PMEL17/SILV Antibody (HMB45)

Western Blot: PMEL17/SILV Antibody (HMB45) [NBP2-44520] - Western Blot Analysis of COLO-38 cell lysate using PMEL17/SILV antibody (HMB45).

Immunohistochemistry: PMEL17/SILV Antibody (HMB45) [NBP2-44520] - PMEL17 (red) was detected in human skin (melanoma) using PMEL17-PE antibody (1:200) in PBS for 1 hour. Nuclei were stained with DAPI (blue). Image from a verified customer review. Image using the PE format of this antibody.

Flow (Intracellular): PMEL17/SILV Antibody (HMB45) [NBP2-44520] - An intracellular stain was performed on SK-MEL-28 cells with PMEL17/SILV Antibody [HMB45] NBP2-34638B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals). Image using the Biotin format of this antibody.

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (HMB45) [NBP2-44520] - Formalin-fixed, paraffin-embedded human melanoma stained with gp100/Melanosome Monoclonal Antibody (HMB45).

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (HMB45) [NBP2-44520] - Formalin-fixed, paraffin-embedded human testis stained with gp100/Melanosome Monoclonal Antibody (HMB45).

Flow (Intracellular): PMEL17/SILV Antibody (HMB45) [NBP2-44520] - Flow Cytometry: PMEL17/SILV Antibody (HMB45) - Azide and BSA Free [NBP2-34638] - An intracellular stain was performed on SK-MEL-28 cells with PMEL17/SILV Antibody (HMB45) NBP2-34638PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP. Image using the Azide and BSA Free form of this antibody.

• Reactivity: Hu, Ca(-), Rt(-)
• Applications: WB, Flow, ICC/IF, IHC, Func, ICC/IF
• Clone: HMB45
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PMEL17/SILV Antibody (HMB45) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34638)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Extract of pigmented melanoma metastases from lymph nodes
• Localization: Cytoplasmic
• Marker: Melanoma Marker
• Specificity: The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (6038kDa fragments. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This monoclonal antibody reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This monoclonal antibody stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This monoclonal antibody also stains Angiomyolipoma (PEComa).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PMEL
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Multiplex Immunoassay
  • Western Blot 2-4 ug/ml
• Application Notes: Use in Multiplex Immunoassay reported in scientific literature (PMID: 31942075).
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
  Use in Western blot reported in scientific literature (PMID: 30561643).

Reactivity Notes

Does not react with Canine or Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for PMEL17/SILV Antibody (HMB45)

• D12S53EP1
• Gp100
• ME20
• ME20M
• ME20-M
• melanocyte protein mel 17
• Melanocyte protein Pmel 17
• Melanocytes lineage-specific antigen GP100
• Melanoma-associated ME20 antigen
• melanosomal matrix protein17
• PMEL
• PMEL17
• PMEL17P100
• premelanosome proteinME20M
• SI
• SIL
• SILV
• silver (mouse homolog) like
• silver homolog (mouse)
• Silver locus protein homolog
• silver, mouse, homolog of
• SILVPmel17

Background

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PMEL17/SILV Antibody (NBP2-44520)(7)

Publications using NBP2-44520

• Hodges E, Chrystal P, Footz T et al. Disrupting the Repeat Domain of Premelanosome Protein (PMEL) Produces Dysamyloidosis and Dystrophic Ocular Pigment Reflective of Pigmentary Glaucoma International Journal of Molecular Sciences 2023-09-22 (WB, Human)
• Elizabeth D. Hodges, Paul W. Chrystal, Tim Footz, Lance P. Doucette, Nicole C. L. Noel, Zixuan Li, Michael A. Walter, W. Ted Allison, Paola Cicatiello Disrupting the Repeat Domain of Premelanosome Protein (PMEL) Produces Dysamyloidosis and Dystrophic Ocular Pigment Reflective of Pigmentary Glaucoma International Journal of Molecular Sciences 2023-09-22 [PMID: 37833870]
• Kamil Kruczek, Zepeng Qu, Emily Welby, Hiroko Shimada, Suja Hiriyanna, Milton A. English, Wadih M. Zein, Brian P. Brooks, Anand Swaroop In vitro modeling and rescue of ciliopathy associated with IQCB1/NPHP5 mutations using patient-derived cells Stem Cell Reports 2022-09-08 [PMID: 36084637]
• JOR Hernandez, X Wang, M Vazquez-Se, M Lopez-Marf, MF Sobral-Rey, A Moran-Horo, M Sundberg, DO Lopez-Cant, CK Probst, GU Ruiz-Espar, K Giannikou, R Abdi, EP Henske, DJ Kwiatkowsk, M Sahin, DR Lemos A tissue-bioengineering strategy for modeling rare human kidney diseases in vivo Nature Communications, 2021-11-11;12(1):6496. 2021-11-11 [PMID: 34764250] (FLOW)
• Phelan MA, Kruczek K, Wilson JP et al. Soy protein nanofiber scaffolds for uniform maturation of hiPSC-derived retinal pigment epithelium Tissue Eng Part C Methods 2020-07-07 [PMID: 32635833]
• Helmink BA, Reddy SM, Gao J et al. B cells and tertiary lymphoid structures promote immunotherapy response Nature. [PMID: 31942075] (MI, Human)
• Lahola-Chomiak AA, Footz T, Nguyen-Phuoc K et al. Non-Synonymous variants in Premelanosome Protein (PMEL) cause ocular pigment dispersion and pigmentary glaucoma Hum. Mol. Genet. 2018-12-17 [PMID: 30561643] (WB, Human)

Bioinformatics

• Gene Symbol: PMEL
• Uniprot:
  • P40967(Human)