COP1 Antibody (1E4) - Azide and BSA Free

Western Blot: COP1 Antibody (1E4) [H00064326-M01] - RFWD2 monoclonal antibody (M01), clone 1E4 Analysis of RFWD2 expression in NIH/3T3.

Immunocytochemistry/Immunofluorescence: COP1 Antibody (1E4) [H00064326-M01] - Analysis of monoclonal antibody to RFWD2 on NIH/3T3 cell. Antibody concentration 10 ug/ml.

Western Blot: COP1 Antibody (1E4) [H00064326-M01] - RFWD2 monoclonal antibody (M01), clone 1E4. Analysis of RFWD2 expression in A-431.

Western Blot: COP1 Antibody (1E4) [H00064326-M01] - RFWD2 monoclonal antibody (M01), clone 1E4. Analysis of RFWD2 expression in Jurkat.

Western Blot: COP1 Antibody (1E4) [H00064326-M01] - RFWD2 monoclonal antibody (M01), clone 1E4. Analysis of RFWD2 expression in K-562.

Sandwich ELISA: COP1 Antibody (1E4) [H00064326-M01] - Detection limit for recombinant GST tagged RFWD2 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 1E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

COP1 Antibody (1E4) - Azide and BSA Free Summary

• Immunogen: RFWD2 (NP_071902, 632 a.a. ~ 731 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLELV
• Specificity: RFWD2 - ring finger and WD repeat domain 2
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: COP1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for COP1 Antibody (1E4) - Azide and BSA Free

• Constitutive photomorphogenesis protein 1 homolog
• constitutive photomorphogenic protein (COP1)
• COP1RNF200FLJ10416
• E3 ubiquitin-protein ligase RFWD2
• EC 6.3.2.-
• hCOP1
• putative ubiquitin ligase COP1
• ring finger and WD repeat domain 2
• RING finger and WD repeat domain protein 2
• RING finger protein 200

Background

COP1 is an E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. It is directly involved in p53 ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. It ubiquitinates p53 independently of MDM2 or RCHY1. It probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, where the ubiquitin ligase activity is mediated by RBX1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for COP1 Antibody (H00064326-M01). (Showing 1 - 1 of 1 FAQ).

1. Could you let us know whether this antibody has been mentioned in a publication so that we can look at those images? Moreover, could you please tell us whether the immunoblot presented on you website shows the labeling of the endogenous RFWD2 from 3T3 cells or whether a lysate of cells transfected with an RFWD2 expression vector has been used for blotting?
   • This product has not yet been mentioned in any publications that we are aware of. And in regards to the WB image, this image was generated with 3T3 cells showing the staining of endogenous COP1 / RFWD2.

Bioinformatics

• Gene Symbol: COP1
• Entrez:
  • 64326(Human)
• OMIM:
  • 608067(Human)
• Uniprot:
  • NP_071902(Human)