DUOX1 Antibody - BSA Free

Western Blot: DUOX1 Antibody [NBP2-16232] - Boiled and unboiled A431 whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DUOX1 antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract).

Immunohistochemistry-Paraffin: DUOX1 Antibody [NBP2-16232] - Human gastric cancer, using DUOX1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: DUOX1 Antibody [NBP2-16232] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DUOX1 antibody [N1N3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Po, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DUOX1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human DUOX1. The exact sequence is proprietary.
• Localization: Apical cell membrane
• Predicted Species: Porcine (90%), Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DUOX1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 177 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Canine (86%), Rat (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for DUOX1 Antibody - BSA Free

• dual oxidase 1
• DUOX
• EC 1.11.1.-
• EC 1.6.3.1
• Large NOX 1
• LNOX1flavoprotein NADPH oxidase
• Long NOX 1
• MGC138840
• NADPH thyroid oxidase 1
• nicotinamide adenine dinucleotide phosphate oxidase
• NOXEF1
• THOX1MGC138841
• Thyroid oxidase 1

Background

The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX2. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for DUOX1 Antibody (NBP2-16232)(1)

Publication using NBP2-16232

• Poinsignon L, Chissey A, Ajjaji A et al. Placental cartography of NADPH oxidase (NOX) family proteins: Involvement in the pathophysiology of preeclampsia Archives of biochemistry and biophysics 2023-10-20 [PMID: 37866451] (Immunohistochemistry, Western Blot, Human)

Bioinformatics

• Gene Symbol: DUOX1
• Uniprot:
  • Q9NRD9(Human)