Cytokeratin 14 Antibody (LL001) [Unconjugated]

Cytokeratin 14 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (Catalog # MAB3164) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Cytokeratin 14 was detected in immersion fixed NHEK human normal epidermal keratinocytes using Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (Catalog # MAB3164) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). CKAP4/p63 was also detected using Sheep Anti-Human CKAP4/p63 Affinity-purified Polyclonal Antibody (Catalog # AF7355) and stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). Cells were counterstained with DAPI (blue). Specific staining of Cytokeratin 14 was localized to intermediate filaments. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Cytokeratin 14 was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (Catalog # MAB3164) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: LL001
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cytokeratin 14 Antibody (LL001) [Unconjugated] Summary

• Immunogen: Human Cytokeratin 14 synthetic peptide
  DGKVVSTHEQVLRTKN
• Specificity: Detects human Cytokeratin 14 in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT14
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Western Blot

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cytokeratin 14 Antibody (LL001) [Unconjugated]

• CK14
• CK-14
• Cytokeratin 14
• cytokeratin-14
• EBS3
• EBS4
• K14
• keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
• keratin 14
• keratin, type I cytoskeletal 14
• keratin-14
• KRT14
• NFJ

Background

Cytokeratin 14, a type I intermediate filament protein, is one of the two keratins which distinguish stratifying epithelium from simple epithelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 14 Antibody (MAB3164)(3)

Publications using MAB3164

• G. Kenneth Gray, Carman Man-Chung Li, Jennifer M. Rosenbluth, Laura M. Selfors, Nomeda Girnius, Jia-Ren Lin et al. A human breast atlas integrating single-cell proteomics and transcriptomics Developmental Cell 6/1/2022 [PMID: 35617956]
• Meri Vattulainen, Tanja Ilmarinen, Taina Viheriälä, Vilma Jokinen, Heli Skottman Corneal epithelial differentiation of human pluripotent stem cells generates ABCB5+ and ∆Np63 alpha + cells with limbal cell characteristics and high wound healing capacity Stem Cell Research & Therapy 12/20/2021 [PMID: 34930437]
• Sun Z, Shushanov S, LeRoith D, Wood TL Decreased IGF type 1 receptor signaling in mammary epithelium during pregnancy leads to reduced proliferation, alveolar differentiation, and expression of insulin receptor substrate (IRS)-1 and IRS-2. Endocrinology, 2011-05-31;152(8):3233-45. 2011-05-31 [PMID: 21628386] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: KRT14