Cyclin D1 Antibody - BSA Free

Western Blot: Cyclin D1 Antibody [NBP2-16054] - Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody [NBP2-16054] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cyclin D1 protein stained by Cyclin D1 antibody [N1C3] diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Scale bar = 10 um.

Immunoprecipitation: Cyclin D1 Antibody [NBP2-16054] - Immunoprecipitation of Cyclin D1 protein from MCF-7 whole cell extracts using 5 ug of Cyclin D1 antibody [N1C3]. Western blot analysis was performed using Cyclin D1 antibody [N1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

ELISA: Cyclin D1 Antibody [NBP2-16054] - Detection of recombinant full-length Cyclin D1 protein using as capture antibody at concentration of 5 ug/mL and as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.

Western Blot: Cyclin D1 Antibody [NBP2-16054] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (NBP2-16054) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-16054] - Cyclin D1 antibody detects Cyclin D1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded cat liver.Cyclin D1 stained by Cyclin D1 antibody (NBP2-16054) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Cyclin D1 Antibody [NBP2-16054] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] (NBP2-16054) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Cyclin D1 Antibody [NBP2-16054] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody (NBP2-16054) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: Cyclin D1 Antibody [NBP2-16054] - Wild-type (WT) and Cyclin D1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D1 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Rt, Ca, Fe, Mu, Bv, Ca, RM
• Applications: WB, ELISA, ICC/IF, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cyclin D1 Antibody - BSA Free Summary

• Immunogen: Full length human Cyclin D1 Recombinant protein.
• Predicted Species: Mouse (93%), Rhesus Macaque (99%), Bovine (93%), Canine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCND1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:10000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockout Validated
  • Sandwich ELISA
  • Western Blot 1:500-1:3000
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (86%), Xenopus laevis (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Cyclin D1 Antibody - BSA Free

• B-cell lymphoma 1 protein
• BCL-1 oncogene
• Bcl-1
• BCL1D11S287E
• CCND1
• cyclin D1 (PRAD1: parathyroid adenomatosis 1)
• Cyclin D1
• Cyl-1
• G1/S-specific cyclin D1
• G1/S-specific cyclin-D1
• PRAD1 oncogene
• PRAD1
• PRAD1B-cell CLL/lymphoma 1
• U21B31

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cyclin D1 Antibody (NBP2-16054)(1)

Publication using NBP2-16054

• Lee JH, Ju-Hyun A, Youn HY Tumour necrosis factor stimulated gene 6 intrinsically regulates PD-L1 expressions in breast cancer cells, leading to modulation of tumour microenvironment Veterinary and comparative oncology 2023-02-20 [PMID: 36807440]

Bioinformatics

• Gene Symbol: CCND1
• Uniprot:
  • P24385()