Rat Collagen IV alpha 1 ELISA Kit (Colorimetric)

ELISA: Rat Collagen IV alpha 1 ELISA Kit (Colorimetric) [NBP2-75872] - Samples were spiked with high concentrations of Rat Collagen IV alpha 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Collagen IV alpha 1 ELISA Kit (Colorimetric) [NBP2-75872] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL

Rat Collagen IV alpha 1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat COL4a1 in samples. No significant cross-reactivity or interference between Rat COL4a1 and analogues was observed.
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.14%
• Intra-Assay: CV% < 4.91%
• Spike Recovery: 85-109%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: COL4A1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Collagen IV alpha 1 ELISA Kit (Colorimetric)

• alpha-1 chain
• Arresten
• COL4A1 NC1 domain
• COL4A1
• collagen alpha-1(IV) chain
• Collagen IV alpha 1
• collagen IV, alpha-1 polypeptide
• collagen, type IV, alpha 1
• Collagen-4
• EC 3.4.23
• EC 6.3.1.2

Background

Collagen IV is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: COL4A1