COX4 Antibody - BSA Free

Western Blot: COX4 Antibody - BSA Free [NB110-39115] - Increased mitochondrial localization of CREB under hypoxiaA. Mitochondria were sub fractionated into outer membrane (OM), inter membrane space (IMS), inner membrane (IM) and mitochondrial matrix (MM). The purity of the fractions was analysed with marker proteins: TOM20 for OM, AIF for IMS, COX4 for IM, PDK4 for MM. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/10474/text/) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: COX4 Antibody - BSA Free [NB110-39115] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-COX4 at 10 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: COX4 Antibody - BSA Free [NB110-39115] - Analysis of COX4 in human breast cancer using DAB with hematoxylin counterstain.

Western Blot: COX4 Antibody - BSA Free [NB110-39115] - The mRNA and protein expression of cytochrome c oxidase 4 (COX4), toll-like receptor (TLR) 9, myeloid differentiation factor 88 (MyD88), and nuclear factor (NF)-kB in lung tissues from spontaneous breathing group (CON group), normal tidal volume (NTV) group, high tidal volume (HTV) group, chloroquine (CQ) pretreatment upon HTV stimulation group (HTV + CQ). Levels of COX4, TLR9, MyD88, and NF-kB protein by Western blot. Fold expression for target genes was normalized to that measured for the beta-actin gene. Both of these experiments were in triplicate. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30018615/) licensed under a CC-BY license.

Western Blot: COX4 Antibody - BSA Free [NB110-39115] - Analysis of COX4 in the following cell lysates: 1. HeLa, 2. Ntera, 3. A431, 4. HepG2, 5. MCF7 and 6. 3T3.

Western Blot: COX4 Antibody - BSA Free [NB110-39115] - Analysis of COX4 in porcine whole skeletal muscle lysate using anti-COX4 antibody. Image from verified customer review.

Western Blot: COX4 Antibody - BSA Free [NB110-39115] - Detection of COX4-1 isoform using COX4 antibody NB110-39115. Lane 1: HEK 293 with empty vector, Lane 2: HEK 293 with flag-tagged human COX4-1, Lane 3: HEK 293 with flag-tagged human COX4-2. Photo courtesy of Ryo Fukuda, G. Semenza lab. Johns Hopkins, SOM

Western Blot: COX4 Antibody - BSA Free [NB110-39115] - Total protein from HeLa, 3T3 and PC12 was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-COX4 in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: COX4 Antibody - BSA Free [NB110-39115] - COX4 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunocytochemistry/Immunofluorescence: COX4 Antibody - BSA Free [NB110-39115] - Analysis of HeLa cells using COX4 antibody (NB110-39115, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Immunocytochemistry: COX4 Antibody - BSA Free [NB110-39115] - Analysis of methanol fixed drosophila embryo using 1:200 dilution of COX4 antibody. The signal was developed using AF488 conjugated Donkey anti-Rabbit IgG (H+L) secondary antibody and the sections were further counterstained for tubulin and DAPI. The antibody generated a specific staining of COX4 in mitochondria near mitotic spindles at early stage of embryo development. Image from verified customer review.

Simple Western: COX4 Antibody - BSA Free [NB110-39115] - Image shows a specific band for COX4 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: COX4 Antibody - BSA Free [NB110-39115] - The expression levels of microtubule protein light chain 3 (LC3B), PTEN inducing putative kinase 1 (PINK1), Parkin, mitofusin 1 (Mfn1), toll-like receptor (TLR) 9, cytochrome c oxidase 4 (COX4), myeloid differentiation factor 88 (MyD88), & nuclear factor (NF)-kappa B in lung tissues from animals with spontaneous breathing (CON) or mechanical ventilation at high tidal volume (HTV) with saline, antimycin A (AmA) or cyclosporine A (CsA). (A) Levels of PINK1, Parkin, & Mfn1 mRNA. (B) Levels of LC3B, PINK1, Parkin, & Mfn1 protein by Western blot. (C) Relative expression of LC3B-II/LC3B-I & PINK1 protein. (D) Relative expression of Parkin & Mfn1 protein. (E) Levels of TLR9 & COX4 mRNA. (F) Levels of MyD88 & nuclear factor-kappa B (NF-kappa B) mRNA. (G) Levels of TLR9, COX4, MyD88, & NF-kappa B protein by Western blot. (H) Relative expression of TLR9 & COX4 protein. (I) Relative expression of MyD88 & NF-kappa B protein. Fold expression for target genes was normalized to that measured for the beta -actin gene. Both of these experiments were in triplicate. aP < 0.05 vs. CON group;bP < 0.05 vs. HTV group; & cP < 0.05 vs. AmA group. Image collected & cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Po, Bv, Dr, I, Ma-Op, Pm
• Applications: WB, Simple Western, ICC/IF, IHC, ChIP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

COX4 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal region of human COX IV isoform 1 (within residues 1-100). [Swiss-Prot# P13073]
• Localization: Mitochondrion, mito inner membrane
• Marker: Mitochondria Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COX4I1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence 1:40
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Knockdown Validated reported in scientific literature (PMID 31655343)
  • Simple Western 1:25
  • Western Blot 1:2000
• Application Notes: In Western Blot, a band is seen ~19.5 kDa representing COX IV. In ICC/IF, mitochondrion staining was observed in HeLa cells. In IHC-P, staining is observed in the cytoplasm and mitochondria of human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Higher dilutions may be needed for mitochondrial membrane enriched preparations. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, titrated to saturation using various models; separated by Size; antibody dilution of 1:25, 75 ug/mL; apparent MW was 22 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  293 Whole Cell Lysate
  HeLa Whole Cell Lysate
  COX4 Overexpression Lysate

• Control Peptide:

  COX4 Protein (NB110-39115PEP)

Reactivity Notes

Opossum reactivity reported in scientific literature (PMID: 28720662).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for COX4 Antibody - BSA Free

• COX IV-1
• COX4
• COX4-1
• COX4COXIV
• COX4I2
• Cytochrome c oxidase polypeptide IV
• cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• cytochrome c oxidase subunit IV isoform 1cytochrome c oxidase subunit IV
• MGC72016

Background

Cytochrome C Oxidase (COX), which is located in the inner mitochondrial membrane, is the terminal enzyme complex of the mitochondrial electron transport chain. It collects electrons that are transferred from reduced Cytochrome C and donates them to molecular oxygen, which is then reduced to water.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for COX4 Antibody (NB110-39115)(19)

Publications using NB110-39115

• Zhao Y, Liu Y, Zhao G et al. Myeloid BAF60a deficiency alters metabolic homeostasis and exacerbates atherosclerosis Cell reports 2023-09-26 [PMID: 37768825] (WB, Mouse)
• Papadaki V, Erpapazoglou Z, Kokkori M et al. IQGAP1 mediates the communication between the nucleus and the mitochondria via NDUFS4 alternative splicing NAR Cancer 2023-08-24 [PMID: 37636315] (Western Blot, Human, Mouse)
• Jing X, Wu J, Dong C et al. COVID-19 instigates adipose browning and atrophy through VEGF in small mammals Nature metabolism 2022-12-01 [PMID: 36482111] (IHC-P, Mouse)
• Kaseder M, Schmid N, Eubler K et al. Evidence of a role for cAMP in mitochondrial regulation in ovarian granulosa cells Molecular human reproduction 2022-08-09 [PMID: 35944223]
• Treidel LA, Quintanilla Ramirez GS, Chung DJ et al. Selection on dispersal drives evolution of metabolic capacities for energy production in female wing-polymorphic sand field crickets, Gryllus firmus Journal of evolutionary biology 2022-03-07 [PMID: 35255175] (WB, Insect)
  
  Details:
  Gryllus firmus
• Julia María Torres-Velarde, Sree Rohit Raj Kolora, Jane I Khudyakov, Daniel E Crocker, Peter H Sudmant, José Pablo Vázquez-Medina Elephant seal muscle cells adapt to sustained glucocorticoid exposure by shifting their metabolic phenotype. American journal of physiology. Regulatory, integrative and comparative physiology 2021-09-16 [PMID: 34260302]
• Jingwen Zhang, Zhibin He, Julia Fedorova, Cole Logan, Lauryn Bates, Kayla Davitt, Van Le, Jiayuan Murphy, Melissa Li, Mingyi Wang, Edward G. Lakatta, Di Ren, Ji Li Alterations in mitochondrial dynamics with age‐related Sirtuin1/Sirtuin3 deficiency impair cardiomyocyte contractility Aging Cell 2021-07-03 [PMID: 34216536]
• Burmakin M, Fasching A, Kobayashi H et al. Pharmacological HIF-PHD inhibition reduces renovascular resistance and increases glomerular filtration by stimulating nitric oxide generation Acta physiologica (Oxford, England) 2021-04-26 [PMID: 33900001] (IF/IHC, Rat)
• Chung YJ, Swietach P, Curtis MK et al. Iron-Deficiency Anemia Results in Transcriptional and Metabolic Remodeling in the Heart Toward a Glycolytic Phenotype Frontiers in Cardiovascular Medicine 2021-01-21 [PMID: 33553263] (Western Blot, Human, Mouse)
• Madhu V, Boneski PK, Silagi E et al. Hypoxic Regulation of Mitochondrial Metabolism and Mitophagy in Nucleus Pulposus Cells Is Dependent on HIF-1?-BNIP3 Axis Journal of Bone and Mineral Research 2020-08-01 [PMID: 32251541] (Western Blot, Human, Mouse)
• Huang CY, Hsu LH, Chen CY et al. Inhibition of Alternative Cancer Cell Metabolism of EGFR Mutated Non-Small Cell Lung Cancer Serves as a Potential Therapeutic Strategy Cancers (Basel) 2020-01-10 [PMID: 31936895] (WB, Human)
• Moskal N, Riccio V, Bashkurov M et al. ROCK inhibitors upregulate the neuroprotective Parkin-mediated mitophagy pathway Nat Commun 2020-01-03 [PMID: 31900402]
• Du XK, Ge WY, Jing R, Pan LH Necroptosis in pulmonary macrophages mediates lipopolysaccharide-induced lung inflammatory injury by activating ZBP-1 Int. Immunopharmacol. 2019-10-23 [PMID: 31655343] (WB, KD, Mouse)
• Violitzi F, Perivolidi VI, Thireou T et al. Mapping interactome networks of DNAJC11, a novel mitochondrial protein causing neuromuscular pathology in mice J. Proteome Res. [PMID: 31550165] (Human)
• Ren, Q;Gliozzi, ML;Rittenhouse, NL;Edmunds, LR;Rbaibi, Y;Locker, JD;Poholek, AC;Jurczak, MJ;Baty, CJ;Weisz, OA; Shear stress and oxygen availability drive differential changes in OK proximal tubule cell metabolism and endocytosis Traffic 2019-04-15 [PMID: 30989771] (WB)
• Lin JY, Jing R, Lin F et al. High Tidal Volume Induces Mitochondria Damage and Releases Mitochondrial DNA to Aggravate the Ventilator-Induced Lung Injury. Front Immunol. 2018-07-03 [PMID: 30018615] (WB, Rat)
• Long KR, Shipman KE, Rbaibi Y et al. Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway Mol Biol Cell. 2017-09-14 [PMID: 28720662] (WB, Opossum)
• Morten KJ, Badder L, Knowles HJ. Differential regulation of HIF-mediated pathways increases mitochondrial metabolism and ATP production in hypoxic osteoclasts. J Pathol. 2013-04-01 [PMID: 23303559] (WB, Human)
• Zan H, Zhang J, Al-Qahtani A et al. Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions. Mol Immunol 2011-01-01 [PMID: 21111482] (WB, Mouse, Human)

Reviews for COX4 Antibody (NB110-39115) (4)

Average Rating: 4.5

(Based on 4 reviews)

We have 4 reviews tested in 3 species: Human, Drosophila, Other.

reviewed by: ilaria drago WB Drosophila 06/26/2017

Summary

• Application: Western Blot
• Sample Tested: 20 ug whole body lysate
• Species: Drosophila

Comments

• Comments: 20 ug whole body lysate run in a 12% gel.
  Antibody used 1:4000

reviewed by: Verified Customer ICC Other 10/29/2015

Summary

• Application: Immunocytochemistry
• Species: Other

reviewed by: Verified Customer WB Other 04/23/2015

Summary

• Application: Western Blot
• Sample Tested: Whole Skeletal Muscle Lysate
• Species: Other
• Lot: B-1

reviewed by: Bryan Tinsley ICC Human 06/16/2014

Summary

• Application: Immunocytochemistry
• Species: Human

Bioinformatics

• Gene Symbol: COX4I1