Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free

Immunocytochemistry/Immunofluorescence: Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free [NBP1-05170] - Perlecan (green) (G-I) secretion follows to stabilize the laminin and collagen IV networks. Scalebar = 20 um in all confocal micrographs. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fcell.2019.00224/full), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Mouse Monoclonal Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free [NBP1-05170] - Human primary brain microvascular endothelial cells stained for PDGF-BB (NBP1-58279), red and HSPG (NBP1-05170), green and nuclear DAPI blue. Image from a verified customer review.

• Reactivity: Hu, Bv
• Applications: WB, ELISA, ICC/IF, IHC, IP, AP
• Clone: A76
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free Summary

• Immunogen: Lysed bovine corneal endothelial cells and extracellular matrix
• Specificity: Highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPG2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:8000
  • Immunoaffinity Purification
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Use in ICC/IF was reported in scientific literature (PMID: 31750298). IHC: NBP1-05170 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues. AP: NBP1-05170 is more sensitive than NBP2-23522 or NBP2-23523 in ELISA. (2) IP: NBP1-05170 can be used in immunoprecipitation.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) - BSA Free

• basement membrane-specific heparan sulfate proteoglycan core protein
• endorepellin (domain V region)
• heparan sulfate proteoglycan 2
• Heparan Sulfate Proteoglycan
• HSPG
• perlecan proteoglycan
• perlecan
• PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia)
• PRCAN
• SJA
• SJS
• SJS1

Background

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodeling (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (NBP1-05170)(3)

Publications using NBP1-05170

• Stankiewicz LN, Salim K, Flaschner EA, Wang YX et Al. Sex-biased human thymic architecture guides T cell development through spatially defined niches Dev Cell 2024-10-09 [PMID: 39383865]
• Pain S, Dos Santos M, Gaydon A, Boher A. Restoration of Both Epithelial and Endothelial Perlecan/Dystroglycan Expressions by Polygonum bistorta Induces Skin Rejuvenation. International Federation of Science of Cosmetic Chemists. 2014-03-01 (IHC-P, Human)
• Wu D, Witt RL, Harrington DA, Farach-Carson MC Dynamic Assembly of Human Salivary Stem/Progenitor Microstructures Requires Coordinated alpha1 beta1 Integrin-Mediated Motility Front Cell Dev Biol 2019-10-16 [PMID: 31750298] (ICC/IF, Human)

Review for Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (NBP1-05170) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: KARTHIKEYAN THIRUGNANAM ICC Human 01/23/2024

Summary

• Application: Immunocytochemistry
• Sample Tested: Brain microvascular endothelial cells
• Species: Human
• Lot: 07071T

Bioinformatics

• Gene Symbol: HSPG2