Recombinant Human Dihydrofolate Reductase/DHFR His Protein

SDS-Page: Recombinant Human Dihydrofolate Reductase/DHFR Protein [NBP1-30207] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Product Discontinued

Recombinant Human Dihydrofolate Reductase/DHFR His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-187 of Human Dihydrofolate Reductase/DHFR
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND

• Details of Functionality: Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.
• Source: E. coli
• Protein/Peptide Type: Biologically Active Protein
• Gene: DHFR
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Application Notes: Use in Functional Assay reported in scientific literature (PMID: 23707606)
• Theoretical MW: 23.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 2 mM DTT, 30% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Dihydrofolate Reductase/DHFR His Protein

• DHFR
• DHFRP1
• Dihydrofolate Reductase
• DYR
• EC 1.5.1.3

Background

DHFR, also known as Dihydrofolate reductase, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Recombinant Dihydrofolate reductase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Publications for Dihydrofolate Reductase/DHFR Biologically Active Protein (NBP1-30207)(1)

Publication using NBP1-30207

• Whitsett J, Rangel Filho A, Sethumadhavan S et al. Human endothelial dihydrofolate reductase low activity limits vascular tetrahydrobiopterin recycling. Free Radic Biol Med 2013-05-22 [PMID: 23707606] (Func)

Bioinformatics

• Gene Symbol: DHFR