Complement C3d Antibody (E28-P)

Immunohistochemistry-Paraffin: Complement C3d Antibody (E28-P) [NBP1-79074] - Formalin-fixed and paraffin-embedded human skin biopsy (4 um) from lesion of the early pemphigus vulgaris (without blister formation), stained with anti-C3d complement antibody shows strong positive intraepidermal intercellular immunostaining.

• Reactivity: Hu
• Applications: IHC
• Clone: E28-P
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Complement C3d Antibody (E28-P) Summary

• Description: This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
• Immunogen: Peptide derived from N-terminal sequence of human Complement C3d fragment.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: C3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:200

Reactivity Notes

Mouse reactivity may be low or non-existant. Initial results based on customer testing. Please see our product reviews for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 20mM Tris-HCl (pH 8.0) and 20mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Alternate Names for Complement C3d Antibody (E28-P)

• AHUS5
• ARMD9
• ASP
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
• complement C3
• complement component 3
• complement component C3
• CPAMD1acylation-stimulating protein cleavage product

Background

C3d is a fragment of the major complement protein C3, C3d is formed as a result of the deactivation of C3b.Factor I in the presence of cofactors cleaves C3b to form iC3b and C3f, Factor I and/or other proteasesthen cleaves iC3b to form C3c and C3dg. C3dg is then further cleaved by tryptic enzymes to C3g andC3d. The C3 fragments are then eliminated from circulation by phagocytic cells in the liver. C3d has amolecular weight of 34kDa. Normally C3d is present in trace amounts in plasma, however, in diseasestates where rapid complement consumption is occurring levels of C3d can be elevated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement C3d Antibody (NBP1-79074)(1)

Publication using NBP1-79074

• Abu-Humaidan AHA, Ahmad FM, Awajan D et al. Anti-SARS-Cov-2 S-RBD IgG Formed after BNT162b2 Vaccination Can Bind C1q and Activate Complement Journal of immunology research 2022-12-17 [PMID: 36573216] (ELISA, Human)

Review for Complement C3d Antibody (NBP1-79074) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 12/20/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Diseased kidney
• Species: Mouse

Comments

• Comments: Note: This customer submitted a review in an untested species (mouse)
  
  I tried the antibody NBP1-79074 at a dilution of 1:10 using three different epitope retrieval methods: a) HiER with citrate, b) HiER in alkaline Tris, and c) enzymatic pre-treatment with Proteinase K. I saw no signal under any conditions.

Bioinformatics

• Gene Symbol: C3
• Uniprot:
  • P01024()