EIF4E3 Antibody

Product Discontinued

EIF4E3 Antibody Summary

• Immunogen: Synthetic peptides corresponding to unique epitope on eIF4E3. The peptide sequence was selected from amimo acids 1-70 C-terminal. These peptides were covalently modified post-synthetically to achieve desired antigenicity.
• Localization: The peptide sequence was selected from C-terminal, amimo acids 1-70
• Specificity: This antibody detects a single band of approximately 30kDa in PC-eIF4E3 samples.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF4E3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Western Blot 1:5000
• Application Notes: Thsi product is used for WB and ELISA applications, other applications have not been tested. The species cross reactivity for these antibodies have not been examined fully. The dilutions for this antibody is for reference only, investigators are expected to determine the optimal conditions for specific assay. WB: > 1:500; IMM & i.p pull-down assays: n.d; IHC n.d. ELISA <1:10,000. Application of this antibody in protocols not listed here does not necessarily

Reactivity Notes

This product is reactive in Drosophila.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.69 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EIF4E3 Antibody

• eIF4E type 3
• eIF-4E type 3
• eIF-4E3
• eIF4E-3
• eukaryotic translation initiation factor 4E family member 3
• eukaryotic translation initiation factor 4E member 3
• eukaryotic translation initiation factor 4E type 3
• MGC39820
• MGC86971

Background

The fly Drosophila melanogaster is one of the most intensively studied organisms in biology and serves as a model system for the investigation of many developmental and cellular processes such as, translation of mRNA which is common to higher eukaryotes, including humans. Translation is divided into three phases: initiation, elongation, and termination (1). Early in the initiation phase, mRNA is recruited to the ribosomes primarily by eukaryotic translation initiation factor 4E (eIF4E) which is a cap binding protein. eIF4E recognizes and binds to the 5',7-methylguanosine-containing mRNA cap early in initiation phase and this leads to recruitment of mRNA to the ribosomes and subsequent initiation of protein synthesis (2). To date, three different family members of eIF4E have been recognized in mammals, they are: eIF4E-1, eIF4E-2 and eIF4E-3. eIF4E-1 is found in all eukaryotes while eIF4E-2 and eIF4E-3 are found only in metazoans and chordates, respectively (3). The activity of eIF4E proteins is regulated by eIF4E-binding proteins which suppress the activity of eIF4E proteins (4). Once activated, eIF4E-3 has been shown to inhibit the activity of eIF4E-1 protein. It is also known to interact with initiation factor eIF4G (3). eIF4E proteins are implicated in controlling growth of cancer through the P13K/Akt/mTOR signaling pathway, via the phosphorylation of eIF4E-bindnig proteins (5). Once eIF4E-binding proteins are phosphorylated, they can no longer interact with eIF4E proteins and hence are not able to limit the expression of eIF4E proteins in cells. eIF4E proteins are not as abundant in mammalian cells as other initiating factors and any overexpression of these proteins has been shown to transform normal cells into to cancer cells (5). eIF4E-3 is expressed in heart, skeletal muscle, lungs and spleen. It is approximately a 30kDa protein (244 amino acids). The eIF4E3 antibodies were generated using peptide corresponding to N-terminal and C-terminal of Fruit Fly eIF4E3 protein. eIF4E3 antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF4E3
• Uniprot:
  • NP_648194(Drosophila)