Orthogonal Strategies: Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Staining in human kidney and skin tissues using anti-DDAH1 antibody. Corresponding ...read more
Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol.
Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Analysis in human cell line SH-SY5Y.
Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Staining of human kidney shows high expression.
Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Staining of human skin shows low expression as expected.
This antibody was developed against Recombinant Protein corresponding to amino acids: SKRTNQRGAEILADTFKDYAVSTVPVADGLHLKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYDKLTVPDDIAANCIYLNIPNKGHVLLHRTPEEYPESAKVYEKLKDHMLIPVSMSELEKVDGLLT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDAH1
Purity
Immunogen affinity purified
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IHC reported in scientific literature (PMID: 22440215). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Human reactivity reported in scientific literature (PMID: 22440215).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody - BSA Free
DDAH1
DDAH-1
DDAHdimethylargininase-1
DDAHI
Dimethylargininase-1
dimethylarginine dimethylaminohydrolase 1FLJ25539
EC 3.5.3.18
FLJ21264
N(G)
N(G)-dimethylarginine dimethylaminohydrolase 1
NG, NG-dimethylarginine dimethylaminohydrolase
Background
DDAH1 belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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