CXCL16 Antibody - BSA Free

Western Blot: CXCL16 Antibody [NBP2-16042] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: CXCL16 Antibody [NBP2-16042] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: CXCL16 Antibody [NBP2-16042] - Mouse thymus gland. CXCL16 stained by CXCL16 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: CXCL16 Antibody [NBP2-16042] - Gastric cancer. CXCL16 antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

CXCL16 Antibody [NBP2-16042] - Mouse spleen. CXCL16 stained by CXCL16 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CXCL16 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human CXCL16. The exact sequence is proprietary.
• Localization: Cell membrane; Single-pass type I membrane protein; Secreted; Cell membrane; Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CXCL16
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen Assay dependent
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for CXCL16 Antibody - BSA Free

• chemokine (C-X-C motif) ligand 16
• CXC chemokine ligand 16
• CXCL16
• CXCLG16
• Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein
• SCYB16
• Small-inducible cytokine B16
• SRPSOXC-X-C motif chemokine 16
• SR-PSOXTransmembrane chemokine CXCL16

Background

Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CXCL16
• Uniprot:
  • Q9H2A7(Human)