caporarello, Jeffrey A Meridew, Dakota L Jones, Qi Tan, Andrew J Haak, Kyoung M Choi, Logan J Manlove, Y S Prakash, Daniel J Tschumperlin, Giovanni Ligresti PGC1 alpha repression in IPF fibroblasts drives a pathologic metabolic, secretory and fibrogenic state Thorax 2019-08-01 [PMID: 31182654]
• Li Y, Wang W, Chao Y et al. CTRP13 attenuates vascular calcification by regulating Runx2 FASEB J. 2019-05-30 [PMID: 31145871]
• Huang C, Zhang X, Jiang L et al. CBX5/G9a/H3K9me-mediated gene repression is essential to fibroblast activation during lung fibrosis JCI Insight 2019-05-16 [PMID: 31095524] (Human, Mouse)
• Millar JC and Clark AF. Glucocorticoid Receptor Transactivation Is Required for Glucocorticoid-Induced Ocular Hypertension and Glaucoma. Invest Ophthalmol Vis Sci. 2019-05-01 [PMID: 31050723] (IHC-P, ICC/IF, Mouse)
• Legrand JMD, Chan AL, La HM et al. Nascent Lung Organoids Reveal Epithelium- and BMP-Mediated Suppression of Fibroblast Activation Am. J. Respir. Cell Mol. Biol. 2019-05-03 [PMID: 31050552] (IF/IHC, Human)
• Uehara M, Li X, Sheikhi A et al. Anti-IL-6 eluting immunomodulatory biomaterials prolong skin allograft survival. Sci Rep. 2019-04-25 [PMID: 31024011] (ICC/IF, Mouse)
  
  Details:
  Citation using the Non-Recombinant Monoclonal version of this antibody.
• Van Krieken R, Mehta N et al. Cell surface expression of 78-kDa glucose-regulated protein (GRP78) mediates diabetic nephropathy. J Biol Chem 2019-10-05 [PMID: 30914477] (PAGE, Rat)
• MacDonald JA, Takai Y, Ishihara O et al. Extracellular matrix signaling activates differentiation of adult ovary derived oogonial stem cells ina species-specific manner Fertil. Steril. 2019-04-01 [PMID: 30871765] (IHC-P, Mouse)
• Wang C, Xu W, An J et al. Poly(ADP-ribose) polymerase 1 accelerates vascular calcification by upregulating Runx2 Nat Commun 2019-03-13 [PMID: 30867423] (WB, Rat)
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  Details:
  Collagen I alpha 1 antibody used for IHC-P on human kidney allograft protocol biopsies (Fig 1)
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  Details:
  Collagen I alpha 1 antibody used for Far-Western blot analysis on cell-free supernatants (CFSs) from Aeromonas piscicola AH-3 and Aeromonas piscicola AH-3::colAh knockout mutants, with collagen type I as a positive control (Figure 1C).
• Gehmlich K, Dodd MS, William Allwood J et al. Changes in the cardiac metabolome caused by perhexiline treatment in a mouse model of hypertrophic cardiomyopathy. Mol Biosyst. 2014-12-01 [PMID: 25437646] (WB, Mouse)
  
  Details:
  Collagen I alpha 1 antibody used in WB for examining the Collagen 1 protein levels in perhexiline- and placebo-treated Mybpc3 KI mice compared to WT, with endogenous protein calsequestrin (CSQ) used as a normalization control. WB data is shown in Figure S4A.
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  Details:
  Collagen I antibody used for WB on protein extracts from rat's pancreatic stellate cells/ PSCs using PVDF membrane protein transfer and LI-COR -Odyssey detection. Figure 4A - type?I?collagen 150 kDa expression levels in Rat's PSCs treated or not with 48h of 10uM/L erlotinib, sorafenib or sunitinib.
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Reviews for Collagen I Antibody (NB600-408) (16)

Average Rating: 4.6

(Based on 16 reviews)

We have 16 reviews tested in 5 species: Human, Mouse, Rat, Feline, Other.

reviewed by: Federica Gnudi IHC-P Rat 05/05/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Rat Lung Tissue
• Species: Rat
• Lot: 47500

reviewed by: Verified Customer ICC Human 04/23/2021

Summary

• Application: Immunocytochemistry
• Sample Tested: chondrocytes
• Species: Human
• Lot: 44222

reviewed by: Verified Customer IHC-P Mouse 10/10/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse Pancreas
• Species: Mouse
• Lot: 42336

reviewed by: Verified Customer IHC-P Mouse 10/02/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Balb/3T3 mouse embryonic fibroblast cell line
• Species: Mouse
• Lot: 40267

reviewed by: Wangsheng Wang WB Human 05/30/2018

Summary

• Application: Western Blot
• Sample Tested: whole cell extract
• Species: Human
• Lot: 35820

reviewed by: Verified Customer IHC-P Mouse 10/25/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: IHC on parrafin sections (mouse)
• Species: Mouse
• Lot: 38594

reviewed by: Verified Customer WB Human 09/27/2017

Summary

• Application: Western Blot
• Sample Tested: Cell lysate from human trabecular meshwork
• Species: Human
• Lot: 38594

reviewed by: Verified Customer ICC Feline 06/23/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Adult small intestine
• Species: Feline
• Lot: 38594

Comments

• Comments: 4% PFA fixed feline tissue

reviewed by: Jakob Townsend IHC-P Rat 02/20/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Bone
• Species: Rat
• Lot: 37789

reviewed by: Verified Customer IHC-P Rat 07/20/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: rat colon
• Species: Rat
• Lot: 34015

reviewed by: Verified Customer WB Other 08/04/2015

Summary

• Application: Western Blot
• Species: Other

reviewed by: Verified Customer IF Human 10/02/2014

Summary

• Application: Immunofluorescence
• Sample Tested: primary human cardiac fibroblasts
• Species: Human
• Lot: 32011

reviewed by: Elizabeth Rendina-Ruedy WB Mouse 08/05/2013

Summary

• Application: Western Blot
• Sample Tested: Mouse Flushed Femur and whole cell lysate MC3T3
• Species: Mouse

reviewed by: Verified Customer IHC-Fr Human 06/18/2013

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: HaCaT-ras A5-RT3 (SCC) tumor sections
• Species: Human

reviewed by: Nathan Weidenhamer ICC Human 02/04/2013

Summary

• Application: Immunocytochemistry
• Sample Tested: human dermal fibroblast derived cell sheet
• Species: Human

reviewed by: Verified Customer WB Human 09/04/2012

Summary

• Application: Western Blot
• Sample Tested: Fibroblast Cell Media
• Species: Human

FAQs for Collagen I Antibody (NB600-408). (Showing 1 - 2 of 2 FAQs).

1. Could you please advise if this antibody works for IHC-P application using bone tissues?
   • NB600-408 has been tested and validated for use in the following applications and species: Western blot, ELISA, IP and IHC-Paraffin in human, mouse, rat and bovine. We have not specifically tested bone tissue.
2. Do you follow standard Western procedures with this collagen Ab or can the protein not be denatured?
   • Since we have not posted any special instruction for using this antibody in WB on our web, you could use the standard WB protocol. Although this collagen I a 1 is a huge protein (Uniprot site), the structure is relatively simple; with few modified amino acids and glycosylations. The detection of this protein with SDS-PAGE (denaturing gel) will ensure the molecular size of the protein could be accurately detected and confirmed, even though the glycosylations may alter the true MW. The manufacture of NB600-408 was carried out by the injections of rabbit with the immunogens derived from native collagen Type I fragments derived from human and bovine placenta after the limited pepsin digestions and selective salt precipitations. Therefore this antibody should recognize the native, undenatured collagen 1 alpha 1. If using the undenatured lysates in WB, you should expect to detect a very large protein complexes on your gel/WB, as the collagen I a 1 interacts with itself and/or other components in the extracellular matrices; but our WB protocol should be able to serve the propose.

Bioinformatics

• Gene Symbol: COL1A1
• Entrez:
  • 282187(Bovine)
  • 1277(Human)
  • 29393(Rat)
• Uniprot:
  • P02452()
  • P02452()
  • P02452()
  • P02452()
  • P02452()
  • P02453(Bovine)