CRIPTO Antibody

Western Blot: CRIPTO Antibody [NB100-1597] - Analysis of Cripto in MDA-MB231 lysates.

Immunocytochemistry/Immunofluorescence: CRIPTO Antibody [NB100-1597] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue) and tubulin was stained with alpha tubulin (red).

Flow Cytometry: CRIPTO Antibody [NB100-1597] - An intracellular stain was performed on HeLa with NB100-1597 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.

Product Discontinued

CRIPTO Antibody Summary

• Immunogen: A synthetic peptide made to an internal portion of human Cripto1 (between residues 100-150). [UniProt# P13385]
• Localization: Membrane-bound
• Marker: Embryonic Stem Cell Marker
• Predicted Species: Mouse (93%), Zebrafish (93%), Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TDGF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100
  • Western Blot 1:500-1:2000
• Application Notes: This Cripto1 antibody is useful in Immunocytochemistry/Immunofluorescence, Flow Cytometry, and Western blot, In Western blot a band is seen ~18 kDa which is the core form of Cripto1. In ICC/IF, cytoplasmic and membrane staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  CRIPTO Overexpression Lysate

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: mouse (93%), cow (93%), and zebrafish (93%). There is no significant homology with any other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CRIPTO Antibody

• CFC-2
• CR
• Cripto
• Cripto-1 growth factor
• Cripto-1
• CRIPTOCRGF
• Epidermal growth factor-like cripto protein CR1
• TDGF1
• TDGF-1
• teratocarcinoma-derived growth factor 1

Background

Cripto-1 (teratocarcinoma-derived growth factor-1) is the original member of EGF-cripto-1-FRL-1-cryptic (CFC) family of vertebrate signaling molecules which is essential for embryonic development and it implicates in carcinogenesis also. Cripto1 functions as a co-receptor for NODAL (TGF-beta member) and is essential for mesoderm as well as endoderm formation and anterior-posterior and left-right axis establishment. After executing key functions during embryonic development, it dramatically disappears in adult tissues (except stem cells). Cripto-1 modulate signaling of TGF-beta family members (NODAL, GDF-1/-3, Activin, TGF-beta1 etc), and activate c-src/MAPK/AKT pathway in a Glypican-1/GRP78-dependent manner, and cross-talks with erbB4, Wnt/beta-catenin, NOTCH, Caveolin-1, and Apelin/putative receptor protein related to angiotensin-type I receptor pathways. During embryonic development, Cripto-1 together with Nodal controls cell migration and EMT, whereas, postnatally, it regulates the branching morphogenesis of mammary gland. Moreover, in adult life it gets re -expressed in several type of tumors wherein it promotes cell proliferation, migration, invasion, EMT, and angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRIPTO Antibody (NB100-1597)(1)

Publication using NB100-1597

• Calle A, Toribio-Serrano V, YANez-MO M, RamIrez M Embryonic trophectoderm secretomics reveals chemotactic migration and intercellular communication of endometrial and circulating MSCs in embryonic implantation Int J Mol Sci 2021-06-02 [PMID: 34073234]

Bioinformatics

• Gene Symbol: TDGF1
• Entrez:
  • 6997(Human)
• Uniprot:
  • P13385()