ErbB2/Her2 Antibody

Western Blot: ErbB2/Her2 Antibody [NB100-1710] - Detection of Human ErbB2 on HeLa whole cell lysate using NB100-1710. ErbB2 was also immunoprecipitated using rabbit anti-ErbB2 antibodies NB100-1711 and NB100-1712.

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NB100-1710] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ErbB2 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Ca
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ErbB2/Her2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 975 and 1025 of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 using the numbering given in entry NP_004439.2 (GeneID 2064).
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ERBB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Control:

  ErbB2/Her2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ErbB2/Her2 Antibody

• CD340 antigen
• CD340
• c-erb B2/neu protein
• EC 2.7.10
• EGFR2
• ErbB2
• HER2
• HER-2
• HER2EC 2.7.10.1
• herstatin
• Metastatic lymph node gene 19 protein
• MLN 19
• MLN19
• Neu Oncogene
• NEUHER-2/neu
• neuroblastoma/glioblastoma derived oncogene homolog
• NGL
• NGLTKR1
• p185erbB2
• Proto-oncogene c-ErbB-2
• Proto-oncogene Neu
• receptor tyrosine-protein kinase erbB-2
• TKR1
• Tyrosine kinase-type cell surface receptor HER2
• v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
• v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Background

ErbB2 is a member of the receptor tyrosine kinase family of proteins. The four members of this family are ErbB1 (Epidermal growth factor receptor; EGFR/HER1), ErbB2 (HER2/Neu), ErbB3 (HER3), and ErbB4 (HER4). The ErbB receptor members are single-pass transmembrane proteins with an extracellular ligand-binding domain and a cytoplasmic tyrosine-kinase domain. The epidermal growth factor (EGF) and its related proteins serve as the ligands for the ErbB receptors. Upon binding, the receptors heterodimerize, autophosphorylate and make contact with adapter molecules to transmit cellular signals involved in cell proliferation, survival, and differentiation. The ErbB2 receptor is the only member that does not interact with a ligand, but instead functions as a heterodimeric partner to coordinate signals from other ligand bound ErbB receptors. Amplification and/or overexpression of ErbB2 have been reported in many cancers. Alternate names for ErbB2 include receptor tyrosine-protein kinase erbB-2. p185erbB2, C-erbB-2, Neu proto-oncogene, tyrosine kinase-type cell surface receptor HER2, TKR1, c-erb B2, MLN 19, ERBB2, HER2, NEU, and NGL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ErbB2/Her2 Antibody (NB100-1710)(1)

Publication using NB100-1710

• Rayner K, Chen YX, Hibbert B, White D, Miller H, Postel EH, O'Brien ER. NM23-H2, an estrogen receptor beta-associated protein, shows diminished expression with progression of atherosclerosis. Am J Physiol Regul Integr Comp Physiol;292(2):R743-50. 2007-02-01 [PMID: 17272673]

FAQs for ErbB2/Her2 Antibody (NB100-1710). (Showing 1 - 1 of 1 FAQ).

1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
   • A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

Bioinformatics

• Gene Symbol: ERBB2
• Entrez:
  • 2064(Human)
• Uniprot:
  • NP_004439.2(Human)