Collagen V Antibody - BSA Free

Immunohistochemistry-Paraffin: Collagen V Antibody [NB120-7046] - Analysis using the Biotin conjugate of NB120-7046. collagen V antibody 1:200, 45 min RT showed strong staining in FFPE sections of human lung (left) with strong staining within alveoli, vessels, and in connective tissue spaces; and placenta (right) with strong staining observed in stromal and connective tissue spaces and vessel walls. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval.

anti collagen V antibody ( Lot 22063, 1:200, 45 min RT) showed strong staining in FFPE sections of human lung (left) with strong staining within alveoli, vessels, and in connective tissue spaces; and placenta (right) with strong staining observed in stromal and connective tissue spaces and vessel walls. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA

• Reactivity: Hu, Bv
• Applications: WB, DB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Collagen V Antibody - BSA Free Summary

• Description: This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
  
  Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Immunogen: Collagen V from human and bovine placenta (Uniprot: P25940)
• Localization: Extracellular matrix
• Specificity: Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen V and has negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL5A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:5000-1:50000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:100
  • Western Blot 1:1000-1:10000
• Application Notes: This product has been tested by dot blot and IHC and is suitable by indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.
  
  Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27092719).

Reactivity Notes

This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Collagen V Antibody - BSA Free

• alpha 1 type V collagen
• Collagen 5
• collagen alpha-1(V) chain
• collagen, type V, alpha 1
• Collagen-5
• EC 2.7.7.6
• EC 6.1.1

Background

This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Collagen V Antibody (NB120-7046)(1)

Publication using NB120-7046

• Zhou C, Lei F, Chodosh J, Paschalis EI. The Role of Titanium Surface Microtopography on Adhesion, Proliferation, Transformation, and Matrix Deposition of Corneal Cells. Invest. Ophthalmol. Vis. Sci. 2016-04-01 [PMID: 27092719] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: COL5A1
• Entrez:
  • 1289(Human)
• Uniprot:
  • P05997(Human)