DBPA Antibody (PCRP-YBX3-2D12)

Analysis of Protein Array containing more than 19,000 full-length human proteins using DBPA antibody (PCRP-YBX3-2D12)

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with DBPA antibody (PCRP-YBX3-2D12). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Flow cytometric analysis of PFA-fixed HeLa cells. DBPA antibody (PCRP-YBX3-2D12) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using DBPA antibody (PCRP-YBX3-2D12) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: PCRP-YBX3-2D12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DBPA Antibody (PCRP-YBX3-2D12) Summary

• Description: Store at 2 to 8C. Non-hazardous. No MSDS required.
• Immunogen: Recombinant full-length human DBPA protein
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: YBX3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Paraffin 1-2ug/ml
  • Immunoprecipitation 1-2ug/100-500ug protein
  • Western Blot 1-2ug/ml
• Application Notes: Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for DBPA Antibody (PCRP-YBX3-2D12)

• cold shock domain protein A
• Cold shock domain-containing protein A
• cold-shock domain protein A
• CSDA1
• dbpA
• DBPAcold-shock domain containing A1
• DNA-binding protein A
• Single-strand DNA-binding protein NF-GMB
• ZO-1-associated nucleic acid-binding protein
• ZONAB

Background

DBPA binds to the GM-CSF promoter. Seems to act as a repressor. Binds also to full length mRNA and to short RNA sequences containing the consensus site 5'-UCCAUCA-3'. May have a role in translation repression

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YBX3