eIF2A [p Ser51] Antibody

Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2 alpha (Phospho-Ser51) antibody.

Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Paraffin-embedded human lung carcinoma tissue.

Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Western blot analysis of lysates from 1) KB, 2) MCF-7 cells, (Green) primary antibody was diluted at 1: 1000,

Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81896(left) or the same antibody preincubated with blocking peptide (right).

Immunocytochemistry/Immunofluorescence: eIF2A [p Ser51] Antibody [NB100-81896] - Staining of methanol-fixed HeLa cells using NB100-81896

• Reactivity: Hu, Mu, Rt, Po, Ca, Fi
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

eIF2A [p Ser51] Antibody Summary

• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human eIF2A around the phosphorylation site of serine 51 (E-L-SP-R-R).
• Modification: p Ser51
• Specificity: Detects endogenous levels of eIF2A only when phosphorylated at serine 51.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-200
  • Immunohistochemistry 1:100-1:300
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:2000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  eIF2A Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% glycerol, 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for eIF2A [p Ser51] Antibody

• EIF-2
• EIF-2A
• EIF2AEIF2
• EIF-2alpha
• eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• subunit 1 (alpha, 35kD )

Background

eIF2 alpha is a 36 kDa protein which is ubiquitously expressed in many cell types. The eIF2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. In mammalian cells, eIF2 alpha is phosphorylated at serine 51 (human EIF2 alpha, the equivalent residue in mouse is serine 52) by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF2 alpha blocks the GDP-GTP exchange activity of eIF2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF2 alpha is an important regulatory process in protein synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eIF2A Antibody (NB100-81896)(2)

Publications using NB100-81896

• Wang G, Xu J, Zhao J et al. Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice Nat Commun 2020-01-10 [PMID: 31924786] (IF/IHC, ICC/IF, Mouse)
• Liu YQ, Jia MQ, Xie ZH et al. Arrestins contribute to amyloid beta-induced cell death via modulation of autophagy and the alpha7nAch receptor in SH-SY5Y cells. Sci Rep. 2017-06-13 [PMID: 28611418] (WB, Human)

Bioinformatics

• Gene Symbol: EIF2A
• Entrez:
  • 83939(Human)
  • 229317(Mouse)
  • 502531(Rat)
• Uniprot:
  • P05198()