EIF3G Antibody

Western Blot: EIF3G Antibody [NB100-93298] - Detection of human eIF3G/EIF3S4 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-eIF3G/EIF3S4 antibody NB100-93298 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. eIF3G/EIF3S4 was also immunoprecipitated by two other rabbit anti-eIF3G/EIF3S4 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, Func, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

EIF3G Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human eukaryotic translation initiation factor 3, subunit G (eukaryotic translation initiation factor 3, subunit 4) using the numbering given in entry NP_003746.2 (G
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF3G
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay
  • Western Blot 1:2000-1:10000
• Application Notes: Use in PLA reported in scientific literature (PMID:34260917).
• Control:

  EIF3G Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25349259).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EIF3G Antibody

• eIF3 p42
• eIF3 p44
• eIF-3-delta
• eIF3-delta
• eIF3g
• EIF3-P42
• eIF3-p44
• EIF3S4eIF-3 RNA-binding subunit
• Eukaryotic translation initiation factor 3 RNA-binding subunit
• Eukaryotic translation initiation factor 3 subunit 4
• eukaryotic translation initiation factor 3 subunit G
• eukaryotic translation initiation factor 3 subunit p42
• eukaryotic translation initiation factor 3, subunit 4 (delta, 44kD)
• eukaryotic translation initiation factor 3, subunit 4 delta, 44kDa
• eukaryotic translation initiation factor 3, subunit G

Background

Eukaryotic initiation factor 3 subunit G (eIF3G) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5'-end of cellular mRNAs near the cap structure (m7GpppN). eIF3G has been shown to directly interact with AIF (apoptosis inducing factor), a protein that plays a role in caspase-independent apoptosis. AIF is able to inhibit protein synthesis via its interaction with eIF3g.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EIF3G Antibody (NB100-93298)(3)

Publications using NB100-93298

• Swarnkar S, Avchalumov Y, Espadas I Et Al. Molecular motor protein KIF5C mediates structural plasticity and long-term memory by constraining local translation Cell reports 2021-07-13 [PMID: 34260917] (PLA)
• Gao J, Schatton D, Martinelli P et al. CLUH regulates mitochondrial biogenesis by binding mRnAs of nuclear-encoded mitochondrial proteins. CLUH regulates mitochondrial biogenesis by binding mRnAs of nuclear-encoded mitochondrial proteins. 2014-10-27 [PMID: 25349259] (WB, Mouse, Human)
• Hutt DM, Roth DM, Vignaud H et al. The Histone Deacetylase Inhibitor, Vorinostat, Represses Hypoxia Inducible Factor 1 Alpha Expression through Translational Inhibition PLoS ONE 2014-08-28 [PMID: 25166596]

Bioinformatics

• Gene Symbol: EIF3G
• Entrez:
  • 8666(Human)
• Uniprot:
  • O75821(Human)