dsDNA Antibody (DSD/4054R)

Immunohistochemistry-Paraffin: dsDNA Antibody (DSD/4054R) [NBP3-07302] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with dsDNA Recombinant Rabbit Monoclonal Antibody (DSD/4054R).

Immunohistochemistry-Paraffin: dsDNA Antibody (DSD/4054R) [NBP3-07302] - Formalin-fixed, paraffin-embedded human tonsil stained with dsDNA Recombinant Rabbit Monoclonal Antibody (DSD/4054R).

Simple Western: dsDNA Antibody (DSD/4054R) [NBP3-07302] - Lane view of AAV samples probed with NBP3-07302 in Probe 1 of RePlex with chemiluminescence detection and the anti-VP1/2/3 antibody in Probe 2 of RePlex with NIR detection

Simple Western: dsDNA Antibody (DSD/4054R) [NBP3-07302] - Lane view of AAV9 samples probed with the anti-DNA antibody in Probe 1 of RePlex with chemiluminescence detection and the anti-VP1/2/3 antibody in Probe 2 of RePlex with NIR detection (left). Average peak areas of DNA detection were plotted from each serotype with error bars representing the standard deviations from the means (n=9) (right).

• Reactivity: Hu
• Applications: Simple Western, Flow, ICC/IF, IHC
• Clone: DSD/4054R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

dsDNA Antibody (DSD/4054R) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA % 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml.(NBP3-08490)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Nuclei of Burkitt's cells
• Localization: Nuclear
• Marker: Nuclear Marker
• Specificity: This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This monoclonal antibody recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This monoclonal antibody produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Simple Western
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for dsDNA Antibody (DSD/4054R)

• Double stranded DNA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for dsDNA Antibody (NBP3-07302)(3)

Publications using NBP3-07302

• Zhao D, Gao Y, Su Y et al. Oroxylin A regulates cGAS DNA hypermethylation induced by methionine metabolism to promote HSC senescence Pharmacological Research 2022-12-01 [PMID: 36464146] (ICC/IF)
• Zhao D, Gao Y, Su Y et al. Oroxylin A regulates cGAS DNA hypermethylation induced by methionine metabolism to promote HSC senescence Pharmacological Research 2022-12-01 [PMID: 36464146] (ICC/IF)
• Zhao D, Gao Y, Su Y et al. Oroxylin A regulates cGAS DNA hypermethylation induced by methionine metabolism to promote HSC senescence Pharmacological Research 2022 Dec 01 [PMID: 36464146] (ICC/IF)