EIF3D Antibody

Western Blot: EIF3D Antibody [NB100-93299] - Detection of Human eIF3D/EIF3S7 on HeLa whole cell lysate. eIF3D/EIF3S7 was not efficiently immunoprecipitated by rabbit anti-eIF3D/EIF3S7 antibody NB100-93300.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

EIF3D Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 150 and 200 of human eukaryotic translation initiation factor 3, subunit D (eukaryotic translation initiation factor 3, subunit 7) using the numbering given in entry NP_003744.1
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF3D
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  EIF3D Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EIF3D Antibody

• eIF3d
• eIF3-p66
• EIF3S7eIF3 p66
• eIF-3-zeta
• eIF3-zeta
• Eukaryotic translation initiation factor 3 subunit 7
• eukaryotic translation initiation factor 3 subunit D
• eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa
• eukaryotic translation initiation factor 3, subunit D
• MGC126526
• MGC17258
• translation initiation factor eIF3 p66 subunit

Background

Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EIF3D Antibody (NB100-93299)(1)

Publication using NB100-93299

• Chazal PE, Daguenet E, Wendling C et al. EJC core component MLN51 interacts with eIF3 and activates translation Proc Natl Acad Sci U S A 2013-03-25 [PMID: 23530232] (WB, Human)

Bioinformatics

• Gene Symbol: EIF3D
• Entrez:
  • 8664(Human)
• Uniprot:
  • O15371(Human)