COX-1 Assay Kit (Luminescent)

COX-1 Assay Kit (Fluorometric) [KA0323] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

COX-1 Assay Kit (Fluorometric) [KA0323] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Product Discontinued

COX-1 Assay Kit (Luminescent) Summary

• Description: Quality control test: Standard curve COX Activity Kit is for the quantitative determination of Cyclooxygenase activity in biological samples.
• Standard Curve Range: 7.5 to 60 Units/mL
• Sample Volume: 50 uL
• Kit Type: Assay Kit (Luminescent)
• Gene: PTGS1

Applications/Dilutions

• Dilutions:
  • Functional

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for COX-1 Assay Kit (Luminescent)

• COX1
• COX-1
• COX1PGG/HS
• COX3
• cyclooxygenase-1
• EC 1.14.99.1
• PCOX1
• PGH synthase 1
• PGHS1
• PGHS-1PHS1
• PHS 1
• prostaglandin G/H synthase 1
• prostaglandin G/H synthase and cyclooxygenase
• Prostaglandin H2 synthase 1
• prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase andcyclooxygenase)
• Prostaglandin-endoperoxide synthase 1
• PTGHS
• PTGS1

Background

Cyclooxygenase (COX, also known as Prostaglandin G/H synthase) is a membrane bound enzyme responsible for the oxidation of arachidonic acid to Prostaglandin G2 (PGG2) and the subsequent reduction of PGG2 to PGH2. The conversion is shown below. These reactions are the first steps in the formation of a variety of prostanoids. COX has been shown to be expressed in at least two different isoforms: a constitutively expressed form, COX-I, and an inducible form, COX-II. COX-I is thought to regulate a number of 'housekeeping' functions, such as vascular hemostasis, renal blood flow, and maintenance of glomerular function .Inflammation mediators such as growth factors, cytokines and endotoxin induce COX-II expression in a number of cellular systems.The effect of various non-steroidal anti-inflammatory drugs (NSAIDs) on the activity of COX-I and -II is an area of considerable interest. Some methods to determine COX activity involve procedures such as measuring uptake of oxygen using an oxygraph, measuring the conversion of radioactive arachidonic acid, or measuring the prostaglandins formed from PGH2 (such as determining PGE2 using immunoassays). Most of these methods are complex, time consuming, and are prone to interferences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for COX-1 Kit (KA0323)(3)

Publications using KA0323

• Ana G, Adriana V, Marcia R et al. The impact of cryopreservation in signature markers and immunomodulatory profile of tendon and ligament derived cells. J Cell Physiol. 2021-08-08 [PMID: 34368976]
• Adriana V, Ana G, Marcia R et al. Human tendon-derived cell sheets created by magnetic force-based tissue engineering hold tenogenic and immunomodulatory potential. Acta Biomater. 2021-06-27 [PMID: 34192569]
• Dandan C, Yu H, Xingjun C et al. MiR-210-3p Enhances Cardiomyocyte Apoptosis and Mitochondrial Dysfunction by Targeting the NDUFA4 Gene in Sepsis-Induced Myocardial Dysfunction. Int Heart J. 2021-05-15 [PMID: 33994501]

FAQs for COX-1 Kit (KA0323). (Showing 1 - 1 of 1 FAQs).

1. Please could you tell me if Novus Biologicals has an active COX1 Recombinant Protein?
   • Unfortunately we do not currently have any COX1 recombinant proteins that are expected to be active.

Bioinformatics

• Gene Symbol: PTGS1