EBI2/GPR183 Antibody - BSA Free

Western Blot: EBI2/GPR183 Antibody [NBP2-27352] - Analysis of EBI2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide, and 3) rat brain lysate using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: EBI2/GPR183 Antibody [NBP2-27352] - Analysis of EBI2 in human stomach tumor tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.

• Reactivity: Hu, Pm, Pm
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

EBI2/GPR183 Antibody - BSA Free Summary

• Immunogen: A portion of amino acids 300-350 of human EBI2 was used as the immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPR183
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Western Blot 2-4ug/ml~

Reactivity Notes

Chimpanzee (100%), Mouse, Rat (93%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for EBI2/GPR183 Antibody - BSA Free

• EBI2
• EBI2Epstein-Barr virus induced gene 2 (lymphocyte-specific G protein-coupledreceptor)
• EBV-induced G-protein coupled receptor 2
• Epstein-Barr virus-induced G-protein coupled receptor 2
• G protein-coupled receptor 183
• GPR183
• G-protein coupled receptor 183

Background

G-protein coupled receptor 183 (EBI2) belongs to the G-protein coupled receptor 1 family of proteins. It is an orphan seven-transmembrane receptor originally identified as most up-regulated gene (>200-fold) in EBV-infected cells. It plays a central role during humoral immunity. It promotes activated B-cell localization in the outer follicle and interfollicular regions. Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses. It is expressed abundantly in lymphoid tissues such as spleen and lymph node, and in B- and T-lymphocytes. It is also reported to be highly expressed in lung, heart and gastrointestinal tract. EBI2 deficiency in mice led to a reduction in the early antibody response to a T-dependent antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPR183
• Uniprot:
  • P32249()