CTCF Antibody - BSA Free

Immunoprecipitation: CTCF Antibody [NB500-175] - Immunoprecipitation of Human CTCF. Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded/lane) from MCF7 cells. Antibodies: Affinity purified rabbit anti-CTCF antibody NB500-175 used at 3 mcg/mg lysate for IP. Human CTCF was also immunoprecipitated using affinity purified rabbit anti-CTCF antibodies NB500-177 and NB500-194 CTCF was blotted using NB500-177 at 0.03 mcg/ml. Detection: Chemiluminescence with exposure times of 10 seconds.

• Reactivity: Hu
• Applications: IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CTCF Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 150 and 200 of human CCCTC-binding factor using the numbering given in entry NP_006556.1 (GeneID 10664).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CTCF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: NB 500-175 may be used for immunoprecipitation. Not recommended for Western Blot, use NB 500-177. *The investigator should determine the optimal working dilution for a specific application.
• Control:

  IMP2/IGF2BP2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CTCF Antibody - BSA Free

• 11 zinc finger transcriptional repressor
• 11-zinc finger protein
• 11-Zinc Finger Protein2
• CCCTC-binding factor (zinc finger protein)
• CCCTC-binding Factor
• CTCF
• CTCFL paralog
• transcriptional repressor CTCF

Background

The vertebrate insulator protein, CCCTC binding factor (CTCF) is a highly conserved, ubiquitously expressed DNA-binding protein that has widespread capabilities in gene regulation. CTCF contains 11 zinc finger motifs and it is thought that CTCF uses different combinations of zinc fingers to recognise a variety of specific sequences on the DNA to control several important genes. CTCF plays dual roles in cell growth regulatory processes and epigenetic functions. CTCF is a negative regulator of cell proliferation and is located in a region of the chromosome that is frequently deleted in human cancers. The CTCF protein is altered in some breast and prostate tumours. Substitutions from one amino acid to another in the DNA-binding domain change the whole spectrum of the genes regulated by CTCF which can result in cancer development. CTCF was first identified as one of the nuclear factors binding to the regulatory regions of the c-myc gene. Further study of this protein revealed roles in transcriptional repression, insulator function, and imprinting genetic information. Among the multitude of normal CTCF functions, its involvement in partitioning the genome into active or inactive domains by means of a chromatin insulator function has received particular attention. As an insulator CTCF helps to define the boundaries between the neighbouring genes. Strikingly, all vertebrate chromatin insulators identified so far interact with CTCF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTCF
• Entrez:
  • 10664(Human)
• Uniprot:
  • NP_006556.1(Human)