CYP8B1 Antibody (RB17098)

Product Discontinued

CYP8B1 Antibody (RB17098) Summary

• Immunogen: This CYP8B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 471-501 amino acids from the C-terminal region of human CYP8B1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CYP8B1
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:50
  • Western Blot 1:1000
• Theoretical MW: 58.068 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for CYP8B1 Antibody (RB17098)

• CP8B
• CYP127 alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase
• CYPVIIIB1
• Cytochrome P450 8B1
• cytochrome P450, family 8, subfamily B, polypeptide 1
• cytochrome P450, subfamily VIIIB (sterol 12-alpha-hydroxylase), polypeptide 1,7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
• EC 1.14.13.95
• FLJ17826,7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase
• Sterol 12-alpha-hydroxylase

Background

CYP8B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP8B1
• Uniprot:
  • Q9UNU6(Human)